Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,584,721 T→G 11.0% D98A (GAC→GCC)  PP_1388 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,584,7210TG11.0% 118.1 / 5.4 44D98A (GAC→GCC) PP_1388EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base G (2/3);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAGG  >  NC_002947/1584575‑1584868
                                                                                                                                                  |                                                                                                                                                   
ccGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTcc                                                                                                                                                   <  2:200130/149‑1 (MQ=255)
  gcggcgTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                                 <  5:192433/149‑1 (MQ=255)
  gaggcgTACATGCTGCTGATGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGTCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACAATCCCGCCTGCGCCGATACCCTGCAGTACACTGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                                 <  7:166928/147‑1 (MQ=255)
            tgctgcTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                       >  7:132895/1‑149 (MQ=255)
             gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGgcg                                                                                                                                       >  2:178100/1‑148 (MQ=255)
             gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                       >  5:313011/1‑148 (MQ=255)
               tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                    >  5:73153/1‑149 (MQ=255)
               tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                    >  3:115880/1‑149 (MQ=255)
                gctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAgg                                                                                                                                   >  7:76596/1‑149 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTTCCTTGATCAGCGAGACGATACCGGCTGCGGCGATACCCTGCAGTACACGGGCCATCAGCAATTGCGCCATGGCCCAGGACATGCCGCaat                                                                                                                                   <  6:256359/148‑3 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                    <  7:91823/147‑1 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                                    <  6:313011/147‑2 (MQ=255)
                 ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                                    <  8:132895/147‑2 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                                     >  6:302754/1‑143 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                                     <  5:302754/143‑1 (MQ=255)
                    aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAAt                                                                                                                               <  1:178100/149‑1 (MQ=255)
                         gCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAg                                                                                                                           <  4:229847/148‑1 (MQ=255)
                               gTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                    >  2:9994/1‑149 (MQ=255)
                                      ccGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAg                                                                                                             <  3:258844/149‑1 (MQ=255)
                                        gcgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGc                                                                                                           <  1:9994/149‑1 (MQ=255)
                                         cgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCt                                                                                                          <  5:140453/149‑1 (MQ=255)
                                             cgcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGAt                                                                                                       <  6:73153/148‑1 (MQ=255)
                                              gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                                     >  5:277499/1‑133 (MQ=255)
                                              gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                                     <  6:277499/133‑1 (MQ=255)
                                                              tCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCg                                                                                     >  6:144590/1‑149 (MQ=255)
                                                                gCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGgc                                                                                   <  5:144590/149‑1 (MQ=255)
                                                                     tGATTGCCTGGCTCTCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCTATTGCGCCAAGTCGTGGGCCATGGGGCAGGCAATGGACTCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCCgccacgg                                                                              >  6:259056/1‑144 (MQ=255)
                                                                             tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                               >  4:99473/1‑108 (MQ=255)
                                                                             tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                               <  3:99473/108‑1 (MQ=255)
                                                                              ggCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAgg                                                                     >  3:16813/1‑149 (MQ=255)
                                                                                   aCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGcc                                                                <  8:76596/149‑1 (MQ=255)
                                                                                             tCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                      >  8:205911/1‑149 (MQ=255)
                                                                                                cGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                      >  1:234601/1‑146 (MQ=255)
                                                                                                   cTGCGCAGATAACCTGCAGGACACGGGACAGCACCAATTGCGGGATGTCGTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACAGGTTAGGATCATCCTGAGCCGGCCGTTAAGGTCGCCCAGTTTGCCGTAGATc                                                <  4:16813/149‑1 (MQ=255)
                                                                                                       gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                    >  7:12164/1‑77 (MQ=255)
                                                                                                       gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                    <  8:12164/77‑1 (MQ=255)
                                                                                                            tACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGcc                                       >  3:160490/1‑149 (MQ=255)
                                                                                                              cccTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATCGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCa                                      <  4:160490/148‑1 (MQ=255)
                                                                                                                         acGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGcca                          >  5:43476/1‑149 (MQ=255)
                                                                                                                           gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                       >  7:258488/1‑118 (MQ=255)
                                                                                                                           gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                       <  8:258488/118‑1 (MQ=255)
                                                                                                                                 gCACCAATTGCGGCATGTCCTGGGACATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATaa                  <  4:274138/149‑1 (MQ=255)
                                                                                                                                           cGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGa        >  1:59870/1‑149 (MQ=255)
                                                                                                                                              cATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGATCAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGAccc     >  2:237827/1‑149 (MQ=255)
                                                                                                                                                  tCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAgg  <  2:234601/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAGG  >  NC_002947/1584575‑1584868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑