Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,738,930 A→T 5.1% V88D (GTC→GAC)  PP_1532 ← putative Phage integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,738,9300AT 5.1% 240.4 / 2.2 78V88D (GTC→GAC) PP_1532putative Phage integrase
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base T (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GGTCGATAGGTAATCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCAAACATCC  >  NC_002947/1738782‑1739077
                                                                                                                                                    |                                                                                                                                                    
ggTCGATAGGTAATCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTcga                                                                                                                                                      <  2:307559/149‑1 (MQ=255)
    gATAGGTAATCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGCCCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGCTTCGGAAAGCTCACGGGCCTTCAGGGCTGCAAAGTACTAGACGAt                                                                                                                                                  >  8:276086/1‑149 (MQ=255)
     aTAGGTAATCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATc                                                                                                                                                 <  8:98193/149‑1 (MQ=255)
             tCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCt                                                                                                                                         <  3:298042/149‑1 (MQ=255)
             tCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGCTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCt                                                                                                                                         <  7:166185/149‑1 (MQ=255)
                       ccATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGa                                                                                                                               >  8:166135/1‑149 (MQ=255)
                        cATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGa                                                                                                                               <  2:108593/148‑1 (MQ=255)
                          tGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCa                                                                                                                            <  1:306416/149‑1 (MQ=255)
                           ggTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAg                                                                                                                           >  8:199829/1‑149 (MQ=255)
                             tcgtcAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGTCGATCCACTCGCTGAATTTCCGATCAGgc                                                                                                                         >  8:81630/1‑149 (MQ=255)
                             tcgtcAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGTCGATCCACTCGCTGAATTTCCGATCAGgc                                                                                                                         >  8:77232/1‑149 (MQ=255)
                                        cGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGtt                                                                                                               <  5:225640/148‑1 (MQ=255)
                                         ggATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCg                                                                                                             >  8:272461/1‑149 (MQ=255)
                                         ggATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGAGCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCg                                                                                                             >  2:262242/1‑149 (MQ=255)
                                             aCCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGAATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGa                                                                                                                                       <  3:63168/119‑1 (MQ=255)
                                             aCCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGAATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGa                                                                                                                                       >  4:63168/1‑119 (MQ=255)
                                                 cgcTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTAt                                                                                                    >  8:120048/1‑149 (MQ=255)
                                                 cgcTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTAt                                                                                                    <  3:269307/149‑1 (MQ=255)
                                                       ccGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATgcgcgc                                                                                              >  2:4608/1‑149 (MQ=255)
                                                        cGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTc                                                                                                              >  5:220981/1‑133 (MQ=255)
                                                        cGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTc                                                                                                              <  6:220981/133‑1 (MQ=255)
                                                        cGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATgcgcg                                                                                               <  7:120048/147‑1 (MQ=255)
                                                        cGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATgcgc                                                                                                <  7:199829/146‑1 (MQ=255)
                                                           aTGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGAc                                                                                          <  1:24343/149‑1 (MQ=255)
                                                            tGCGTTCTCGCCCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGACAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACa                                                                                         <  7:276086/149‑1 (MQ=255)
                                                                 tctcGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGt                                                                                     <  7:166135/148‑1 (MQ=255)
                                                                   tcGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTggg                                                                                  >  3:269417/1‑149 (MQ=255)
                                                                     gACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATgcgcgc                                                                                              >  2:51434/1‑135 (MQ=255)
                                                                     gACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATgcgcgc                                                                                              <  1:51434/135‑1 (MQ=255)
                                                                     gACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGggcg                                                                                >  6:22954/1‑149 (MQ=255)
                                                                       cccGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGggcggc                                                                              <  4:268081/149‑1 (MQ=255)
                                                                       cccGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGggcggc                                                                              <  5:22954/149‑1 (MQ=255)
                                                                           gTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGa                                                                          >  8:246559/1‑149 (MQ=255)
                                                                                gTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGc                                                                     <  7:272461/149‑1 (MQ=255)
                                                                                 tCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGgcgcgc                                                                                                                     >  3:142404/1‑101 (MQ=255)
                                                                                 tCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGgcgcgc                                                                                                                     <  4:142404/101‑1 (MQ=255)
                                                                                   gCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAg                                                                   <  2:24877/148‑1 (MQ=255)
                                                                                          aaGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGt                                                           >  3:71434/1‑149 (MQ=255)
                                                                                          aaGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGt                                                           >  4:118382/1‑149 (MQ=255)
                                                                                          aaGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGt                                                           >  3:121730/1‑149 (MQ=255)
                                                                                                gcgcATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCg                                                      <  7:246559/148‑1 (MQ=255)
                                                                                                 cgcATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCg                                                      >  7:295903/1‑147 (MQ=255)
                                                                                                    aTGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGCCGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGAc                                                 >  7:195409/1‑149 (MQ=255)
                                                                                                       cTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGc                                              <  8:295903/149‑1 (MQ=255)
                                                                                                        tCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAgg                                                                  >  4:272814/1‑128 (MQ=255)
                                                                                                        tCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAgg                                                                  <  3:272814/128‑1 (MQ=255)
                                                                                                          gATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCg                                               <  2:117747/145‑1 (MQ=255)
                                                                                                          gATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCAGATCAGGCGCGATCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGcag                                            >  1:117747/1‑146 (MQ=255)
                                                                                                           aTTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCa                                          <  4:71434/149‑1 (MQ=255)
                                                                                                           aTTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCa                                          <  4:121730/149‑1 (MQ=255)
                                                                                                            ttCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGTCGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAg                                         <  7:77232/149‑1 (MQ=255)
                                                                                                            ttCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGTCGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAg                                         <  7:81630/149‑1 (MQ=255)
                                                                                                            ttCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCCCTCGCTGAATTTCCGATCAGGCGCGCGCGGTTCGGGCGAATATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCa                                          <  1:248924/149‑1 (MQ=255)
                                                                                                            ttCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAg                                         <  3:118382/149‑1 (MQ=255)
                                                                                                               tGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGa                                      >  4:180987/1‑149 (MQ=255)
                                                                                                                gAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGAt                                     >  1:191355/1‑149 (MQ=255)
                                                                                                                 aaaGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGc                                              <  4:51732/139‑1 (MQ=255)
                                                                                                                 aaaGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGc                                              >  3:51732/1‑139 (MQ=255)
                                                                                                                    gCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCt                                 >  4:1945/1‑149 (MQ=255)
                                                                                                                    gCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCt                                 >  2:53078/1‑149 (MQ=255)
                                                                                                                       cACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGgcgc                                                                            >  1:130614/1‑103 (MQ=255)
                                                                                                                       cACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGgcgc                                                                            <  2:130614/103‑1 (MQ=255)
                                                                                                                        aCGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTg                             >  5:44045/1‑149 (MQ=255)
                                                                                                                          ggTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGcc                           <  4:269417/149‑1 (MQ=255)
                                                                                                                            tCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTg                                                                                    >  8:221284/1‑90 (MQ=255)
                                                                                                                              cTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGt                       >  2:113331/1‑149 (MQ=255)
                                                                                                                               ttCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTg                        <  7:280636/147‑1 (MQ=255)
                                                                                                                               ttCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTg                        >  8:280636/1‑147 (MQ=255)
                                                                                                                               ttCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTc                      >  3:288947/1‑149 (MQ=255)
                                                                                                                               ttCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTc                      >  3:284574/1‑149 (MQ=255)
                                                                                                                                    gTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGt                                                           <  4:128493/107‑1 (MQ=255)
                                                                                                                                    gTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGt                                                           >  3:128493/1‑107 (MQ=255)
                                                                                                                                        gCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAg             >  2:280800/1‑149 (MQ=255)
                                                                                                                                         cAAAGTACTCGACGATCCACTCGCTGAATTTCTGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAgg            <  2:192289/149‑1 (MQ=255)
                                                                                                                                         cAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCGGCAGGGCCATGTTGGCGGAGCCCGGCTCCGCGCGGGCTTTTGCGGTGGCGCTGCGCAgg            >  4:255961/1‑149 (MQ=255)
                                                                                                                                          aaaGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTATGCCTTGTCGCTGCCCAGGc           >  7:13549/1‑149 (MQ=255)
                                                                                                                                             gTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCaa        <  1:4608/149‑1 (MQ=255)
                                                                                                                                                cTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCAAAc      <  3:255961/148‑1 (MQ=255)
                                                                                                                                                cTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCAAAc      >  7:201480/1‑148 (MQ=255)
                                                                                                                                                   gacgaTCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCAAACATcc  >  4:144333/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTCGATAGGTAATCCGCGATGTCCATGGTCGTCAGCGTGCGGATACCGCGCTCTCCGAATGCTTTCTCGACCCGGTTCAGTCGCATACGAAGGTTGCGCATGCTCGATTCTGAAAGCTCACGGTCCTTCAGGTCTGCAAAGTACTCGACGATCCACTCGCTGAATTTCCGATCAGGCGCGCTCGGTTCGGGCG‑CTATGCGCGCTGACAGGGTGGGCGGCGCGACCTGCAGGGCCATGTTGGCGGAGACCGCCTCAGCGATCGCTTTTGCCTTGTCGCTGCCCAGGCCAAACATCC  >  NC_002947/1738782‑1739077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑