Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,703,248	A→G	32.0%	intergenic (+97/‑70)	lysS → / → PP_1497	lysine‑‑tRNA ligase/TetR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,703,248	0	A	G	32.0%	42.1 / 31.3	43	intergenic (+97/‑70)	lysS/PP_1497	lysine‑‑tRNA ligase/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (16/11);  new base G (6/7);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

TCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCG  >  NC_002947/1703104‑1703394                                                                                                                                                 |                                                                                                                                                   tCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGcc                                                                                                                                                <  3:167530/149‑1 (MQ=255)   gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                                              <  5:135501/149‑1 (MQ=255)   gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                                              >  6:183816/1‑149 (MQ=255)    aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTGAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg                                                                                                                                             <  2:117202/149‑1 (MQ=255)     tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCtttg                                                                                                                                            >  3:135716/1‑146 (MQ=38)       cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAg                                                                                                                                          <  1:134073/149‑1 (MQ=38)       cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTa                                                                                                                                           <  5:183816/148‑1 (MQ=255)          gACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAg                                                                                                                                       <  4:135716/149‑1 (MQ=37)           aCGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                      <  6:9451/149‑1 (MQ=255)             gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                                                    <  2:186858/149‑1 (MQ=255)             gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                    <  3:109098/149‑1 (MQ=255)             gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCCTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                                                    <  4:35798/149‑1 (MQ=255)                aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGtt                                                                                                                                 >  1:46314/1‑149 (MQ=255)                aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAAACCCCTCCGACGAAGGGCATGTAGGAGCggggt                                                                                                                                 >  1:290416/1‑147 (MQ=255)                      ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                    <  3:150419/140‑1 (MQ=255)                      ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                    >  4:150419/1‑140 (MQ=255)                                     ccGCTGGCCTGCGTGCCCTCACCCCGCCGCCTTGCGCGGGGGTTTTTGCTTGCCTCAAGATTTATTTGGCCATTCCCGCCCTCTGCGCGGTAAACCCCGCCCCAACAACGCCGTTTTCGGCGGGGGTTAACCCGCGAAGGGGCCAGCAc                                                                                                            <  2:46314/149‑1 (MQ=255)                                     ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                                            <  8:66913/117‑1 (MQ=255)                                     ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                                            >  7:66913/1‑117 (MQ=255)                                                       tGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAt                                                                                           >  5:101697/1‑148 (MQ=255)                                                              gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                                                        <  5:153607/96‑1 (MQ=255)                                                              gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                                                        >  6:153607/1‑96 (MQ=255)                                                                             ggcTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCGACAAAGGCCTTGTATGAGCGGGTTTAACCGCGAATGGGGCAGCCCTGACGAAACCCCCCCATGAGGGATCCCCCGTGACCCCCGc                                                                    >  5:2152/1‑149 (MQ=37)                                                                               cTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCCAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGGGCCACCCGCCa                                                                  >  5:324378/1‑149 (MQ=255)                                                                                tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCa                                                                                                             >  7:131777/1‑105 (MQ=255)                                                                                tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                      >  8:117343/1‑80 (MQ=18)                                                                                tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                      <  7:117343/80‑1 (MQ=18)                                                                                 ttttCTTGCCTCAAGTTTTTTGTTGGACGTCCCGGCCTATTCGCGGGTAAACCCGCTCCTACAATGGCTTTGTCGGTGGGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt                                                                 <  6:101697/148‑1 (MQ=25)                                                                                    tCTTGCCTTATGCTTTATGTTGTCTGTACCGGCCTCTTCGCGTTTAACCCCGCTCCTACAAAGGGCTTGTTGGTGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGcc                                                              <  6:324378/148‑1 (MQ=37)                                                                                         ccTTAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAAAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGAcc                                                         <  7:95729/148‑1 (MQ=255)                                                                                                               cccGGCCGCTTCCCGGGTAACCCCGCCCCAACAACGGCCTTGTAGGAGCGGGTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAcc                                  <  7:31175/148‑1 (MQ=255)                                                                                                                    ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt                             >  5:64960/1‑149 (MQ=255)                                                                                                                    ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt                             >  2:211958/1‑149 (MQ=255)                                                                                                                          cgcgGGTTAACCCGCAACTACAAAGGCCATGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAAAACCCCCATGAGGTATACCCCCTGACACCCGCCAAGGCGGACCAACGCGCCGCCGGCAAAGCCACCGCCGTCGCCg                        >  8:193887/1‑148 (MQ=37)                                                                                                                             gggTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAg                    >  3:281763/1‑149 (MQ=255)                                                                                                                                  aaCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc               <  1:211958/149‑1 (MQ=255)                                                                                                                                   aCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGa                                                                           >  4:181284/1‑88 (MQ=255)                                                                                                                                   aCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGa                                                                           <  3:181284/88‑1 (MQ=255)                                                                                                                                     ccGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCC‑TCGCCGAAAGCGTGCAATa           >  1:149926/1‑149 (MQ=255)                                                                                                                                      cGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATa           >  1:115192/1‑149 (MQ=255)                                                                                                                                       gCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc          >  7:266168/1‑149 (MQ=255)                                                                                                                                       gCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc          >  3:31983/1‑149 (MQ=255)                                                                                                                                           cTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAg      >  1:296530/1‑149 (MQ=255)                                                                                                                                               aaaGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCg  >  7:220573/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCG  >  NC_002947/1703104‑1703394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑