Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,663,077 A→T 8.3% intergenic (+23/+15) purT → / ← yhjE phosphoribosylglycinamide formyltransferase/metabolite transport protein YhjE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,663,0770AT 8.3% 136.1 / 2.3 48intergenic (+23/+15)purT/yhjEphosphoribosylglycinamide formyltransferase/metabolite transport protein YhjE
Reads supporting (aligned to +/- strand):  ref base A (17/27);  new base T (2/2);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAAGCCGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGCTCAAACCCCCCATGCTCACCAGCTTCTGGGCAAAGAACGG  >  NC_002947/1662934‑1663220
                                                                                                                                               |                                                                                                                                               
cGGCAAGCCGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGc                                                                                                                                            >  3:250983/1‑149 (MQ=255)
    aaGCCGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGccc                                                                                                                                          >  1:104450/1‑147 (MQ=255)
      gCCGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGt                                                                                                                                      <  5:237495/149‑1 (MQ=255)
        cGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTgg                                                                                                                                    <  2:196788/149‑1 (MQ=255)
         ggAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCTCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGTGGCCGCTTTGCGGCCCCa                                                                                                                                        <  4:198658/144‑1 (MQ=255)
         ggAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCTTTGCGGCCCCa                                                                                                                                        >  3:198658/1‑144 (MQ=255)
              gcAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGGGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACa                                                                                                                              <  5:31552/148‑1 (MQ=255)
                aaTGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCTTTGCGGCCCCAgcact                                                                                                                                   >  7:129035/1‑138 (MQ=255)
                aaTGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCTTTGCGGCCCCAgcact                                                                                                                                   <  8:129035/142‑5 (MQ=255)
                  tGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGt                                                                                                                          >  5:250296/1‑149 (MQ=255)
                    gCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTg                                                                                                                         <  3:218378/148‑1 (MQ=255)
                      aCCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTa                                                                                                                      <  5:111459/149‑1 (MQ=255)
                         cgccgcATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTg                                                                                                                   <  7:161125/149‑1 (MQ=255)
                              cATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACa                                                                                                                              >  3:185775/1‑133 (MQ=255)
                              cATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACa                                                                                                                              <  4:185775/133‑1 (MQ=255)
                                     gtgtgCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTc                                                                                                       <  4:250983/149‑1 (MQ=255)
                                      tgtgCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCa                                                                                                      <  2:104450/149‑1 (MQ=255)
                                        tgCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTAtt                                                                                                                    <  7:287903/133‑1 (MQ=255)
                                        tgCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTAtt                                                                                                                    >  8:287903/1‑133 (MQ=255)
                                          ccTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAgg                                                                                                    <  4:248445/147‑1 (MQ=255)
                                          ccTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAgg                                                                                                    >  3:248445/1‑147 (MQ=255)
                                               cgcgTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCGTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCa                                                                                             <  8:113676/149‑1 (MQ=255)
                                                 cgTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGccc                                                                                                                                          <  8:67935/102‑1 (MQ=255)
                                                 cgTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGccc                                                                                                                                          >  7:67935/1‑102 (MQ=255)
                                                    gACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACg                                                                                           <  8:129967/146‑1 (MQ=255)
                                                    gACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACg                                                                                           >  7:129967/1‑146 (MQ=255)
                                                       gAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAAt                                                                                      >  8:52554/1‑148 (MQ=255)
                                                          tCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTgc                                                                                                                  >  7:14136/1‑117 (MQ=255)
                                                          tCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTgc                                                                                                                  <  8:14136/117‑1 (MQ=255)
                                                          tCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAgg                                                                                  >  3:142011/1‑149 (MQ=255)
                                                            ggTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCt                                                                                <  8:29066/149‑1 (MQ=255)
                                                                gAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAg                                                                                   <  1:134292/142‑1 (MQ=255)
                                                                gAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAg                                                                                   >  2:134292/1‑142 (MQ=255)
                                                                gAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGAt                                                                             <  5:74271/148‑1 (MQ=255)
                                                                gAACTGGCGAGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGAt                                                                             <  5:74269/148‑1 (MQ=255)
                                                                   cTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTg                                                                                                                         >  4:40616/1‑101 (MQ=255)
                                                                   cTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTg                                                                                                                         <  3:40616/101‑1 (MQ=255)
                                                                           ggCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCAc                                                                 <  4:142011/149‑1 (MQ=255)
                                                                             ccAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCg                                                               >  6:314210/1‑149 (MQ=255)
                                                                                  gCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGAcaca                                                          >  3:120597/1‑149 (MQ=255)
                                                                                   cGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACAt                                                         <  4:120597/149‑1 (MQ=255)
                                                                                       ccGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTACAGGCACAGCACGGCAATCCGGCTGATCACCGCCGCCCCCGACACAtgggc                                                     >  5:265237/1‑145 (MQ=255)
                                                                                                  aGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGc                                          <  5:314210/149‑1 (MQ=255)
                                                                                                       gTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGCTCaaa                                     <  7:52554/149‑1 (MQ=255)
                                                                                                                             agagTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAAccc                                                    <  6:32163/112‑1 (MQ=255)
                                                                                                                             agagTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAAccc                                                    >  5:32163/1‑112 (MQ=255)
                                                                                                                               agTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGCTCAAACCCCCCATGCTCACCAGCTTCTgg             <  3:199017/149‑1 (MQ=255)
                                                                                                                                          cGCAAAGCGGACCCAGTGGCTCACAGCGTGGTCTTGCGCGTCTCTTTCAGGCACAGCACCGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGCTCAAACCCCCCATGCTCACCAGCTTCTGGGCAAAGAACgg  <  8:275911/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CGGCAAGCCGGAAATCAATGGCACCCGCCGCATGGGTGTGTGCCTGGCGCGTGACGAATCGGTCGAACTGGCGCGGGCCAAGGCGACCCGCGCTTCGCAGGCGGTCAAGGTCGAGTTCTGATCCGAGAGTGCTGGGGCCGCAAAGCGGCCCCAGTGGCTTACAGCGTGGTATTGCGCGTCTCTTTCAGGCACAGCACGGCAATCAGGCTGATCACCGCCGCCACCGACACATAACCCCCCACCCAGCTCAAACCCCCCATGCTCACCAGCTTCTGGGCAAAGAACGG  >  NC_002947/1662934‑1663220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑