Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,703,221	T→C	16.2%	intergenic (+70/‑97)	lysS → / → PP_1497	lysine‑‑tRNA ligase/TetR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,703,221	0	T	C	16.2%	96.5 / 9.4	44	intergenic (+70/‑97)	lysS/PP_1497	lysine‑‑tRNA ligase/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (16/20);  new base C (3/4);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGT  >  NC_002947/1703076‑1703367                                                                                                                                                  |                                                                                                                                                   ccTGGTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCtt                                                                                                                                                 >  5:9451/1‑149 (MQ=255)     gTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTcgc                                                                                                                                              <  3:168503/148‑1 (MQ=255)      tGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGGCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCgg                                                                                                                                            <  8:175256/149‑1 (MQ=255)      tGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCgg                                                                                                                                            <  4:292667/149‑1 (MQ=255)        aTGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCggg                                                                                                                                           >  1:186858/1‑148 (MQ=255)        aTGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGt                                                                                                                                          >  3:168773/1‑149 (MQ=255)                    aaCTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCt                                                                                                                              <  7:38953/149‑1 (MQ=255)                             tCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGcc                                                                                                                     <  3:167530/149‑1 (MQ=255)                               gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                   >  6:183816/1‑149 (MQ=255)                               gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                   <  5:135501/149‑1 (MQ=255)                                aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTGAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg                                                                                                                  <  2:117202/149‑1 (MQ=255)                                 tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCtttg                                                                                                                 >  3:135716/1‑146 (MQ=38)                                   cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAg                                                                                                               <  1:134073/149‑1 (MQ=38)                                   cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTa                                                                                                                <  5:183816/148‑1 (MQ=255)                                      gACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAg                                                                                                            <  4:135716/149‑1 (MQ=37)                                       aCGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                           <  6:9451/149‑1 (MQ=255)                                         gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                         <  2:186858/149‑1 (MQ=255)                                         gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                         <  3:109098/149‑1 (MQ=255)                                         gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCCTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                         <  4:35798/149‑1 (MQ=255)                                            aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGtt                                                                                                      >  1:46314/1‑149 (MQ=255)                                            aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAAACCCCTCCGACGAAGGGCATGTAGGAGCggggt                                                                                                      >  1:290416/1‑147 (MQ=255)                                                  ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                         >  4:150419/1‑140 (MQ=255)                                                  ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                         <  3:150419/140‑1 (MQ=255)                                                                tCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCgg                                                                                                                                            >  4:233477/1‑91 (MQ=255)                                                                tCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCgg                                                                                                                                            <  3:233477/91‑1 (MQ=255)                                                                 ccGCTGGCCTGCGTGCCCTCACCCCGCCGCCTTGCGCGGGGGTTTTTGCTTGCCTCAAGATTTATTTGGCCATTCCCGCCCTCTGCGCGGTAAACCCCGCCCCAACAACGCCGTTTTCGGCGGGGGTTAACCCGCGAAGGGGCCAGCAc                                                                                 <  2:46314/149‑1 (MQ=255)                                                                 ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                 >  7:66913/1‑117 (MQ=255)                                                                 ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                 <  8:66913/117‑1 (MQ=255)                                                                                   tGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAt                                                                >  5:101697/1‑148 (MQ=255)                                                                                    gAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCg                                                                                                                                   >  4:52320/1‑80 (MQ=37)                                                                                    gAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCg                                                                                                                                   <  3:52320/80‑1 (MQ=37)                                                                                          gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                             >  6:153607/1‑96 (MQ=255)                                                                                          gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                             <  5:153607/96‑1 (MQ=255)                                                                                                         ggcTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCGACAAAGGCCTTGTATGAGCGGGTTTAACCGCGAATGGGGCAGCCCTGACGAAACCCCCCCATGAGGGATCCCCCGTGACCCCCGc                                         >  5:2152/1‑149 (MQ=37)                                                                                                           cTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCCAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGGGCCACCCGCCa                                       >  5:324378/1‑149 (MQ=255)                                                                                                            tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCa                                                                                  >  7:131777/1‑105 (MQ=255)                                                                                                            tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                           <  7:117343/80‑1 (MQ=18)                                                                                                            tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                           >  8:117343/1‑80 (MQ=18)                                                                                                             ttttCTTGCCTCAAGTTTTTTGTTGGACGTCCCGGCCTATTCGCGGGTAAACCCGCTCCTACAATGGCTTTGTCGGTGGGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt                                      <  6:101697/148‑1 (MQ=25)                                                                                                                tCTTGCCTTATGCTTTATGTTGTCTGTACCGGCCTCTTCGCGTTTAACCCCGCTCCTACAAAGGGCTTGTTGGTGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGcc                                   <  6:324378/148‑1 (MQ=37)                                                                                                                     ccTTAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAAAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGAcc                              <  7:95729/148‑1 (MQ=255)                                                                                                                                           cccGGCCGCTTCCCGGGTAACCCCGCCCCAACAACGGCCTTGTAGGAGCGGGTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAcc       <  7:31175/148‑1 (MQ=255)                                                                                                                                                ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt  >  5:64960/1‑149 (MQ=255)                                                                                                                                                ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt  >  2:211958/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CCTGGTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGT  >  NC_002947/1703076‑1703367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑