Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,589,873 G→A 8.0% R221H (CGC→CAC)  PP_1394 → acetolactate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,589,8730GA 8.0% 142.7 / 2.4 50R221H (CGC→CAC) PP_1394acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

CCTGCTGCCGGGCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCTGGTGGCCGAGGCCGACGTGGTGCTGGC  >  NC_002947/1589727‑1590017
                                                                                                                                                  |                                                                                                                                                
ccTGCTGCCGGGCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGcc                                                                                                                                                >  4:165544/1‑149 (MQ=255)
    ctgcCGGGCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCATGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGc                                                                                                                                            <  3:214152/149‑1 (MQ=255)
       ccGGGCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCg                                                                                                                                          >  7:39426/1‑148 (MQ=255)
         gggCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGaa                                                                                                                                        >  2:238219/1‑148 (MQ=255)
               cccTGTACGTGGCAGTCGGGCTGGGGCTGCACCTCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCCTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCtt                                                                                                                                  >  1:71471/1‑148 (MQ=255)
                ccTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCtt                                                                                                                                  >  1:254319/1‑147 (MQ=255)
                ccTGTACGGGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCa                                                                                                                                <  7:130974/149‑1 (MQ=255)
                       gTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCg                                                                                                                         >  1:26687/1‑149 (MQ=255)
                         ggCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGt                                                                                                                       >  6:65394/1‑149 (MQ=255)
                         ggCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGt                                                                                                                       >  8:135752/1‑149 (MQ=255)
                                 ggctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGa                                                                                                               <  3:165544/149‑1 (MQ=255)
                                 ggctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGa                                                                                                               >  8:229817/1‑149 (MQ=255)
                                 ggctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGa                                                                                                               >  8:299560/1‑149 (MQ=255)
                                  gctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGa                                                                                                               <  1:133984/148‑1 (MQ=255)
                                  gctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGa                                                                                                               >  2:133984/1‑148 (MQ=255)
                                  gctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGAc                                                                                                              <  8:39426/149‑1 (MQ=255)
                                   ctggggctgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGAcc                                                                                                             <  1:174094/149‑1 (MQ=255)
                                         ctgCACCGCAGGCCGTGGCGCAGATGGCTTAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCATGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAAt                                                                                                       <  1:16343/149‑1 (MQ=255)
                                          tgCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATg                                                                                                      >  6:71101/1‑149 (MQ=255)
                                            cACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGc                                                                                                     >  2:106406/1‑148 (MQ=255)
                                                    gCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCtt                                                                                            >  4:247141/1‑149 (MQ=255)
                                                     ccGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTtg                                                                                           >  4:91706/1‑149 (MQ=255)
                                                       gTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTtgct                                                                                         <  2:26687/149‑1 (MQ=255)
                                                       gTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTtgct                                                                                         >  3:178489/1‑149 (MQ=255)
                                                             cAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTgccagc                                                                                   >  5:36571/1‑149 (MQ=255)
                                                               gATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTgccagcca                                                                                 <  5:65394/149‑1 (MQ=255)
                                                                 tGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTgccagccagc                                                                               <  6:36571/149‑1 (MQ=255)
                                                                         cGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCAtt                                                                       <  5:71101/149‑1 (MQ=255)
                                                                          gACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTg                                                                      <  1:238219/149‑1 (MQ=255)
                                                                                 cAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCg                                                               <  3:247141/149‑1 (MQ=255)
                                                                                 cAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCg                                                               <  7:299560/149‑1 (MQ=255)
                                                                                  aGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAATGCTTGCTGCCAGCCAGCCACCCATTGCAGATCgg                                                              <  5:187751/149‑1 (MQ=255)
                                                                                       aCGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGa                                                         <  1:106406/149‑1 (MQ=255)
                                                                                         gTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGGCTGCCACCCATTGCAGATCGGCTCGAcc                                                       <  2:71471/149‑1 (MQ=255)
                                                                                         gTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACACCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTc                                                           >  7:8939/1‑145 (MQ=255)
                                                                                         gTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACACCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTc                                                           <  8:13467/145‑1 (MQ=255)
                                                                                         gTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACACCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTc                                                           <  8:8939/145‑1 (MQ=255)
                                                                                         gTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACACCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTc                                                           >  7:13467/1‑145 (MQ=255)
                                                                                             gCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGt                                                   >  3:184691/1‑149 (MQ=255)
                                                                                                gCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGt                                                <  2:254319/149‑1 (MQ=255)
                                                                                                     ttCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCTAGTCGTTAccc                                           <  7:135752/149‑1 (MQ=255)
                                                                                                          gCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCcac                                      <  4:178489/149‑1 (MQ=255)
                                                                                                          gCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCca                                       <  7:229817/148‑1 (MQ=255)
                                                                                                                    gggCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCtg                            >  4:113521/1‑149 (MQ=255)
                                                                                                                    gggCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCtg                            >  8:206209/1‑149 (MQ=255)
                                                                                                                           ggCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCTGGTggcc                      >  1:55194/1‑148 (MQ=255)
                                                                                                                                      ctgcggtgGCACGGCTGGCCGAACACCTTCAGGCCCCTGAGGCGCTGACCATCAATGCCAAAGGCTTGCGGCGAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCTCACGGGCGCTGGTGGCCGAGGCCGACgtg          <  4:49394/149‑1 (MQ=255)
                                                                                                                                      ctgcgctgGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACGCAGTGGTTACCCGCCACACGGGCGCTGGTGGCCGAGGCCGACgtg          <  1:278593/149‑1 (MQ=255)
                                                                                                                                              gCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCTGGTGGCCGAGGCCGACGTGGTGCTgg   <  2:94910/148‑1 (MQ=255)
                                                                                                                                              gCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCTGGTGGCCGAGGCCGACGTGGTGCTGGc  <  4:184691/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCTGCTGCCGGGCCGCCCTGTACGTGGCAGTCGGGCTGGGGCTGCACCGCAGGCCGTGGCGCAGATGGCTGAGCGACTGGCCAGTGCACGTCGGCCGCTGATTCTGGCCGGTGGCGGGGCGTTGGCTGCGGGCGCTGCGCTGGCACGCCTGGCCGAACACCTTCAGGCCCCGGTGGCGCTGACCATCAATGCCAAAGGCTTGCTGCCAGCCAGCCACCCATTGCAGATCGGCTCGACCCAGTCGTTACCCGCCACACGGGCGCTGGTGGCCGAGGCCGACGTGGTGCTGGC  >  NC_002947/1589727‑1590017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑