Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,586 A→G 7.3% intergenic (‑176/‑135) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5860AG 7.3% 152.6 / 2.7 56intergenic (‑176/‑135)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

CTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACC  >  NC_002947/1639442‑1639732
                                                                                                                                                 |                                                                                                                                                   
cTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                                                  <  5:318241/148‑1 (MQ=255)
      ccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                                           >  4:293516/1‑149 (MQ=255)
       cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTActc                                                                                                                                          <  3:15294/149‑3 (MQ=37)
       cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAccc                                                                                                                                          >  6:121930/1‑149 (MQ=255)
               aCATCAGGGTCAAGCGCCCCAATGTTACGGTCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAACCCCGTTCCTACAGGCGCGGGTTTACTCGCGaa                                                                                                                                     >  1:189298/1‑147 (MQ=25)
               aCATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                                                  >  2:4436/1‑149 (MQ=255)
                 aTCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                                >  4:215350/1‑149 (MQ=255)
                  tCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                                               >  2:36766/1‑149 (MQ=255)
                                cccAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCTGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGACCGAACAG‑TCcaca                                                                                                                 >  5:224142/1‑148 (MQ=255)
                                    aTGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAgg                                                                                                            <  1:4436/149‑1 (MQ=255)
                                       ttACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCca                                                                                                         <  1:36766/149‑1 (MQ=255)
                                       ttACGG‑CATGAAAAAAGGCCTGCGGTCCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGcc                                                                                                          <  3:37696/148‑1 (MQ=255)
                                              cATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTg                                                                                                    >  2:237105/1‑148 (MQ=255)
                                                 gAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAagag                                                                                                                                  <  8:1047/116‑1 (MQ=21)
                                                 gAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAagag                                                                                                                                  >  7:1047/1‑116 (MQ=21)
                                                  aaaaaaGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGa                                                                                               <  8:7377/149‑1 (MQ=255)
                                                   aaaaaGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGAc                                                                                              >  3:69294/1‑149 (MQ=255)
                                                       aGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGc                                                                                                   <  5:249010/140‑1 (MQ=255)
                                                       aGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGc                                                                                                   >  6:249010/1‑140 (MQ=255)
                                                           ttGCGGTGAATATAACGTTTTTTTATTTCTGTTGTTATGTGGCTGTCTTTTCGGGCCTCTGCGCTATTAACCCCGCGCCTGTAGCAAGGGTTTAACCCGCTAATTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAAt                                                                                        <  4:13717/146‑1 (MQ=255)
                                                           cTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAAt                                                                                        >  3:13717/1‑147 (MQ=255)
                                                                gTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGcc                                                                                 >  7:248481/1‑149 (MQ=255)
                                                                                tGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGCTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTa                                                                   <  1:237105/148‑1 (MQ=255)
                                                                                        tgctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTc                                                         >  3:88068/1‑149 (MQ=255)
                                                                                          ctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGAGACTAAAACCGCGCCTGTAGGAACGGGTTTACCCGCGAAGACGCCCGAAAAG‑TCCAAACAAGGCCACATTGCACGACAGAAATCCCCGCCTCACCATTCCGTTACCCAGCCCTCga                                                       >  2:111286/1‑148 (MQ=255)
                                                                                           tgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGc                                                       >  6:237604/1‑148 (MQ=255)
                                                                                           tgTTATCTGGCTGTATGGTCGGGCCTCTTCGCGTGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCt                                                      <  6:224142/149‑1 (MQ=255)
                                                                                              tATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAg                                                    <  3:215350/148‑1 (MQ=255)
                                                                                               cgatgtctgtctgtTCGGGCCTCTTCGCCATTAAACCCGCGCCTGTAGTAAGGGTTTTACCCGCGAAGTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGa                                                  <  7:254815/143‑1 (MQ=255)
                                                                                               aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGcc                                                                       <  5:323314/128‑1 (MQ=255)
                                                                                                   ggCTGTCTGTTCGGGCCTCTTAGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCCCATTGCAGGACTGTAATCCCCGCCTCCCCGTGCCGTTAGCCAGCCCTAGCTAGGAGGGc                                              >  6:29091/1‑149 (MQ=255)
                                                                                                    gCTGTCTGTTGTGTCCTCTTCGGTAGTAAACCCGGGCCTGTAGCACCGGGTTTACCCGCGATGTGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCATCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                             <  4:69294/149‑1 (MQ=255)
                                                                                                    gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                             >  8:280931/1‑149 (MQ=255)
                                                                                                    gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                             <  5:237604/149‑1 (MQ=255)
                                                                                                    gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCACTAGGATGGcc                                             <  3:293516/149‑1 (MQ=255)
                                                                                                       gtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGACCgtg                                          >  3:268994/1‑149 (MQ=255)
                                                                                                        tctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                                         <  6:270084/149‑1 (MQ=255)
                                                                                                           gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAaga                                                                                                                                   <  6:304041/57‑1 (MQ=255)
                                                                                                           gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAaga                                                                                                                                   >  5:304041/1‑57 (MQ=255)
                                                                                                           gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTg                                      >  8:83578/1‑149 (MQ=255)
                                                                                                            ttCGGGCCTCTTCGCGCGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCGACATTGCGGGACTGTAATCCCCGCCTCCCCTTGc                                                                        >  5:5845/1‑114 (MQ=37)
                                                                                                            ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTc                                        <  8:248481/146‑1 (MQ=255)
                                                                                                            ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCAGTGTCTGa                                     >  8:206691/1‑149 (MQ=255)
                                                                                                              cGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGAt                                    <  1:125803/148‑1 (MQ=255)
                                                                                                                 gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGt                                >  5:34695/1‑149 (MQ=255)
                                                                                                                        cgcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttatt                            <  5:121930/146‑1 (MQ=255)
                                                                                                                          cgATTAAACCGGCGCCTTTAGTACCGGGTTTACCCGCGATTAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg                       <  1:111286/149‑1 (MQ=255)
                                                                                                                          cgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg                       <  7:83578/149‑1 (MQ=255)
                                                                                                                             gTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGc                     >  7:20166/1‑148 (MQ=255)
                                                                                                                               aaaCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                             <  4:129172/122‑1 (MQ=255)
                                                                                                                               aaaCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGAAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                             >  3:129172/1‑122 (MQ=255)
                                                                                                                                        ccTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCTCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGaa         <  7:280931/149‑1 (MQ=255)
                                                                                                                                          tGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAcc       >  2:117738/1‑149 (MQ=255)
                                                                                                                                           gTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGCGGCAAACACGATGAACCt      >  6:195046/1‑149 (MQ=255)
                                                                                                                                              ggAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGa    <  6:34695/148‑1 (MQ=255)
                                                                                                                                               gAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGACCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGAcc  <  4:268994/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGG‑CATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAG‑TCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACC  >  NC_002947/1639442‑1639732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑