Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,596,373 A→T 6.1% L213Q (CTG→CAG)  dctD‑III ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,596,3730AT 6.1% 195.3 / 3.0 66L213Q (CTG→CAG) dctD‑IIIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (28/34);  new base T (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

GTATCACCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCA  >  NC_002947/1596230‑1596520
                                                                                                                                               |                                                                                                                                                   
gTATCACCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCg                                                                                                                                                >  1:272728/1‑149 (MQ=255)
     aCCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACTGTTCGGcca                                                                                                                                            >  8:245455/1‑147 (MQ=255)
     aCCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACTGTTCGGcca                                                                                                                                            <  7:245455/148‑2 (MQ=255)
     aCCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTc                                                                                                                                           >  6:61424/1‑149 (MQ=255)
         gCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGaa                                                                                                                                        >  2:175490/1‑148 (MQ=255)
          cAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGaa                                                                                                                                        >  8:250289/1‑147 (MQ=255)
          cAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGaa                                                                                                                                        <  7:250289/147‑1 (MQ=255)
          cAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACa                                                                                                                                      <  5:32722/149‑1 (MQ=255)
          cAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACa                                                                                                                                      <  5:37369/149‑1 (MQ=255)
            aCAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGAGTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGc                                                                                                                                    >  6:230225/1‑149 (MQ=255)
               gCTTGGCCTGTTGGGCCAGGGGCATACTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGAc                                                                                                                                 <  8:262/149‑1 (MQ=255)
                 ttGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACTGTTCGGccatgaga                                                                                                                                       >  8:86029/1‑135 (MQ=255)
                 ttGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACTGTTCGGccatgaga                                                                                                                                       <  7:86029/141‑7 (MQ=255)
                      cTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGa                                                                                                                          <  6:29922/149‑1 (MQ=255)
                        gTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTc                                                                                                                                                 >  6:316533/1‑124 (MQ=255)
                        gTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTc                                                                                                                                                 <  5:316533/124‑1 (MQ=255)
                         ttGGGCCAGGGGCATGCTCTCGATTTCGGCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCTGCCTCGAACAGCGACTCGGGGATg                                                                                                                        <  7:216360/148‑1 (MQ=255)
                           gggCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGc                                                                                                                      <  4:20472/148‑1 (MQ=255)
                              ccAGGGGCATGCTCTCGATATCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCcgcg                                                                                                                  >  1:189530/1‑149 (MQ=255)
                               cAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCcgcg                                                                                                                  >  6:31433/1‑148 (MQ=255)
                                 ggggCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAg                                                                                                               <  1:175490/149‑1 (MQ=255)
                                      aTGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAg                                                                                                          <  2:272728/149‑1 (MQ=255)
                                         ctctCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAggg                                                                                                        <  1:192150/148‑1 (MQ=255)
                                            tcGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTa                                                                                                     >  8:205931/1‑148 (MQ=255)
                                              gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGc                                                                                                                                    >  5:100334/1‑115 (MQ=255)
                                              gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGc                                                                                                                                    <  6:100334/115‑1 (MQ=255)
                                              gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACa                                                                                                   <  8:114594/148‑1 (MQ=255)
                                              gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACa                                                                                                   >  7:114594/1‑148 (MQ=255)
                                                tttCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCg                                                                                                                                                >  4:3432/1‑101 (MQ=255)
                                                tttCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCg                                                                                                                                                <  3:3432/101‑1 (MQ=255)
                                                         ggAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTggcg                                                                                       >  7:80648/1‑149 (MQ=255)
                                                          gAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGAGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTggcgg                                                                                      <  7:205931/149‑1 (MQ=255)
                                                            acacGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGtt                                                                                                             >  8:66396/1‑124 (MQ=255)
                                                            acacGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGtt                                                                                                             <  7:66396/124‑1 (MQ=255)
                                                                    ccACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGcc                                                                            <  5:64740/149‑1 (MQ=255)
                                                                      aCCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTc                                                                                                                                                 <  3:275492/78‑1 (MQ=255)
                                                                      aCCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTc                                                                                                                                                 >  4:275492/1‑78 (MQ=255)
                                                                          ttGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTc                                                                      <  6:108319/149‑1 (MQ=255)
                                                                                   tCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTgcagg                                                               >  3:126005/1‑147 (MQ=255)
                                                                                       gCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTGCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCgg                                                         <  7:59452/149‑1 (MQ=255)
                                                                                                   gTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTgcag                                                                <  8:42515/130‑1 (MQ=255)
                                                                                                   gTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTgcag                                                                >  7:42515/1‑130 (MQ=255)
                                                                                                   gTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTcc                                             >  5:211750/1‑149 (MQ=255)
                                                                                                       aCCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGaa                                                                         >  2:205105/1‑117 (MQ=255)
                                                                                                       aCCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGaa                                                                         <  1:205105/117‑1 (MQ=255)
                                                                                                        cccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGc                                         <  8:80648/148‑1 (MQ=255)
                                                                                                         ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTTGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGc                                         <  3:272707/147‑1 (MQ=255)
                                                                                                         ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTTGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGc                                         >  4:272707/1‑147 (MQ=255)
                                                                                                         ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCgg                                                         <  1:143952/131‑1 (MQ=255)
                                                                                                         ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCgg                                                         >  2:143952/1‑131 (MQ=255)
                                                                                                         ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGAGGGCCGCGCAGTTAAGGGCTACAAAGGGCTGGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCa                                       <  5:230225/149‑1 (MQ=255)
                                                                                                           tGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACgg                                                                                 <  6:251486/105‑1 (MQ=255)
                                                                                                           tGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACgg                                                                                 >  5:251486/1‑105 (MQ=255)
                                                                                                              gcgcCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGCCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCAACCGCTCCTTGCCACTGcc                                  >  7:8569/1‑149 (MQ=255)
                                                                                                                gcCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCgg                                <  7:231364/149‑1 (MQ=255)
                                                                                                                  ccGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCGGCGCAGTTAAGGGCTTCAAAGGGCTTCGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCGGCTCCTTGCCACTGCCGGtt                              >  5:202615/1‑149 (MQ=255)
                                                                                                                      gAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCa                                       <  3:193275/136‑1 (MQ=255)
                                                                                                                      gAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCa                                       >  4:193275/1‑136 (MQ=255)
                                                                                                                         ggCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGc                                   <  3:160681/137‑1 (MQ=255)
                                                                                                                         ggCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGc                                   >  4:160681/1‑137 (MQ=255)
                                                                                                                                  ctcATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAac               <  7:194708/148‑1 (MQ=255)
                                                                                                                                     aTGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCTTTTTTTTAAAGTTACGGTTTCAACGCTGATCAacac             <  4:126005/147‑1 (MQ=37)
                                                                                                                                           gAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCg     >  2:210357/1‑149 (MQ=255)
                                                                                                                                             aCAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCt                                            <  7:234893/108‑1 (MQ=255)
                                                                                                                                             aCAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCt                                            >  8:234893/1‑108 (MQ=255)
                                                                                                                                              cAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCa  <  5:61424/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTATCACCCGCAACAGCTTGGCCTGTTGGGCCAGGGGCATGCTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTTCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCA  >  NC_002947/1596230‑1596520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑