| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,639,581 | T→C | 7.0% | intergenic (‑171/‑140) | czcR‑III ← / → PP_1439 | response regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,639,581 | 0 | T | C | 7.0% | 157.0 / 2.0 | 57 | intergenic (‑171/‑140) | czcR‑III/PP_1439 | response regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (26/27); new base C (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCT > NC_002947/1639434‑1639728 | ggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTa < 1:122458/149‑1 (MQ=255)ggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTa < 3:58454/149‑1 (MQ=255) gACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAg < 8:73583/149‑1 (MQ=255) cTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg < 5:318241/148‑1 (MQ=255) ccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc > 4:293516/1‑149 (MQ=255) cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTActc < 3:15294/149‑3 (MQ=37) cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAccc > 6:121930/1‑149 (MQ=255) aCATCAGGGTCAAGCGCCCCAATGTTACGGTCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAACCCCGTTCCTACAGGCGCGGGTTTACTCGCGaa > 1:189298/1‑147 (MQ=25) aCATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag > 2:4436/1‑149 (MQ=255) aTCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc > 4:215350/1‑149 (MQ=255) tCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc > 2:36766/1‑149 (MQ=255) cccAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCTGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGACCGAACAG‑TCcaca > 5:224142/1‑148 (MQ=255) aTGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAgg < 1:4436/149‑1 (MQ=255) ttACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCca < 1:36766/149‑1 (MQ=255) ttACGG‑CATGAAAAAAGGCCTGCGGTCCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGcc < 3:37696/148‑1 (MQ=255) cATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTg > 2:237105/1‑148 (MQ=255) gAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAagag > 7:1047/1‑116 (MQ=21) gAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAagag < 8:1047/116‑1 (MQ=21) aaaaaaGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGa < 8:7377/149‑1 (MQ=255) aaaaaGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGAc > 3:69294/1‑149 (MQ=255) aGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGc > 6:249010/1‑140 (MQ=255) aGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGc < 5:249010/140‑1 (MQ=255) ttGCGGTGAATATAACGTTTTTTTATTTCTGTTGTTATGTGGCTGTCTTTTCGGGCCTCTGCGCTATTAACCCCGCGCCTGTAGCAAGGGTTTAACCCGCTAATTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAAt < 4:13717/146‑1 (MQ=255) cTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAAt > 3:13717/1‑147 (MQ=255) gTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGcc > 7:248481/1‑149 (MQ=255) tGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGCTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTa < 1:237105/148‑1 (MQ=255) tgctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTc > 3:88068/1‑149 (MQ=255) ctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGAGACTAAAACCGCGCCTGTAGGAACGGGTTTACCCGCGAAGACGCCCGAAAAG‑TCCAAACAAGGCCACATTGCACGACAGAAATCCCCGCCTCACCATTCCGTTACCCAGCCCTCga > 2:111286/1‑148 (MQ=255) tgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGc > 6:237604/1‑148 (MQ=255) tgTTATCTGGCTGTATGGTCGGGCCTCTTCGCGTGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCt < 6:224142/149‑1 (MQ=255) tATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAg < 3:215350/148‑1 (MQ=255) cgatgtctgtctgtTCGGGCCTCTTCGCCATTAAACCCGCGCCTGTAGTAAGGGTTTTACCCGCGAAGTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGa < 7:254815/143‑1 (MQ=255) aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGcc < 5:323314/128‑1 (MQ=255) ggCTGTCTGTTCGGGCCTCTTAGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCCCATTGCAGGACTGTAATCCCCGCCTCCCCGTGCCGTTAGCCAGCCCTAGCTAGGAGGGc > 6:29091/1‑149 (MQ=255) gCTGTCTGTTGTGTCCTCTTCGGTAGTAAACCCGGGCCTGTAGCACCGGGTTTACCCGCGATGTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCATCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc < 4:69294/149‑1 (MQ=255) gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc < 5:237604/149‑1 (MQ=255) gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc > 8:280931/1‑149 (MQ=255) gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCACTAGGATGGcc < 3:293516/149‑1 (MQ=255) gtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGACCgtg > 3:268994/1‑149 (MQ=255) tctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt < 6:270084/149‑1 (MQ=255) gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAaga > 5:304041/1‑57 (MQ=255) gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAaga < 6:304041/57‑1 (MQ=255) gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTg > 8:83578/1‑149 (MQ=255) ttCGGGCCTCTTCGCGCGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCGACATTGCGGGACTGTAATCCCCGCCTCCCCTTGc > 5:5845/1‑114 (MQ=37) ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTc < 8:248481/146‑1 (MQ=255) ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCAGTGTCTGa > 8:206691/1‑149 (MQ=255) cGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGAt < 1:125803/148‑1 (MQ=255) gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGt > 5:34695/1‑149 (MQ=255) cgcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttatt < 5:121930/146‑1 (MQ=255) cgATTAAACCGGCGCCTTTAGTACCGGGTTTACCCGCGATTAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg < 1:111286/149‑1 (MQ=255) cgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg < 7:83578/149‑1 (MQ=255) gTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGc > 7:20166/1‑148 (MQ=255) aaaCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc < 4:129172/122‑1 (MQ=255) aaaCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGAAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc > 3:129172/1‑122 (MQ=255) ccTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCTCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGaa < 7:280931/149‑1 (MQ=255) tGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAcc > 2:117738/1‑149 (MQ=255) gTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGCGGCAAACACGATGAACCt > 6:195046/1‑149 (MQ=255) | GGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCT > NC_002947/1639434‑1639728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |