Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,756 G→C 9.3% intergenic (‑276/+37) galP‑II ← / ← ttgC porin‑like protein/efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,7560GC 9.3% 127.3 / 4.2 43intergenic (‑276/+37)galP‑II/ttgCporin‑like protein/efflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base C (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGACAGG  >  NC_002947/1576617‑1576904
                                                                                                                                              |                                                                                                                                                    
ccgcAAAACGCACCGGGCGATAATACAAACGAATTTTCCGGTTGGTTTAAAATTACTCTTCTGGCGCGGCCACTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAgg                                                                                                                                                <  1:101562/149‑1 (MQ=25)
                gCGATAATCGAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGc                                                                                                                             <  2:182277/149‑1 (MQ=255)
                  gATAATCGAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttgtt                                                                                                                                    >  1:257405/1‑137 (MQ=38)
                  gATAATCGAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttgtt                                                                                                                                    <  2:257405/140‑4 (MQ=38)
                  gATAATCGAACGGAATGTTCCGGTTGCTT‑‑‑AATAACTCTTCTGCGGCGGCCTCTTCGCCGCAAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCATTCTGCTTTTCca                                                                                                                            >  4:94932/1‑148 (MQ=255)
                         gAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAACCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGcc                                                                                                                    >  4:6761/1‑149 (MQ=255)
                              gAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGAt                                                                                                                 >  3:82305/1‑147 (MQ=255)
                               aaTGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAg                                                                                                              >  6:264972/1‑149 (MQ=255)
                                aTGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGc                                                                                                             <  5:264972/149‑1 (MQ=255)
                                           ggtt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCTCCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGgtgt                                                                                                   <  3:94932/148‑1 (MQ=255)
                                            gtt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGAt                                                                                                                 >  4:80732/1‑133 (MQ=255)
                                            gtt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGAt                                                                                                                 <  3:80732/133‑1 (MQ=255)
                                            gtt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGttt                                                                                                 >  5:70331/1‑149 (MQ=255)
                                             tt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGgtgagat                                                                                                >  5:135155/1‑145 (MQ=255)
                                             tt‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGgt                                                                                                     <  6:135155/144‑1 (MQ=255)
                                                  aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCa                                                                                              >  1:310444/1‑149 (MQ=255)
                                                  aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCa                                                                                              >  1:198757/1‑149 (MQ=255)
                                                          ttctGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGcc                                                                                      >  6:321291/1‑149 (MQ=255)
                                                            ctGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGcc                                                                                      <  4:160055/147‑1 (MQ=255)
                                                               gggCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCctgctg                                                                                 >  4:250599/1‑149 (MQ=255)
                                                                       tctTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTgg                                                                                                                                            <  1:36594/82‑1 (MQ=255)
                                                                          gagcgGGTTTACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCtttt                                                                                                                               >  3:102736/3‑92 (MQ=255)
                                                                          gagcgGGTTTACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCtttt                                                                                                                               <  4:102736/90‑1 (MQ=255)
                                                                            gcgGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCg                                                                    <  2:310444/149‑1 (MQ=255)
                                                                               ggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTc                                                                 <  5:321291/149‑1 (MQ=255)
                                                                                            cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGc                                                                           <  4:218855/126‑1 (MQ=255)
                                                                                                gacgGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                                >  8:174206/1‑149 (MQ=255)
                                                                                                    gCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGcc                                             >  1:269823/1‑148 (MQ=255)
                                                                                                      ttGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAg                                          >  8:48851/1‑149 (MQ=255)
                                                                                                            ccAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTgg                                                                            <  4:68158/109‑1 (MQ=255)
                                                                                                            ccAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTgg                                                                            >  3:68158/1‑109 (MQ=255)
                                                                                                             cAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTg                                   >  7:38267/1‑149 (MQ=255)
                                                                                                                  ggCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAg                              <  6:70331/149‑1 (MQ=255)
                                                                                                                  ggCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAg                              <  8:38267/149‑1 (MQ=255)
                                                                                                                   gCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAgg                             <  2:20249/149‑1 (MQ=255)
                                                                                                                   gCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAgg                             >  1:197147/1‑149 (MQ=255)
                                                                                                                    cacaGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGt                            <  7:48851/149‑1 (MQ=255)
                                                                                                                      caGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGtt                           >  7:201451/1‑148 (MQ=255)
                                                                                                                               aaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCt                  <  2:197147/148‑1 (MQ=255)
                                                                                                                               aaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTg                 >  2:305367/1‑149 (MQ=255)
                                                                                                                                  agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCACCAAgc                                         <  5:21715/120‑1 (MQ=255)
                                                                                                                                  agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCACCAAgc                                         >  6:21715/3‑122 (MQ=255)
                                                                                                                                         ccACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTTCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGcc                                             <  2:202979/111‑1 (MQ=255)
                                                                                                                                              gATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGACAgg  <  3:250599/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTT‑‑‑AATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGACAGG  >  NC_002947/1576617‑1576904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑