Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,698,641 T→C 10.0% V130A (GTC→GCC)  cheBC → chemotaxis response regulator protein‑glutamate methylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,698,6410TC10.0% 100.0 / 3.4 41V130A (GTC→GCC) cheBCchemotaxis response regulator protein‑glutamate methylesterase
Reads supporting (aligned to +/- strand):  ref base T (18/18);  new base C (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CCGTAAACAGAACGTCCACCGGGTATTCGAGGCCATGGGCCATGGCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGCCGGCAGCGTT  >  NC_002947/1698498‑1698785
                                                                                                                                               |                                                                                                                                                 
ccGTAAACAGAACGTCCACCGGGTATTCGAGGCCATGGGCCATGGCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGcc                                                                                                                                              <  7:287335/149‑1 (MQ=255)
                         ttCGAGGCCATGGGCCATGGCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGt                                                                                                                     <  5:175877/148‑1 (MQ=255)
                                 catgggccatgtCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTg                                                                                                            <  8:20678/149‑1 (MQ=255)
                                 catgggccatggCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCCGACcagccag‑ccga                                                                                                                                       >  4:237884/1‑119 (MQ=255)
                                 catgggccatggCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCCGACcagccag‑ccga                                                                                                                                       <  3:237884/122‑4 (MQ=255)
                                  atgggccatggCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTg                                                                                                            >  4:47454/1‑148 (MQ=255)
                                  atgggccatggCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGc                                                                                                           >  3:194005/1‑149 (MQ=255)
                                              gcCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTgcgc                                                                                                 >  2:14097/1‑147 (MQ=255)
                                                      tgtgGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTcc                                                                                        >  8:39356/1‑148 (MQ=255)
                                                      tgtgGTCGACACACCTGCGATGGGCGCTGGCGATGCCCGCGAAGCGCCGGCCCCCTTCCAGCCCAAGATCCTCAATATCGGCTCGCTGGTCGGCCAG‑CAGCGTGCAACTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCATcc                                                                                        >  4:239625/1‑148 (MQ=255)
                                                        tgGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTg                                                                                                                                    <  1:277596/102‑1 (MQ=255)
                                                        tgGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTg                                                                                                                                    >  2:277596/1‑102 (MQ=255)
                                                                 ccccTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCggg                                                                            <  7:219787/149‑1 (MQ=255)
                                                                  cccTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGt                                                                           >  6:118820/1‑149 (MQ=255)
                                                                  cccTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGt                                                                           >  2:270582/1‑149 (MQ=255)
                                                                  cccTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGt                                                                           >  5:105863/1‑149 (MQ=255)
                                                                        gTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTtggtgg                                                                     <  1:14097/149‑1 (MQ=255)
                                                                                          ccGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTcggcgg                                                   >  3:213734/1‑149 (MQ=255)
                                                                                           cgcgAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGTAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCggg                                                  >  2:99117/1‑149 (MQ=255)
                                                                                                        cgcCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGgcagca                                     <  5:118820/149‑1 (MQ=255)
                                                                                                               gctgcGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGa                                <  3:47454/147‑1 (MQ=255)
                                                                                                                   cgcAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGtgc                           <  1:153788/148‑1 (MQ=255)
                                                                                                                    gcAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGGTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGtgcgg                         >  4:239484/1‑147 (MQ=255)
                                                                                                                      aaGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGTAAGGTCGGTGGCTGCACCGCTCCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTTCGAGtgctgcc                       <  1:99117/149‑2 (MQ=255)
                                                                                                                      aaGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTTCGAGtgctgcc                       <  8:146612/149‑2 (MQ=255)
                                                                                                                          tCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGgca                                        >  6:316383/1‑128 (MQ=255)
                                                                                                                          tCCTCAATATCGGCTGGCTGGGCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGGCTTCGGCGGGTGGCCCGgca                                        <  5:316383/128‑1 (MQ=255)
                                                                                                                           ccTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAgg                  >  7:230323/1‑149 (MQ=255)
                                                                                                                            cTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGGAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTTCTCAAgg                  <  3:239625/148‑1 (MQ=255)
                                                                                                                            cTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACGGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGTGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAgg                  <  7:39356/148‑1 (MQ=255)
                                                                                                                            cTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAggg                 >  3:218549/1‑149 (MQ=255)
                                                                                                                             tCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGt                >  8:220178/1‑149 (MQ=255)
                                                                                                                               aaTATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGttt              >  6:99866/1‑149 (MQ=255)
                                                                                                                               aaTATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGttt              <  6:105863/149‑1 (MQ=255)
                                                                                                                               aaTATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGttt              <  4:218549/149‑1 (MQ=255)
                                                                                                                                 tatCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGc            <  4:213734/149‑1 (MQ=255)
                                                                                                                                   tCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGCCg          <  7:220178/149‑1 (MQ=255)
                                                                                                                                     ggctggctGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGCCGGc        <  8:230323/149‑1 (MQ=255)
                                                                                                                                     acgctgctggCCGACCAGCCAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGaa                               >  4:159905/6‑127 (MQ=255)
                                                                                                                                     acgctgctggCCGACCAGCCAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGaa                               <  3:159905/122‑1 (MQ=255)
                                                                                                                                           ctggTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGCCGGCAGCGtt  >  4:253849/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCGTAAACAGAACGTCCACCGGGTATTCGAGGCCATGGGCCATGGCGCCCTCGATGTGGTCGACACCCCTGCGTTGGGCGCTGGCGATGCCCGCGAAGCGGCGGCGCCCTTGCTGCGCAAGATCCTCAATATCGGCTGGCTGGTCGGCCAG‑CAGCGTGCACCTGCCGCAAGGTCGGTGGCTGCACCGCTGCGCGAGGCTTCCCAGCGCCGCGGGTTGGTGGCAATCGGGTCTTCGGCGGGTGGCCCGGCAGCACTCGAAGTGCTGCTCAAGGGTTTGCCGGCAGCGTT  >  NC_002947/1698498‑1698785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑