Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,642,292 T→C 7.7% intergenic (‑120/+32) PP_1442 ← / ← lon‑I hypothetical protein/DNA‑binding ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,642,2920TC 7.7% 149.8 / 2.5 52intergenic (‑120/+32)PP_1442/lon‑Ihypothetical protein/DNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base C (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCGG  >  NC_002947/1642152‑1642431
                                                                                                                                            |                                                                                                                                            
gAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCtt                                                                                                                                     >  4:221572/1‑149 (MQ=255)
 aGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTg                                                                                                                                    >  7:245490/1‑149 (MQ=255)
     gACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCcg                                                                                                                                >  7:134679/1‑149 (MQ=255)
        gaggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGa                                                                                                                             <  7:87792/149‑1 (MQ=255)
         aggagAGGTCATGGGGGCTCCGACGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGaa                                                                                                                            <  7:108130/149‑1 (MQ=255)
             gagGGCATGGTGGCGCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGGCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAgg                                                                                                                        <  1:193322/149‑1 (MQ=255)
                gTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCg                                                                                                                     >  4:70086/1‑149 (MQ=255)
                  cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg                                                                                                                                          >  8:51619/1‑126 (MQ=25)
                  cATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGc‑gcg                                                                                                                                          <  7:51619/126‑1 (MQ=25)
                      gtggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAg                                                                                                               >  1:163047/1‑149 (MQ=255)
                          cTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa                                                                                                           <  2:163047/149‑1 (MQ=255)
                             cGAAGGGTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTAGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAg                                                                                                          <  1:47278/148‑1 (MQ=255)
                             cGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa                                                                                                        >  4:53548/1‑149 (MQ=255)
                              gAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa                                                                                                        >  5:41953/1‑148 (MQ=255)
                               aaGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa                                                                                                      <  8:227157/149‑1 (MQ=255)
                                aGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAg                                                                                                     <  8:245490/149‑1 (MQ=255)
                                    tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg                                                                                                                    >  8:138543/1‑130 (MQ=255)
                                    tGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCgg                                                                                                                    <  7:138543/130‑1 (MQ=255)
                                      gCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACttt                                                                                               >  2:302450/1‑149 (MQ=255)
                                        gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       <  2:293441/139‑1 (MQ=255)
                                        gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       >  1:293441/1‑139 (MQ=255)
                                          tGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGcc                                                                                           <  1:302450/149‑1 (MQ=255)
                                                 aGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCgg                                                                                    <  3:221572/149‑1 (MQ=255)
                                                      aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg                                                                                                                                >  6:241526/1‑95 (MQ=17)
                                                      aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCttactcg                                                                                                                                <  5:241526/100‑6 (MQ=17)
                                                        ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGccc                                                                                                                                 <  6:71383/97‑1 (MQ=255)
                                                        ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                                                              >  8:173535/1‑132 (MQ=255)
                                                        ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                                                              <  7:173535/132‑1 (MQ=255)
                                                         cGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAAc                                                                                                       >  4:192668/1‑122 (MQ=255)
                                                          gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc                                                                              >  2:30159/1‑146 (MQ=255)
                                                          gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgc                                                                              <  1:30159/146‑1 (MQ=255)
                                                              atCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc                                                                       >  2:39046/1‑149 (MQ=255)
                                                                  aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGaaa                                                                   >  5:111526/1‑149 (MQ=255)
                                                                              ctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAg                                                        <  3:70086/148‑1 (MQ=255)
                                                                                 ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGcc                                                     <  6:111526/148‑1 (MQ=255)
                                                                                 ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGc                                                      <  3:53548/147‑1 (MQ=255)
                                                                                 ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCt                                                    >  3:259339/1‑149 (MQ=255)
                                                                                      gCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTcgcg                                               >  7:74886/1‑149 (MQ=255)
                                                                                          gTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           <  1:39046/149‑1 (MQ=255)
                                                                                                  gcgAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCgg                                   <  6:41953/149‑1 (MQ=255)
                                                                                                         aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg                                                                                                              >  7:168293/1‑67 (MQ=255)
                                                                                                         aGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg                                                                                                              <  8:168293/67‑1 (MQ=255)
                                                                                                          gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg                                                       <  1:34423/121‑1 (MQ=255)
                                                                                                          gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAgg                                                       >  2:34423/1‑121 (MQ=255)
                                                                                                          gCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt                           >  7:174657/1‑149 (MQ=255)
                                                                                                            ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc                          >  6:303802/1‑148 (MQ=255)
                                                                                                            ccGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc                          >  2:73713/1‑148 (MQ=255)
                                                                                                            ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           >  6:305066/1‑131 (MQ=25)
                                                                                                            ccGCACCTGCACGGGTACGCTACCCCTGCAGGC‑GCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg                                           <  5:305066/131‑1 (MQ=25)
                                                                                                              gcgcCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa                       <  6:228359/149‑1 (MQ=255)
                                                                                                                       ggggTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCcgcg              >  8:184131/1‑149 (MQ=255)
                                                                                                                           tAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTg          >  2:101476/1‑149 (MQ=255)
                                                                                                                           tAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTg          <  4:259339/149‑1 (MQ=255)
                                                                                                                                   ccGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCgg  <  7:184131/149‑1 (MQ=255)
                                                                                                                                            t‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa                       >  2:146145/1‑119 (MQ=255)
                                                                                                                                            t‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa                       <  1:146145/119‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGT‑GCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGCCGCGGTTGGGCTCCGG  >  NC_002947/1642152‑1642431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑