Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,642,285 G→C 12.7% intergenic (‑113/+39) PP_1442 ← / ← lon‑I hypothetical protein/DNA‑binding ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,642,2850GC12.7% 158.2 / 12.1 55intergenic (‑113/+39)PP_1442/lon‑Ihypothetical protein/DNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base C (3/4);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCGG  >  NC_002947/1642140‑1642431
                                                                                                                                                 |                                                                                                                                                   
gCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGc                                                                                                                                                  <  2:287792/149‑1 (MQ=21)
            gAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCtt                                                                                                                                     >  4:221572/1‑149 (MQ=255)
             aGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTg                                                                                                                                    >  7:245490/1‑149 (MQ=255)
                 gACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCcg                                                                                                                                >  7:134679/1‑149 (MQ=255)
                    gaggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGa                                                                                                                             <  7:87792/149‑1 (MQ=255)
                     aggagAGGTCATGGGGGCTCCGACGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGaa                                                                                                                            <  7:108130/149‑1 (MQ=255)
                         gagGGCATGGTGGCGCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGGCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAgg                                                                                                                        <  1:193322/149‑1 (MQ=255)
                            gTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCg                                                                                                                     >  4:70086/1‑149 (MQ=255)
                              cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg                                                                                                                                          >  8:51619/1‑126 (MQ=25)
                              cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg                                                                                                                                          <  7:51619/126‑1 (MQ=25)
                                  gtggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAg                                                                                                               >  1:163047/1‑149 (MQ=255)
                                      cTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa                                                                                                           <  2:163047/149‑1 (MQ=255)
                                         cGAAGGGTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTAGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAg                                                                                                          <  1:47278/148‑1 (MQ=255)
                                         cGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa                                                                                                        >  4:53548/1‑149 (MQ=255)
                                          gAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa                                                                                                        >  5:41953/1‑148 (MQ=255)
                                           aaGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa                                                                                                      <  8:227157/149‑1 (MQ=255)
                                            aGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAg                                                                                                     <  8:245490/149‑1 (MQ=255)
                                                tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg                                                                                                                    >  8:138543/1‑130 (MQ=255)
                                                tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg                                                                                                                    <  7:138543/130‑1 (MQ=255)
                                                  gCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACttt                                                                                               >  2:302450/1‑149 (MQ=255)
                                                    gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       <  2:293441/139‑1 (MQ=255)
                                                    gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       >  1:293441/1‑139 (MQ=255)
                                                      tGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGcc                                                                                           <  1:302450/149‑1 (MQ=255)
                                                             aGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCgg                                                                                    <  3:221572/149‑1 (MQ=255)
                                                                  aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg                                                                                                                                <  5:241526/100‑6 (MQ=17)
                                                                  aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg                                                                                                                                >  6:241526/1‑95 (MQ=17)
                                                                    ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGccc                                                                                                                                 <  6:71383/97‑1 (MQ=255)
                                                                    ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                                                              >  8:173535/1‑132 (MQ=255)
                                                                    ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                                                              <  7:173535/132‑1 (MQ=255)
                                                                     cGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       >  4:192668/1‑122 (MQ=255)
                                                                      gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc                                                                              <  1:30159/146‑1 (MQ=255)
                                                                      gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc                                                                              >  2:30159/1‑146 (MQ=255)
                                                                          atCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc                                                                       >  2:39046/1‑149 (MQ=255)
                                                                              aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGaaa                                                                   >  5:111526/1‑149 (MQ=255)
                                                                                          ctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAg                                                        <  3:70086/148‑1 (MQ=255)
                                                                                             ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGcc                                                     <  6:111526/148‑1 (MQ=255)
                                                                                             ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGc                                                      <  3:53548/147‑1 (MQ=255)
                                                                                             ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCt                                                    >  3:259339/1‑149 (MQ=255)
                                                                                                  gCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTcgcg                                               >  7:74886/1‑149 (MQ=255)
                                                                                                      gTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           <  1:39046/149‑1 (MQ=255)
                                                                                                              gcgAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCgg                                   <  6:41953/149‑1 (MQ=255)
                                                                                                                     aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg                                                                                                              <  8:168293/67‑1 (MQ=255)
                                                                                                                     aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg                                                                                                              >  7:168293/1‑67 (MQ=255)
                                                                                                                      gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg                                                       >  2:34423/1‑121 (MQ=255)
                                                                                                                      gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg                                                       <  1:34423/121‑1 (MQ=255)
                                                                                                                      gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt                           >  7:174657/1‑149 (MQ=255)
                                                                                                                        ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc                          >  2:73713/1‑148 (MQ=255)
                                                                                                                        ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc                          >  6:303802/1‑148 (MQ=255)
                                                                                                                        ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           >  6:305066/1‑131 (MQ=25)
                                                                                                                        ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           <  5:305066/131‑1 (MQ=25)
                                                                                                                          gcgcCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa                       <  6:228359/149‑1 (MQ=255)
                                                                                                                                   ggggTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCcgcg              >  8:184131/1‑149 (MQ=255)
                                                                                                                                       tAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTg          >  2:101476/1‑149 (MQ=255)
                                                                                                                                       tAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTg          <  4:259339/149‑1 (MQ=255)
                                                                                                                                               ccGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCgg  <  7:184131/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCGG  >  NC_002947/1642140‑1642431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑