Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,703,252 C→T 33.2% intergenic (+101/‑66) lysS → / → PP_1497 lysine‑‑tRNA ligase/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,703,2520CT33.2% 41.2 / 31.0 43intergenic (+101/‑66)lysS/PP_1497lysine‑‑tRNA ligase/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/11);  new base T (6/8);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-02

TCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGA  >  NC_002947/1703104‑1703399
                                                                                                                                                    |                                                                                                                                                   
tCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGcc                                                                                                                                                     <  3:167530/149‑1 (MQ=255)
  gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                                                   <  5:135501/149‑1 (MQ=255)
  gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt                                                                                                                                                   >  6:183816/1‑149 (MQ=255)
   aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTGAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg                                                                                                                                                  <  2:117202/149‑1 (MQ=255)
    tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCtttg                                                                                                                                                 >  3:135716/1‑146 (MQ=38)
      cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAg                                                                                                                                               <  1:134073/149‑1 (MQ=38)
      cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTa                                                                                                                                                <  5:183816/148‑1 (MQ=255)
         gACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAg                                                                                                                                            <  4:135716/149‑1 (MQ=37)
          aCGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                           <  6:9451/149‑1 (MQ=255)
            gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                                                         <  2:186858/149‑1 (MQ=255)
            gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                         <  3:109098/149‑1 (MQ=255)
            gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCCTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCgg                                                                                                                                         <  4:35798/149‑1 (MQ=255)
               aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGtt                                                                                                                                      >  1:46314/1‑149 (MQ=255)
               aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAAACCCCTCCGACGAAGGGCATGTAGGAGCggggt                                                                                                                                      >  1:290416/1‑147 (MQ=255)
                     ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                         <  3:150419/140‑1 (MQ=255)
                     ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg                                                                                                                                         >  4:150419/1‑140 (MQ=255)
                                    ccGCTGGCCTGCGTGCCCTCACCCCGCCGCCTTGCGCGGGGGTTTTTGCTTGCCTCAAGATTTATTTGGCCATTCCCGCCCTCTGCGCGGTAAACCCCGCCCCAACAACGCCGTTTTCGGCGGGGGTTAACCCGCGAAGGGGCCAGCAc                                                                                                                 <  2:46314/149‑1 (MQ=255)
                                    ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                                                 <  8:66913/117‑1 (MQ=255)
                                    ccGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt                                                                                                                                                 >  7:66913/1‑117 (MQ=255)
                                                      tGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAt                                                                                                >  5:101697/1‑148 (MQ=255)
                                                             gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                                                             <  5:153607/96‑1 (MQ=255)
                                                             gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGa                                                                                                                                             >  6:153607/1‑96 (MQ=255)
                                                                            ggcTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCGACAAAGGCCTTGTATGAGCGGGTTTAACCGCGAATGGGGCAGCCCTGACGAAACCCCCCCATGAGGGATCCCCCGTGACCCCCGc                                                                         >  5:2152/1‑149 (MQ=37)
                                                                              cTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCCAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGGGCCACCCGCCa                                                                       >  5:324378/1‑149 (MQ=255)
                                                                               tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCa                                                                                                                  >  7:131777/1‑105 (MQ=255)
                                                                               tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                           <  7:117343/80‑1 (MQ=18)
                                                                               tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc                                                                                                                                           >  8:117343/1‑80 (MQ=18)
                                                                                ttttCTTGCCTCAAGTTTTTTGTTGGACGTCCCGGCCTATTCGCGGGTAAACCCGCTCCTACAATGGCTTTGTCGGTGGGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt                                                                      <  6:101697/148‑1 (MQ=25)
                                                                                   tCTTGCCTTATGCTTTATGTTGTCTGTACCGGCCTCTTCGCGTTTAACCCCGCTCCTACAAAGGGCTTGTTGGTGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGcc                                                                   <  6:324378/148‑1 (MQ=37)
                                                                                        ccTTAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAAAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGAcc                                                              <  7:95729/148‑1 (MQ=255)
                                                                                                              cccGGCCGCTTCCCGGGTAACCCCGCCCCAACAACGGCCTTGTAGGAGCGGGTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAcc                                       <  7:31175/148‑1 (MQ=255)
                                                                                                                   ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACCCCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt                                  >  5:64960/1‑149 (MQ=255)
                                                                                                                   ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt                                  >  2:211958/1‑149 (MQ=255)
                                                                                                                         cgcgGGTTAACCCGCAACTACAAAGGCCATGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAAAACCCCCATGAGGTATACCCCCTGACACCCGCCAAGGCGGACCAACGCGCCGCCGGCAAAGCCACCGCCGTCGCCg                             >  8:193887/1‑148 (MQ=37)
                                                                                                                            gggTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAg                         >  3:281763/1‑149 (MQ=255)
                                                                                                                                 aaCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc                    <  1:211958/149‑1 (MQ=255)
                                                                                                                                  aCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGa                                                                                <  3:181284/88‑1 (MQ=255)
                                                                                                                                  aCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGa                                                                                >  4:181284/1‑88 (MQ=255)
                                                                                                                                    ccGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCC‑TCGCCGAAAGCGTGCAATa                >  1:149926/1‑149 (MQ=255)
                                                                                                                                     cGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATa                >  1:115192/1‑149 (MQ=255)
                                                                                                                                      gCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc               >  7:266168/1‑149 (MQ=255)
                                                                                                                                      gCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc               >  3:31983/1‑149 (MQ=255)
                                                                                                                                          cTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAg           >  1:296530/1‑149 (MQ=255)
                                                                                                                                              aaaGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCg       >  7:220573/1‑149 (MQ=255)
                                                                                                                                                   ccTTGTAGGAGGGGTTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGa  <  7:193887/149‑1 (MQ=255)
                                                                                                                                                   ccTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGa  <  6:108897/149‑1 (MQ=255)
                                                                                                                                                   ccTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGa  <  4:281763/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGA  >  NC_002947/1703104‑1703399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑