Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,780,737 T→A 8.1% I203F (ATC→TTC)  dapC ← N‑succinyl‑L,L‑diaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,780,7370TA 8.1% 145.1 / 3.4 49I203F (ATC→TTC) dapCN‑succinyl‑L,L‑diaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGG  >  NC_002947/1780596‑1780882
                                                                                                                                             |                                                                                                                                                 
ggTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGTAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAg                                                                                                                                            >  6:71038/1‑149 (MQ=255)
ggTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAg                                                                                                                                            <  1:18618/149‑1 (MQ=255)
        cgcTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTc                                                                                                                                    <  4:170824/149‑1 (MQ=255)
         gcTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAt                                                                                                                                                   <  4:51857/133‑1 (MQ=255)
         gcTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAt                                                                                                                                                   >  3:51857/1‑133 (MQ=255)
             ggACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCa                                                                                                                                <  4:95448/148‑1 (MQ=255)
                   gCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAggg                                                                                                                         >  4:253658/1‑149 (MQ=255)
                     tgtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGc                                                                                                                        >  4:144990/1‑148 (MQ=255)
                      gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                 >  7:281486/1‑136 (MQ=255)
                      gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                 <  8:281486/138‑3 (MQ=255)
                        ggAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCATTc                                                                                                                    >  6:105315/1‑149 (MQ=255)
                           acacCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGc                                                                                                                 <  7:242589/149‑1 (MQ=255)
                                 cGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGaa                                                                                                                                             <  6:208414/115‑1 (MQ=255)
                                 cGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGaa                                                                                                                                             >  5:208414/1‑115 (MQ=255)
                                         gcAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGAGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                    >  7:219701/1‑148 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                        >  6:247765/1‑131 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                        <  5:247765/131‑1 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAgc                                                                                      <  6:326749/149‑1 (MQ=255)
                                                        ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCa                                                                                                                   <  3:264290/118‑1 (MQ=255)
                                                        ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGATCGTCAGCCAGGGCAATCa                                                                                                                   >  4:264290/1‑118 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                        <  8:26931/127‑1 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                        >  7:26931/1‑127 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCAc                                                                                  <  6:264259/149‑1 (MQ=255)
                                                            gCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCg                                                                                >  3:216860/1‑149 (MQ=255)
                                                                 gcgcATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                           >  3:47628/1‑149 (MQ=255)
                                                                      tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                    >  8:73936/1‑119 (MQ=255)
                                                                      tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                    <  7:73936/119‑1 (MQ=255)
                                                                                  gCCTGGCGGTGGCGCAACCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGca                                                            >  4:142577/1‑147 (MQ=255)
                                                                                       gCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCATCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaa                                                      >  8:45865/1‑148 (MQ=255)
                                                                                         ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                   <  3:144990/149‑1 (MQ=255)
                                                                                         ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                   <  7:45865/149‑1 (MQ=255)
                                                                                                cATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTGATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTgg                                            >  4:62608/1‑149 (MQ=255)
                                                                                                    cTCGTCGAAGTACAGCTCGCTGTAGCAGTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCaa                                         <  4:204914/148‑1 (MQ=255)
                                                                                                    cTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAAc                                        >  7:132935/1‑149 (MQ=255)
                                                                                                     tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                       >  8:190363/1‑149 (MQ=255)
                                                                                                     tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                       >  4:235066/1‑149 (MQ=255)
                                                                                                       gtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCt                                     >  1:240623/1‑149 (MQ=255)
                                                                                                          gtgctACAGCGAGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGAACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                   >  2:98310/5‑148 (MQ=255)
                                                                                                           aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                           >  3:236482/1‑139 (MQ=255)
                                                                                                           aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                           <  4:236482/139‑1 (MQ=255)
                                                                                                                cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                >  2:67142/1‑145 (MQ=255)
                                                                                                                cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                <  1:67142/145‑1 (MQ=255)
                                                                                                                  gCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCg                          <  5:71038/149‑1 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTTTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTCTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                     >  8:83314/1‑102 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                     <  7:83314/102‑1 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGc                        <  4:47628/147‑1 (MQ=255)
                                                                                                                            gCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                    <  2:32567/65‑1 (MQ=255)
                                                                                                                                   tcgGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGaa         <  4:216860/149‑1 (MQ=255)
                                                                                                                                       tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                           >  4:209897/4‑79 (MQ=38)
                                                                                                                                       tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                           <  3:209897/76‑1 (MQ=38)
                                                                                                                                          cgATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCggg  <  5:105315/149‑1 (MQ=255)
                                                                                                                                             tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                <  1:159347/116‑1 (MQ=255)
                                                                                                                                             tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                >  2:159347/1‑116 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GGTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGG  >  NC_002947/1780596‑1780882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑