Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,584,716 G→C 13.6% P100A (CCG→GCG)  PP_1388 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,584,7160GC13.6% 117.2 / 7.8 43P100A (CCG→GCG) PP_1388EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base C (3/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGA  >  NC_002947/1584575‑1584862
                                                                                                                                             |                                                                                                                                                  
ccGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTcc                                                                                                                                             <  2:200130/149‑1 (MQ=255)
  gcggcgTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                           <  5:192433/149‑1 (MQ=255)
  gaggcgTACATGCTGCTGATGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGTCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACAATCCCGCCTGCGCCGATACCCTGCAGTACACTGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                           <  7:166928/147‑1 (MQ=255)
            tgctgcTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                 >  7:132895/1‑149 (MQ=255)
             gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGgcg                                                                                                                                 >  2:178100/1‑148 (MQ=255)
             gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                 >  5:313011/1‑148 (MQ=255)
               tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                              >  5:73153/1‑149 (MQ=255)
               tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                              >  3:115880/1‑149 (MQ=255)
                gctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAgg                                                                                                                             >  7:76596/1‑149 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTTCCTTGATCAGCGAGACGATACCGGCTGCGGCGATACCCTGCAGTACACGGGCCATCAGCAATTGCGCCATGGCCCAGGACATGCCGCaat                                                                                                                             <  6:256359/148‑3 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                              <  7:91823/147‑1 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                              <  6:313011/147‑2 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                              <  8:132895/147‑2 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                               >  6:302754/1‑143 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                               <  5:302754/143‑1 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAAt                                                                                                                         <  1:178100/149‑1 (MQ=255)
                         gCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAg                                                                                                                     <  4:229847/148‑1 (MQ=255)
                               gTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                              >  2:9994/1‑149 (MQ=255)
                                      ccGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAg                                                                                                       <  3:258844/149‑1 (MQ=255)
                                        gcgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGc                                                                                                     <  1:9994/149‑1 (MQ=255)
                                         cgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCt                                                                                                    <  5:140453/149‑1 (MQ=255)
                                             cgcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGAt                                                                                                 <  6:73153/148‑1 (MQ=255)
                                              gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                               >  5:277499/1‑133 (MQ=255)
                                              gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                               <  6:277499/133‑1 (MQ=255)
                                                              tCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCg                                                                               >  6:144590/1‑149 (MQ=255)
                                                                gCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGgc                                                                             <  5:144590/149‑1 (MQ=255)
                                                                     tGATTGCCTGGCTCTCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCTATTGCGCCAAGTCGTGGGCCATGGGGCAGGCAATGGACTCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCCgccacgg                                                                        >  6:259056/1‑144 (MQ=255)
                                                                             tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                         >  4:99473/1‑108 (MQ=255)
                                                                             tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                         <  3:99473/108‑1 (MQ=255)
                                                                              ggCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAgg                                                               >  3:16813/1‑149 (MQ=255)
                                                                                   aCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGcc                                                          <  8:76596/149‑1 (MQ=255)
                                                                                             tCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                >  8:205911/1‑149 (MQ=255)
                                                                                                cGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                >  1:234601/1‑146 (MQ=255)
                                                                                                   cTGCGCAGATAACCTGCAGGACACGGGACAGCACCAATTGCGGGATGTCGTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACAGGTTAGGATCATCCTGAGCCGGCCGTTAAGGTCGCCCAGTTTGCCGTAGATc                                          <  4:16813/149‑1 (MQ=255)
                                                                                                       gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                              >  7:12164/1‑77 (MQ=255)
                                                                                                       gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                              <  8:12164/77‑1 (MQ=255)
                                                                                                            tACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGcc                                 >  3:160490/1‑149 (MQ=255)
                                                                                                              cccTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATCGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCa                                <  4:160490/148‑1 (MQ=255)
                                                                                                                         acGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGcca                    >  5:43476/1‑149 (MQ=255)
                                                                                                                           gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                 >  7:258488/1‑118 (MQ=255)
                                                                                                                           gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                 <  8:258488/118‑1 (MQ=255)
                                                                                                                                 gCACCAATTGCGGCATGTCCTGGGACATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATaa            <  4:274138/149‑1 (MQ=255)
                                                                                                                                           cGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGa  >  1:59870/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CCGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGA  >  NC_002947/1584575‑1584862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑