| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,642,271 | G→C | 13.0% | intergenic (‑99/+53) | PP_1442 ← / ← lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,642,271 | 0 | G | C | 13.0% | 158.1 / 13.4 | 54 | intergenic (‑99/+53) | PP_1442/lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Reads supporting (aligned to +/- strand): ref base G (23/24); new base C (3/4); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
GAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCG > NC_002947/1642126‑1642419 | gAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAgggg > 6:275862/1‑149 (MQ=255)gAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCAcggg > 1:287792/1‑149 (MQ=38) gCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGc < 5:275862/149‑1 (MQ=255) aGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCg > 7:227157/1‑148 (MQ=255) cGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTAccc < 8:177339/149‑1 (MQ=255) gCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGc < 2:287792/149‑1 (MQ=21) gAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCtt > 4:221572/1‑149 (MQ=255) aGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTg > 7:245490/1‑149 (MQ=255) gACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCcg > 7:134679/1‑149 (MQ=255) gaggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGa < 7:87792/149‑1 (MQ=255) aggagAGGTCATGGGGGCTCCGACGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGaa < 7:108130/149‑1 (MQ=255) gagaGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAggg < 6:133539/111‑1 (MQ=255) gagaGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAggg > 5:133539/1‑111 (MQ=255) gagGGCATGGTGGCGCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGGCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAgg < 1:193322/149‑1 (MQ=255) gTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCg > 4:70086/1‑149 (MQ=255) cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg > 8:51619/1‑126 (MQ=25) cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg < 7:51619/126‑1 (MQ=25) gtggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAg > 1:163047/1‑149 (MQ=255) cTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa < 2:163047/149‑1 (MQ=255) cGAAGGGTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTAGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAg < 1:47278/148‑1 (MQ=255) cGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa > 4:53548/1‑149 (MQ=255) gAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa > 5:41953/1‑148 (MQ=255) aaGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa < 8:227157/149‑1 (MQ=255) aGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAg < 8:245490/149‑1 (MQ=255) tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg > 8:138543/1‑130 (MQ=255) tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg < 7:138543/130‑1 (MQ=255) gCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACttt > 2:302450/1‑149 (MQ=255) gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc < 2:293441/139‑1 (MQ=255) gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc > 1:293441/1‑139 (MQ=255) tGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGcc < 1:302450/149‑1 (MQ=255) aGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCgg < 3:221572/149‑1 (MQ=255) aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg < 5:241526/100‑6 (MQ=17) aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg > 6:241526/1‑95 (MQ=17) ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGccc < 6:71383/97‑1 (MQ=255) ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc > 8:173535/1‑132 (MQ=255) ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc < 7:173535/132‑1 (MQ=255) cGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc > 4:192668/1‑122 (MQ=255) gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc > 2:30159/1‑146 (MQ=255) gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc < 1:30159/146‑1 (MQ=255) atCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc > 2:39046/1‑149 (MQ=255) aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGaaa > 5:111526/1‑149 (MQ=255) ctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAg < 3:70086/148‑1 (MQ=255) ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGcc < 6:111526/148‑1 (MQ=255) ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGc < 3:53548/147‑1 (MQ=255) ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCt > 3:259339/1‑149 (MQ=255) gCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTcgcg > 7:74886/1‑149 (MQ=255) gTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg < 1:39046/149‑1 (MQ=255) gcgAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCgg < 6:41953/149‑1 (MQ=255) aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg > 7:168293/1‑67 (MQ=255) aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg < 8:168293/67‑1 (MQ=255) gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg < 1:34423/121‑1 (MQ=255) gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg > 2:34423/1‑121 (MQ=255) gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt > 7:174657/1‑149 (MQ=255) ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc > 6:303802/1‑148 (MQ=255) ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc > 2:73713/1‑148 (MQ=255) ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg > 6:305066/1‑131 (MQ=25) ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg < 5:305066/131‑1 (MQ=25) gcgcCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa < 6:228359/149‑1 (MQ=255) ggggTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCcgcg > 8:184131/1‑149 (MQ=255) | GAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCG > NC_002947/1642126‑1642419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |