Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,667,060 A→G 7.3% K21R (AAG→AGG)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,667,0600AG 7.3% 157.7 / 2.7 55K21R (AAG→AGG) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base A (25/26);  new base G (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TCGCCGCCTTGCTGTGCCCGGCGGGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGCCTGA  >  NC_002947/1666914‑1667203
                                                                                                                                                  |                                                                                                                                               
tCGCCGCCTTGCTGTGCCCGGCGGGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCCCAGACGCTGCGCCATGTCACCGGCAAGGCCAAGc                                                                                                                                               >  1:131676/1‑149 (MQ=255)
tCGCCGCCTTGCTGTGCCCGGCGGGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGc                                                                                                                                               <  3:147130/149‑1 (MQ=255)
 cgccgcCTTGCTGTGCCCGGCGGGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCt                                                                                                                                              >  2:158424/1‑149 (MQ=255)
                    gcggGTGTGTGTTTCCCCCCTGGCACCTGCCAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAg                                                                                                                           <  5:283362/149‑1 (MQ=255)
                     cggGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAgg                                                                                                                          <  2:120393/149‑1 (MQ=255)
                       ggTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGac                                                                                                                        <  4:7675/149‑1 (MQ=255)
                         tgtgtgtTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAGGCTGACCGAAGACAACATCAAGGacac                                                                                                                      >  3:112370/1‑149 (MQ=255)
                               tttCCCCCCTGGCACCTGACAACCCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTgc                                                                                                                 <  6:267/148‑1 (MQ=255)
                                     cccTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGt                                                                                                          <  7:77952/149‑1 (MQ=255)
                                      ccTGGCACCTGACAAAGCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAGGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTg                                                                                                         >  5:67872/1‑149 (MQ=255)
                                          gCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGacaac                                                                                                                                 <  6:290821/121‑1 (MQ=255)
                                              cTGACAAACCTCGGCTTTACTGGGCCTGATAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGgc                                                                                                 >  8:192419/1‑149 (MQ=255)
                                                gACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAACCTGACCGAAGCCAACCACAAGGACACGCTGCGCGAAGTGCGGATgcggc                                                                                               >  8:288258/1‑145 (MQ=255)
                                                 aCAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGATAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGgcgc                                                                                               <  7:288258/148‑1 (MQ=255)
                                                   aaaCCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCgctg                                                                                             >  1:111342/1‑148 (MQ=255)
                                                     aCCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATAGCGCTGCTc                                                                                          <  1:158424/149‑1 (MQ=255)
                                                       cTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAg                                                                                                                                      >  6:318622/1‑103 (MQ=255)
                                                       cTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAg                                                                                                                                      <  5:318622/103‑1 (MQ=255)
                                                          ggCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGc                                                                                     >  8:129721/1‑149 (MQ=255)
                                                           gCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAATGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGcc                                                                                    <  6:146148/149‑1 (MQ=255)
                                                            cTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCg                                                                                   <  3:219126/149‑1 (MQ=255)
                                                                aCTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGt                                                                               >  1:274367/1‑149 (MQ=255)
                                                                    ggCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTg                                                                             <  5:52967/147‑1 (MQ=255)
                                                                          aaaGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGc                                                                       <  7:129721/147‑1 (MQ=255)
                                                                          aaaGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCg                                                                     >  5:288932/1‑149 (MQ=255)
                                                                              gATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAg                                                                 >  3:92503/1‑149 (MQ=255)
                                                                                tCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAGGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTc                                                               <  4:112370/149‑1 (MQ=255)
                                                                                tCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGt                                                                >  8:151511/1‑148 (MQ=255)
                                                                                   ccATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCgg                                                                    >  4:212831/1‑141 (MQ=255)
                                                                                   ccATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCgg                                                                    <  3:212831/141‑1 (MQ=255)
                                                                                   ccATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAg                                                            <  6:288932/149‑1 (MQ=255)
                                                                                        ttCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGAtt                                                        <  2:111342/148‑1 (MQ=255)
                                                                                          cGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTc                                                      <  2:147907/148‑1 (MQ=255)
                                                                                                 cTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAg                                                            >  1:154782/1‑135 (MQ=255)
                                                                                                 cTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAg                                                            <  2:154782/135‑1 (MQ=255)
                                                                                                       gaccgCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAGGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCaa                                        <  6:67872/149‑1 (MQ=255)
                                                                                                            ccTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATc                                   >  8:43472/1‑149 (MQ=255)
                                                                                                               gTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTgc                                                                                                        <  4:301841/77‑1 (MQ=255)
                                                                                                                 cacaGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCg                              <  4:92503/149‑1 (MQ=255)
                                                                                                                  acaGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCgg                             >  8:297733/1‑149 (MQ=255)
                                                                                                                    aGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTc                           >  8:43816/1‑149 (MQ=255)
                                                                                                                    aGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTc                           >  8:156870/1‑149 (MQ=255)
                                                                                                                    aGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTc                           <  7:192419/149‑1 (MQ=255)
                                                                                                                      aCGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGCCGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCgg                         >  3:143773/1‑149 (MQ=255)
                                                                                                                                   tCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTcg            >  8:93875/1‑149 (MQ=255)
                                                                                                                                   tCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTcg            >  5:69432/1‑149 (MQ=255)
                                                                                                                                    cACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTcgc           >  5:307210/1‑149 (MQ=255)
                                                                                                                                     aCCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTc             >  5:171342/1‑146 (MQ=255)
                                                                                                                                     aCCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTc             <  6:171342/146‑1 (MQ=255)
                                                                                                                                         gCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGc      <  7:156870/149‑1 (MQ=255)
                                                                                                                                         gCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGAGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCATCAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGc      >  6:169838/1‑149 (MQ=255)
                                                                                                                                          cAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGcc     <  7:93875/149‑1 (MQ=255)
                                                                                                                                           aaGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGCCt    <  2:274367/149‑1 (MQ=255)
                                                                                                                                           aaGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGCCt    >  5:78297/1‑149 (MQ=255)
                                                                                                                                             ggCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGCCTGa  >  3:108611/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCGCCGCCTTGCTGTGCCCGGCGGGTGTGTGTTTCCCCCCTGGCACCTGACAAACCTCGGCTTTACTGGGCCTGAAAGGATCACCATGTTCGAAAACCTGACCGACCGCCTGTCACAGACGCTGCGCCATGTCACCGGCAAGGCCAAGCTGACCGAAGACAACATCAAGGACACGCTGCGCGAAGTGCGCATGGCGCTGCTCGAGGCCGACGTTGCCCTGCCGGTAGTCAAGGATTTCGTCAACAGCGTCAAGGATCGTGCGGTCGGGACCGAGGTGTCGCGCAGCCTGA  >  NC_002947/1666914‑1667203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑