Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,555,388 A→C 14.6% intergenic (+26/+48) sbcB → / ← PP_1366 exodeoxyribonuclease I/transcriptional regulator MvaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,555,3880AC14.6% 94.9 / 5.1 39intergenic (+26/+48)sbcB/PP_1366exodeoxyribonuclease I/transcriptional regulator MvaT
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base C (2/4);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTC  >  NC_002947/1555249‑1555528
                                                                                                                                           |                                                                                                                                            
ttCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGc                                                                                                                                     >  3:175736/1‑149 (MQ=255)
  cGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGc                                                                                                                                     <  5:140700/147‑1 (MQ=255)
      aacgcgcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttttt                                                                                                                               <  4:19980/146‑1 (MQ=255)
      aaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttttt                                                                                                                               <  1:205328/149‑1 (MQ=255)
       aGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGTTTttg                                                                                                                              <  2:184170/149‑1 (MQ=255)
       aGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTttg                                                                                                                              <  7:86969/149‑1 (MQ=255)
       aGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTttg                                                                                                                              <  3:112487/149‑1 (MQ=255)
                                                                      aaCTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAgg                                                                                                             >  8:192755/1‑103 (MQ=255)
                                                                      aaCTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAgg                                                                                                             <  7:192755/103‑1 (MQ=255)
                                                                           cATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTcg                                                          >  8:261541/1‑149 (MQ=255)
                                                                               cTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTcgccgc                                                      <  6:243540/149‑1 (MQ=255)
                                                                                tCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCgccgcc                                                     >  7:176464/1‑149 (MQ=255)
                                                                                          cAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTc                                                                                         <  3:236765/103‑1 (MQ=255)
                                                                                          cAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTc                                                                                         >  4:236765/1‑103 (MQ=255)
                                                                                           aGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTCTCAACCACGTAGCAGCCCCAAGTGGCtt                                          >  5:178310/1‑149 (MQ=255)
                                                                                              aaCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCg                                                                                                    <  3:233320/88‑1 (MQ=255)
                                                                                              aaCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCg                                                                                                    >  4:233320/1‑88 (MQ=255)
                                                                                               aCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCa                                      <  4:175736/149‑1 (MQ=255)
                                                                                                   tATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCActct                                  >  8:12354/1‑149 (MQ=255)
                                                                                                     ttCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTAGCCGCCCCAAGTGGCTTTCCACTCtt                                 >  5:83647/1‑148 (MQ=255)
                                                                                                        aaTCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCa                              <  8:176464/148‑1 (MQ=255)
                                                                                                              cTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCa                                                                                            <  3:148519/80‑1 (MQ=255)
                                                                                                              cTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCa                                                                                            >  4:148519/1‑80 (MQ=255)
                                                                                                              cTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGACCAGCTTTAAACCACGTCGCAGCCCAAAGTGGCTTTCCACTCTTTCAGGGTCtt                       >  6:264222/1‑149 (MQ=255)
                                                                                                               tGATGGCATATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGAGTATGCGGCTAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtg                      >  7:206188/1‑149 (MQ=255)
                                                                                                                  tGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTgg                   <  7:261541/149‑1 (MQ=255)
                                                                                                                      aGATAAACAAAAAGGGCGGCTATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTTTGCGGATAATGAGTCCAGCAGGGTTGCCCAGCTTTGAAGCACGTCGCCGGCCCATGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgc               <  6:83647/149‑1 (MQ=255)
                                                                                                                      aGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgc               >  6:9402/1‑149 (MQ=255)
                                                                                                                          aaaCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTGTCCACTCTTTCAGGGTCTAGTGGTTgccgcc           >  3:37619/1‑149 (MQ=255)
                                                                                                                            aCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                            >  1:205870/1‑130 (MQ=255)
                                                                                                                            aCAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                            <  2:205870/130‑1 (MQ=255)
                                                                                                                             cAAAAACGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCttt        >  4:185851/1‑149 (MQ=255)
                                                                                                                               aaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTgg      <  5:9402/149‑1 (MQ=255)
                                                                                                                               aaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTgg      <  3:123003/149‑1 (MQ=255)
                                                                                                                                aaaCGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACg                                                             <  2:149596/93‑1 (MQ=255)
                                                                                                                                aaaCGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACg                                                             >  1:149596/1‑93 (MQ=255)
                                                                                                                                  agggcGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCTGCTTTCTACCACGTCGCCGCCGCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGttt   <  5:264222/145‑1 (MQ=255)
                                                                                                                                  aCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGtt                 <  7:149694/135‑1 (MQ=255)
                                                                                                                                  aCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGtt                 >  8:149694/1‑135 (MQ=255)
                                                                                                                                          aaTGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc  <  3:179101/142‑1 (MQ=255)
                                                                                                                                          aaTGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc  >  4:179101/1‑142 (MQ=255)
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TTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTC  >  NC_002947/1555249‑1555528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑