Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,632,097 A→G 7.3% E284G (GAG→GGG)  lepA → elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,632,0970AG 7.3% 158.0 / 2.8 55E284G (GAG→GGG) lepAelongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base G (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGA  >  NC_002947/1631952‑1632241
                                                                                                                                                 |                                                                                                                                                
ttGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGGAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGt                                                                                                                                               >  8:262953/1‑149 (MQ=255)
    tCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGa                                                                                                                                            <  1:166771/148‑1 (MQ=255)
    tCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAg                                                                                                                                           <  1:37187/149‑1 (MQ=255)
    tCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAg                                                                                                                                           >  1:209234/1‑149 (MQ=255)
                     ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAgg                                                                                                                                          <  1:87824/133‑1 (MQ=255)
                     ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAgg                                                                                                                                          >  2:87824/1‑133 (MQ=255)
                      tCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTc                                                                                                                            >  3:141108/1‑146 (MQ=255)
                                  acacCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaaga                                                                                                                        >  4:262711/1‑138 (MQ=255)
                                  acacCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaaga                                                                                                                        <  3:262711/138‑1 (MQ=255)
                                     cccAGACCGCTGATCTGAAAGGCGGCGAAGTAGTCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCGCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAg                                                                                                          <  2:4952/149‑1 (MQ=255)
                                      ccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAgg                                                                                                         >  7:232710/1‑149 (MQ=255)
                                       cAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGt                                                                                                        <  8:232710/149‑1 (MQ=255)
                                        aGACCGCTGATCTGAAAGCCGGCGCAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTc                                                                                                       <  7:262953/149‑1 (MQ=255)
                                            cGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACg                                                                                                   >  8:48305/1‑149 (MQ=255)
                                             gCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGc                                                                                                  <  4:286935/149‑1 (MQ=255)
                                                   cTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCt                                                                                            >  8:275488/1‑149 (MQ=255)
                                                      aaaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGc                                                                                              <  3:159457/144‑1 (MQ=255)
                                                      aaaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGc                                                                                              >  4:159457/1‑144 (MQ=255)
                                                       aaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCATGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGTCTTCAAGAAGATTAATCCGCAGGTCTACGCCGGCCTGtt                                                                                         <  7:199181/148‑1 (MQ=255)
                                                          ccGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                     <  3:40141/149‑1 (MQ=255)
                                                                     ggCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAg                                                                                                                >  8:39835/1‑111 (MQ=255)
                                                                     ggCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAg                                                                                                                <  7:39835/111‑1 (MQ=255)
                                                                      gCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCcgac                                                                         <  7:275488/149‑1 (MQ=255)
                                                                        ttCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCg                                                                                                >  4:119069/1‑124 (MQ=255)
                                                                        ttCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCg                                                                                                <  3:119069/124‑1 (MQ=255)
                                                                          catcatCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCATGTTCCCGGTCAGCTCcgacga                                                                       <  7:48305/147‑1 (MQ=255)
                                                                           atcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACtt                                                                    <  4:141108/149‑1 (MQ=255)
                                                                              atcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACGCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCATGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                 <  6:204560/149‑1 (MQ=255)
                                                                               tcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                >  1:9466/1‑149 (MQ=255)
                                                                                      cATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTcc                                                         <  2:209234/149‑1 (MQ=255)
                                                                                           aGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                <  4:286335/137‑1 (MQ=255)
                                                                                           aGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                >  3:286335/1‑137 (MQ=255)
                                                                                             gACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGc                                                  >  6:41134/1‑149 (MQ=255)
                                                                                                aTCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCGGACGACTTCGa                                                                 <  6:164150/131‑1 (MQ=255)
                                                                                                aTCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                 >  5:164150/1‑131 (MQ=255)
                                                                                                 tCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTg                                              >  8:179058/1‑149 (MQ=255)
                                                                                                   cACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTc                                                                   <  3:218586/126‑1 (MQ=255)
                                                                                                   cACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTc                                                                   >  4:218586/1‑126 (MQ=255)
                                                                                                   cACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGc                                             >  5:149641/1‑148 (MQ=255)
                                                                                                    aCGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTc                                                                             <  5:166256/115‑1 (MQ=255)
                                                                                                    aCGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTc                                                                             >  6:166256/1‑115 (MQ=255)
                                                                                                            ccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAc                                   >  3:170064/1‑149 (MQ=255)
                                                                                                             cGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAcc                                  <  7:179058/149‑1 (MQ=255)
                                                                                                                   gTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGaa                             <  1:125672/148‑1 (MQ=255)
                                                                                                                          cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcct                     >  4:212302/1‑149 (MQ=255)
                                                                                                                          cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcct                     >  3:110510/1‑149 (MQ=255)
                                                                                                                           ctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                    >  4:91947/1‑149 (MQ=255)
                                                                                                                                 ctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAAc                            >  7:212678/1‑135 (MQ=255)
                                                                                                                                 ctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAAc                            <  8:212678/135‑1 (MQ=255)
                                                                                                                                 ctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTg                >  1:74390/1‑147 (MQ=255)
                                                                                                                                  tgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaaga                                                                                                                        >  6:94434/1‑42 (MQ=255)
                                                                                                                                  tgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaaga                                                                                                                        <  5:94434/42‑1 (MQ=255)
                                                                                                                                    tCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAg             <  3:212302/147‑1 (MQ=255)
                                                                                                                                      cTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa         >  8:54345/1‑149 (MQ=255)
                                                                                                                                               tGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCCGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGa  >  1:92221/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGA  >  NC_002947/1631952‑1632241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑