Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,585,171 G→A 7.3% intergenic (‑158/‑218) PP_1388 ← / → PP_1389 EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,585,1710GA 7.3% 157.6 / 2.5 55intergenic (‑158/‑218)PP_1388/PP_1389EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (28/23);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GTTTTGTCTATGGCGGCCCTTCGCGGGCAAGCCTGCTCTCACAGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGAC  >  NC_002947/1585025‑1585316
                                                                                                                                                  |                                                                                                                                                 
gTTTTGTCTATGGCGGCCCTTCGCGGGCAAGCCTGCTCTGACAGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCg                                                                                                                                                   <  5:272493/147‑1 (MQ=255)
   ttGTCTATGGCGGCCCTTCGCGGGCAAGCCTGCTCTCACAGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGTGTGat                                                                                                                                               <  8:52663/148‑1 (MQ=255)
            gcggcCCTTCGCGGGCAAGCCTGCTCTCACAGAAGCCCTCGGATTGTAACCTCAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTa                                                                                                                                     >  8:269352/1‑149 (MQ=255)
                            aaGCCTGCTCTCACTGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATCCCACAGAGGCTGaa                                                                                                                     <  7:269352/149‑1 (MQ=255)
                                      tcACAGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCt                                                                                                           >  6:126150/1‑149 (MQ=255)
                                        acaGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTg                                                                                                         >  1:279149/1‑149 (MQ=255)
                                            aaGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCAcc                                                                                                     <  5:126150/149‑1 (MQ=255)
                                            aaGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCAc                                                                                                      >  6:70726/1‑148 (MQ=255)
                                              gCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAg                                                                                                   >  1:233548/1‑149 (MQ=255)
                                                 cTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGtt                                                                                                >  6:220657/1‑149 (MQ=255)
                                                    ggATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTgg                                                                                              <  2:279149/148‑1 (MQ=255)
                                                    ggATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTg                                                                                               <  5:220657/147‑1 (MQ=255)
                                                          tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCtt                                                                                                          <  6:1370/130‑1 (MQ=255)
                                                          tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCtt                                                                                                          >  5:1370/1‑130 (MQ=255)
                                                           aaCCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgcagca                                                                                       >  2:206290/1‑148 (MQ=255)
                                                            aCCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCata                                                                                     >  2:289552/1‑149 (MQ=255)
                                                             ccTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCatat                                                                                    >  1:89336/1‑149 (MQ=255)
                                                                 aGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCATGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGcc                                                                                >  2:143969/1‑149 (MQ=255)
                                                                 aGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCATGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGcc                                                                                >  2:148710/1‑149 (MQ=255)
                                                                          gCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttt                                                                        <  5:70726/148‑1 (MQ=255)
                                                                           cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCata                                                                                     >  2:148955/1‑134 (MQ=255)
                                                                           cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCata                                                                                     <  1:148955/134‑1 (MQ=255)
                                                                           cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                      <  2:233548/149‑1 (MQ=255)
                                                                                tATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTc                                                                 >  4:143386/1‑149 (MQ=255)
                                                                                tATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTc                                                                 >  1:33421/1‑149 (MQ=255)
                                                                                  tGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTcc                                                                <  2:33421/148‑1 (MQ=255)
                                                                                            gCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCca                                                     >  5:206209/1‑149 (MQ=255)
                                                                                            gCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGAATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCAGCTGTCTCCca                                                     >  1:218168/1‑149 (MQ=255)
                                                                                             cATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCATGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTcc                                                       <  1:148710/146‑1 (MQ=255)
                                                                                             cATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCATGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTcc                                                       <  1:143969/146‑1 (MQ=255)
                                                                                                 cccTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCt                                                >  7:187224/1‑149 (MQ=255)
                                                                                                      tGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTa                                            <  1:206290/148‑1 (MQ=255)
                                                                                                          aGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCct                                       >  3:202858/1‑149 (MQ=255)
                                                                                                          aGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCct                                       <  1:60955/149‑1 (MQ=255)
                                                                                                           gATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCTCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTa                                            >  6:301773/1‑143 (MQ=255)
                                                                                                           gATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTa                                            <  5:301773/143‑1 (MQ=255)
                                                                                                            aTCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCctct                                     <  4:202858/149‑1 (MQ=255)
                                                                                                             tCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCctct                                     <  6:206209/148‑1 (MQ=255)
                                                                                                              cGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTctgc                                   >  8:152508/1‑149 (MQ=255)
                                                                                                               ggCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTctgcc                                  <  8:187224/149‑1 (MQ=255)
                                                                                                               ggCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTctgc                                   <  2:89336/148‑1 (MQ=255)
                                                                                                                 cATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTctgcctg                                <  7:152508/149‑1 (MQ=255)
                                                                                                                      gcgcATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCGTGTTTTTCAGTCCTATGTCTCCCACACCTTTTATTCCTCTGCCTGCATg                            >  4:197894/1‑148 (MQ=255)
                                                                                                                      gcgcATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                      >  6:216035/1‑106 (MQ=255)
                                                                                                                      gcgcATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                      <  5:216035/106‑1 (MQ=255)
                                                                                                                      gcgcATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATg                            >  2:313197/1‑148 (MQ=255)
                                                                                                                       cgcATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTc                          <  3:143386/149‑1 (MQ=255)
                                                                                                                           ttCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGc                      <  1:289552/149‑1 (MQ=255)
                                                                                                                               tCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGa                  >  8:220247/1‑149 (MQ=255)
                                                                                                                                ccAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACATTTTATTCCTCTGCCTGCATGTCGAGCATGAc                 >  7:109722/1‑149 (MQ=255)
                                                                                                                                   gTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGc              >  1:96095/1‑149 (MQ=255)
                                                                                                                                   gTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGc              >  2:298552/1‑149 (MQ=255)
                                                                                                                                           tGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACATTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATgg      <  8:109722/149‑1 (MQ=255)
                                                                                                                                               tGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCAt                             >  7:159470/1‑122 (MQ=255)
                                                                                                                                               tGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCAt                             <  8:159470/122‑1 (MQ=255)
                                                                                                                                               tGCGTGATATAGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGAACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTTCCTGCATGTCGAGCATGACCGCACAGATGGCGAc  >  3:177883/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTGTCTATGGCGGCCCTTCGCGGGCAAGCCTGCTCTCACAGAAGCCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGAC  >  NC_002947/1585025‑1585316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑