Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,584,724 T→C 11.1% Q97R (CAG→CGG) ‡ PP_1388 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,584,7240TC11.1% 116.3 / 5.5 45Q97R (CAG→CGG) ‡PP_1388EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base C (2/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAGGC  >  NC_002947/1584577‑1584869
                                                                                                                                                   |                                                                                                                                                 
gcggcgTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                                  <  5:192433/149‑1 (MQ=255)
gaggcgTACATGCTGCTGATGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGTCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACAATCCCGCCTGCGCCGATACCCTGCAGTACACTGGCCAGCACCAATTGCGGCATGTCCTg                                                                                                                                                  <  7:166928/147‑1 (MQ=255)
          tgctgcTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                        >  7:132895/1‑149 (MQ=255)
           gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGgcg                                                                                                                                        >  2:178100/1‑148 (MQ=255)
           gctgctGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGccg                                                                                                                                        >  5:313011/1‑148 (MQ=255)
             tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                     >  5:73153/1‑149 (MQ=255)
             tgctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                     >  3:115880/1‑149 (MQ=255)
              gctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAgg                                                                                                                                    >  7:76596/1‑149 (MQ=255)
               ctgAAGTAGCCCTGGTAGCGGCCTCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTTCCTTGATCAGCGAGACGATACCGGCTGCGGCGATACCCTGCAGTACACGGGCCATCAGCAATTGCGCCATGGCCCAGGACATGCCGCaat                                                                                                                                    <  6:256359/148‑3 (MQ=255)
               ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAg                                                                                                                                     <  7:91823/147‑1 (MQ=255)
               ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                                     <  8:132895/147‑2 (MQ=255)
               ctgAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGCCATGGCCCAGGACATGCCGCaa                                                                                                                                     <  6:313011/147‑2 (MQ=255)
                  aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                                      >  6:302754/1‑143 (MQ=255)
                  aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCa                                                                                                                                      <  5:302754/143‑1 (MQ=255)
                  aaGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAAt                                                                                                                                <  1:178100/149‑1 (MQ=255)
                       gCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAg                                                                                                                            <  4:229847/148‑1 (MQ=255)
                             gTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                     >  2:9994/1‑149 (MQ=255)
                                    ccGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAg                                                                                                              <  3:258844/149‑1 (MQ=255)
                                      gcgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGc                                                                                                            <  1:9994/149‑1 (MQ=255)
                                       cgTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCt                                                                                                           <  5:140453/149‑1 (MQ=255)
                                           cgcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGAt                                                                                                        <  6:73153/148‑1 (MQ=255)
                                            gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                                      >  5:277499/1‑133 (MQ=255)
                                            gcgcgGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAgg                                                                                                                      <  6:277499/133‑1 (MQ=255)
                                                            tCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCg                                                                                      >  6:144590/1‑149 (MQ=255)
                                                              gCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGgc                                                                                    <  5:144590/149‑1 (MQ=255)
                                                                   tGATTGCCTGGCTCTCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCTATTGCGCCAAGTCGTGGGCCATGGGGCAGGCAATGGACTCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCCgccacgg                                                                               >  6:259056/1‑144 (MQ=255)
                                                                           tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                                >  4:99473/1‑108 (MQ=255)
                                                                           tGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTTCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAAc                                                                                                                <  3:99473/108‑1 (MQ=255)
                                                                            ggCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAgg                                                                      >  3:16813/1‑149 (MQ=255)
                                                                                 aCCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGcc                                                                 <  8:76596/149‑1 (MQ=255)
                                                                                           tCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                       >  8:205911/1‑149 (MQ=255)
                                                                                              cGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCg                                                       >  1:234601/1‑146 (MQ=255)
                                                                                                 cTGCGCAGATAACCTGCAGGACACGGGACAGCACCAATTGCGGGATGTCGTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACAGGTTAGGATCATCCTGAGCCGGCCGTTAAGGTCGCCCAGTTTGCCGTAGATc                                                 <  4:16813/149‑1 (MQ=255)
                                                                                                     gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                     >  7:12164/1‑77 (MQ=255)
                                                                                                     gcCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAggg                                                                                                                     <  8:12164/77‑1 (MQ=255)
                                                                                                          tACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGcc                                        >  3:160490/1‑149 (MQ=255)
                                                                                                            cccTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATCGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCa                                       <  4:160490/148‑1 (MQ=255)
                                                                                                                       acGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGcca                           >  5:43476/1‑149 (MQ=255)
                                                                                                                         gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                        >  7:258488/1‑118 (MQ=255)
                                                                                                                         gggCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGcc                                                        <  8:258488/118‑1 (MQ=255)
                                                                                                                               gCACCAATTGCGGCATGTCCTGGGACATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATaa                   <  4:274138/149‑1 (MQ=255)
                                                                                                                                         cGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGa         >  1:59870/1‑149 (MQ=255)
                                                                                                                                            cATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGATCAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGAccc      >  2:237827/1‑149 (MQ=255)
                                                                                                                                                tCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAgg   <  2:234601/148‑1 (MQ=255)
                                                                                                                                                  cTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAGGc  >  7:209625/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGGCGTACATGCTGCTGAAGTAGCCCTGGTAGCGGCCGCGTTCGCGCGGGGGCACGAAGTCGCCAATGATTGCCTGGCTCACCGAGACCATCCCGCCTGCGCCGATACCCTGCAGTACACGGGCCAGCACCAATTGCGGCATGTCCTGGGCCATGGCGCAGGCAATGGAGGCCAGGGTGAACAGGCTGATACCGGTGAGGATCATCCGGCGCCGGCCGTAAAGGTCGCCCAGTTTGCCGTAGATCGGCACGGCCACGGTCATGGCCACCATATAACCGGAGATGACCCAGGC  >  NC_002947/1584577‑1584869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑