Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,622,915	A→G	6.9%	L148L (TTG→CTG) ‡	PP_1422 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,622,915	0	A	G	6.9%	211.5 / 4.7	72	L148L (TTG→CTG) ‡	PP_1422	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/35);  new base G (3/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

CAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGG  >  NC_002947/1622770‑1623060                                                                                                                                                   |                                                                                                                                                   cAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGt                                                                                                                                                 >  4:18685/1‑149 (MQ=255)  aGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTg                                                                                                                                                <  3:167744/149‑1 (MQ=255)    gCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAACGTGGc                                                                                                                                              <  7:286539/149‑1 (MQ=255)      cTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCt                                                                                                                                            <  8:299979/149‑1 (MQ=255)         gAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGt                                                                                                                                         >  2:33220/1‑149 (MQ=255)                 tGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGcc                                                                                                                                             >  5:69910/1‑137 (MQ=255)                 tGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGcc                                                                                                                                             <  6:69910/137‑1 (MQ=255)                  gTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTGGTcaacc                                                                                                                                    <  5:169254/145‑5 (MQ=255)                   tCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTga                                                                                                                                <  3:18685/148‑1 (MQ=255)                   tCCCCCAGCACCGGCGGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCTGCATAGATGAGTACCGGCA‑GCGCGCGAATCTGTTTGACCAGCCCACGCAAGGTGGCCTGGTCGGGTTTga                                                                                                                                <  8:229294/148‑1 (MQ=255)                     ccccAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATATATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGagag                                                                                                                             >  8:165073/1‑149 (MQ=255)                     ccccAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGagag                                                                                                                             <  1:311532/149‑1 (MQ=255)                              cGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGc                                                                                                                   >  6:293328/1‑149 (MQ=255)                               ggCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCACTCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGc                                                                                                                   <  1:253273/149‑1 (MQ=255)                                gCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GTAGCGa                                                                                                                 >  4:84708/1‑149 (MQ=255)                                          cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTg                                                                                                                                 >  2:297642/1‑124 (MQ=255)                                          cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTg                                                                                                                                 <  1:297642/124‑1 (MQ=255)                                          cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGAttt                                                                                                       <  5:301701/149‑1 (MQ=255)                                             gATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCg                                                                                                     >  6:291121/1‑148 (MQ=255)                                               tCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAg                                                                                                  <  3:210341/149‑1 (MQ=255)                                                cacaTAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGc                                                                                                 >  3:244302/1‑149 (MQ=255)                                                      aTGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGc                                                                                           >  5:121884/1‑149 (MQ=255)                                                          aGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGTCAGATTTCGTAGCTGATGCCCGc                                                                                       <  6:121884/149‑1 (MQ=255)                                                          aGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGc                                                                                       >  8:141675/1‑149 (MQ=255)                                                          aGGCAAACCGGGTCAGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGc                                                                                       <  7:63485/149‑1 (MQ=255)                                                            gCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCa                                                                                     >  3:83263/1‑149 (MQ=255)                                                            gCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCa                                                                                     >  6:20878/1‑149 (MQ=255)                                                                   gggTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAg                                                                              <  7:141675/149‑1 (MQ=255)                                                                   gggTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAg                                                                              <  5:20878/149‑1 (MQ=255)                                                                     gTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTCGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAgg                                                                             <  1:60596/149‑1 (MQ=255)                                                                          acaacaGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGATGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTg                                                                       <  4:244302/149‑1 (MQ=255)                                                                            aacaGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGc                                                                     >  5:123397/1‑149 (MQ=255)                                                                              caGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                    >  2:59307/1‑148 (MQ=255)                                                                               aGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCa                                                                  <  4:83263/149‑1 (MQ=255)                                                                                gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCTGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                    >  4:3505/1‑146 (MQ=255)                                                                                gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCTGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                    <  3:3505/146‑1 (MQ=255)                                                                                gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGAt                                                                                             >  1:118494/1‑121 (MQ=255)                                                                                gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGAt                                                                                             <  2:118494/121‑1 (MQ=255)                                                                                   cGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGata                                                              >  1:8547/1‑149 (MQ=255)                                                                                         gCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGc                                                                                       >  3:302477/1‑118 (MQ=255)                                                                                         gCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGc                                                                                       <  4:302477/118‑1 (MQ=255)                                                                                           tctTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAg                                                      >  5:28248/1‑149 (MQ=255)                                                                                             ttCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACtt                                                                                  >  5:171623/1‑119 (MQ=255)                                                                                             ttCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACtt                                                                                  <  6:171623/119‑1 (MQ=255)                                                                                                ggCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTc                                                  <  1:59307/148‑1 (MQ=255)                                                                                                ggCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTc                                                  <  5:293328/148‑1 (MQ=255)                                                                                                  cATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGc                                               <  2:8547/149‑1 (MQ=255)                                                                                                   aTAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAg                                                                                                  <  2:264856/97‑1 (MQ=255)                                                                                                   aTAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAg                                                                                                  >  1:264856/1‑97 (MQ=255)                                                                                                    tAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTc                                             <  6:123397/149‑1 (MQ=255)                                                                                                    tAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTc                                             >  6:268139/1‑149 (MQ=255)                                                                                                       aTGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATcgc                                                          <  8:153442/133‑1 (MQ=255)                                                                                                       aTGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATcgc                                                          >  7:153442/1‑133 (MQ=255)                                                                                                        tGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                    >  5:201037/1‑122 (MQ=255)                                                                                                        tGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                    <  6:201037/122‑1 (MQ=255)                                                                                                        ggAGGACCGGCA‑GCGCGCCACTCTGGGTGACCAGCCCACCCAAGGGGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATCTCGCGCAGTTTCTGCTCCACt                                         <  7:262845/148‑1 (MQ=255)                                                                                                         gAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTg                                        >  8:139300/1‑149 (MQ=255)                                                                                                              ccGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                   >  2:238266/1‑149 (MQ=255)                                                                                                               cGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                   >  2:268945/1‑148 (MQ=255)                                                                                                                         gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                         <  6:28248/149‑1 (MQ=255)                                                                                                                              tCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCa                                                                               <  3:198089/90‑1 (MQ=255)                                                                                                                              tCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCa                                                                               >  4:198089/1‑90 (MQ=255)                                                                                                                                aacccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAAt                  >  1:153450/6‑149 (MQ=255)                                                                                                                                 acccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                   >  5:235953/5‑131 (MQ=255)                                                                                                                                 acccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                   <  6:235953/127‑1 (MQ=255)                                                                                                                                     gACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCt             >  1:144135/1‑149 (MQ=255)                                                                                                                                      aCCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCTGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc            <  7:165073/149‑1 (MQ=255)                                                                                                                                          gCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATc        <  1:33220/149‑1 (MQ=255)                                                                                                                                          gCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACGTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGAt         >  2:239305/1‑148 (MQ=255)                                                                                                                                              acccaAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATc        >  3:275240/1‑145 (MQ=255)                                                                                                                                              acccaAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATc        <  4:275240/145‑1 (MQ=255)                                                                                                                                                ccaAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAgg  >  1:303403/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCA‑GCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGT‑GAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGG  >  NC_002947/1622770‑1623060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑