Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,780,743 A→C 7.4% F201V (TTC→GTC)  dapC ← N‑succinyl‑L,L‑diaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,780,7430AC 7.4% 165.9 / 4.1 54F201V (TTC→GTC) dapCN‑succinyl‑L,L‑diaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base C (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACC  >  NC_002947/1780596‑1780890
                                                                                                                                                   |                                                                                                                                                   
ggTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGTAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAg                                                                                                                                                    >  6:71038/1‑149 (MQ=255)
ggTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAg                                                                                                                                                    <  1:18618/149‑1 (MQ=255)
        cgcTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTc                                                                                                                                            <  4:170824/149‑1 (MQ=255)
             ggACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCa                                                                                                                                        <  4:95448/148‑1 (MQ=255)
                   gCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAggg                                                                                                                                 >  4:253658/1‑149 (MQ=255)
                     tgtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGc                                                                                                                                >  4:144990/1‑148 (MQ=255)
                      gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                         <  8:281486/138‑3 (MQ=255)
                      gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                         >  7:281486/1‑136 (MQ=255)
                        ggAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCATTc                                                                                                                            >  6:105315/1‑149 (MQ=255)
                           acacCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGc                                                                                                                         <  7:242589/149‑1 (MQ=255)
                                 cGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGaa                                                                                                                                                     >  5:208414/1‑115 (MQ=255)
                                 cGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGaa                                                                                                                                                     <  6:208414/115‑1 (MQ=255)
                                         gcAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGAGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                            >  7:219701/1‑148 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                <  5:247765/131‑1 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                >  6:247765/1‑131 (MQ=255)
                                                      gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAgc                                                                                              <  6:326749/149‑1 (MQ=255)
                                                        ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCa                                                                                                                           <  3:264290/118‑1 (MQ=255)
                                                        ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGATCGTCAGCCAGGGCAATCa                                                                                                                           >  4:264290/1‑118 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                <  8:26931/127‑1 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                >  7:26931/1‑127 (MQ=255)
                                                          aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCAc                                                                                          <  6:264259/149‑1 (MQ=255)
                                                            gCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCg                                                                                        >  3:216860/1‑149 (MQ=255)
                                                                 gcgcATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                   >  3:47628/1‑149 (MQ=255)
                                                                      tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                            >  8:73936/1‑119 (MQ=255)
                                                                      tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                            <  7:73936/119‑1 (MQ=255)
                                                                                  gCCTGGCGGTGGCGCAACCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGca                                                                    >  4:142577/1‑147 (MQ=255)
                                                                                       gCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCATCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaa                                                              >  8:45865/1‑148 (MQ=255)
                                                                                         ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                           <  3:144990/149‑1 (MQ=255)
                                                                                         ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                           <  7:45865/149‑1 (MQ=255)
                                                                                                cATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTGATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTgg                                                    >  4:62608/1‑149 (MQ=255)
                                                                                                    cTCGTCGAAGTACAGCTCGCTGTAGCAGTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCaa                                                 <  4:204914/148‑1 (MQ=255)
                                                                                                    cTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAAc                                                >  7:132935/1‑149 (MQ=255)
                                                                                                     tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                               >  8:190363/1‑149 (MQ=255)
                                                                                                     tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                               >  4:235066/1‑149 (MQ=255)
                                                                                                       gtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCt                                             >  1:240623/1‑149 (MQ=255)
                                                                                                          gtgctACAGCGAGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGAACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                           >  2:98310/5‑148 (MQ=255)
                                                                                                           aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                                   <  4:236482/139‑1 (MQ=255)
                                                                                                           aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                                   >  3:236482/1‑139 (MQ=255)
                                                                                                                cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                        <  1:67142/145‑1 (MQ=255)
                                                                                                                cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                        >  2:67142/1‑145 (MQ=255)
                                                                                                                  gCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCg                                  <  5:71038/149‑1 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTTTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTCTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                             >  8:83314/1‑102 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                             <  7:83314/102‑1 (MQ=255)
                                                                                                                      gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGc                                <  4:47628/147‑1 (MQ=255)
                                                                                                                            gCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                            <  2:32567/65‑1 (MQ=255)
                                                                                                                                   tcgGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGaa                 <  4:216860/149‑1 (MQ=255)
                                                                                                                                       tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                   <  3:209897/76‑1 (MQ=38)
                                                                                                                                       tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                   >  4:209897/4‑79 (MQ=38)
                                                                                                                                          cgATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCggg          <  5:105315/149‑1 (MQ=255)
                                                                                                                                             tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                        >  2:159347/1‑116 (MQ=255)
                                                                                                                                             tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                        <  1:159347/116‑1 (MQ=255)
                                                                                                                                              cACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                           >  3:61534/1‑112 (MQ=255)
                                                                                                                                              cACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                           <  4:61534/112‑1 (MQ=255)
                                                                                                                                                 gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACg                         >  7:21121/1‑127 (MQ=255)
                                                                                                                                                 gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACg                         <  8:21121/127‑1 (MQ=255)
                                                                                                                                                 gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTAATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGATCACAGGAACAGGATCTGGCAACGCTTCCCCACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAAc   >  2:313877/1‑149 (MQ=255)
                                                                                                                                                  aaGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAAcc  <  8:132935/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTTGGAGCGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACC  >  NC_002947/1780596‑1780890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑