Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,780,750 T→A 8.7% E198D (GAA→GAT)  dapC ← N‑succinyl‑L,L‑diaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,780,7500TA 8.7% 152.5 / 3.9 54E198D (GAA→GAT) dapCN‑succinyl‑L,L‑diaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base A (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACCGTTGT  >  NC_002947/1780604‑1780895
                                                                                                                                                  |                                                                                                                                                 
cgcTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTc                                                                                                                                                 <  4:170824/149‑1 (MQ=255)
     ggACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCa                                                                                                                                             <  4:95448/148‑1 (MQ=255)
           gCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAggg                                                                                                                                      >  4:253658/1‑149 (MQ=255)
             tgtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGc                                                                                                                                     >  4:144990/1‑148 (MQ=255)
              gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                              <  8:281486/138‑3 (MQ=255)
              gtgGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGAACACGACTTCGTGATCgcc                                                                                                                                              >  7:281486/1‑136 (MQ=255)
                ggAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCATTc                                                                                                                                 >  6:105315/1‑149 (MQ=255)
                   acacCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGc                                                                                                                              <  7:242589/149‑1 (MQ=255)
                                 gcAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGAGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                                 >  7:219701/1‑148 (MQ=255)
                                              gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                     >  6:247765/1‑131 (MQ=255)
                                              gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                     <  5:247765/131‑1 (MQ=255)
                                              gACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAgc                                                                                                   <  6:326749/149‑1 (MQ=255)
                                                ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCa                                                                                                                                <  3:264290/118‑1 (MQ=255)
                                                ccAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGATCGTCAGCCAGGGCAATCa                                                                                                                                >  4:264290/1‑118 (MQ=255)
                                                  aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                     <  8:26931/127‑1 (MQ=255)
                                                  aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAgg                                                                                                                     >  7:26931/1‑127 (MQ=255)
                                                  aaGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCAc                                                                                               <  6:264259/149‑1 (MQ=255)
                                                    gCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCg                                                                                             >  3:216860/1‑149 (MQ=255)
                                                         gcgcATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                        >  3:47628/1‑149 (MQ=255)
                                                              tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                                 >  8:73936/1‑119 (MQ=255)
                                                              tGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                                 <  7:73936/119‑1 (MQ=255)
                                                                          gCCTGGCGGTGGCGCAACCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGca                                                                         >  4:142577/1‑147 (MQ=255)
                                                                               gCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCATCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaa                                                                   >  8:45865/1‑148 (MQ=255)
                                                                                 ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                                <  3:144990/149‑1 (MQ=255)
                                                                                 ggTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCacaggaacag                                                                <  7:45865/149‑1 (MQ=255)
                                                                                        cATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTGATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTgg                                                         >  4:62608/1‑149 (MQ=255)
                                                                                            cTCGTCGAAGTACAGCTCGCTGTAGCAGTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCaa                                                      <  4:204914/148‑1 (MQ=255)
                                                                                            cTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAAc                                                     >  7:132935/1‑149 (MQ=255)
                                                                                             tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                                    >  8:190363/1‑149 (MQ=255)
                                                                                             tcgtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACg                                                    >  4:235066/1‑149 (MQ=255)
                                                                                               gtcgAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCt                                                  >  1:240623/1‑149 (MQ=255)
                                                                                                  gtgctACAGCGAGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGAACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                                >  2:98310/5‑148 (MQ=255)
                                                                                                   aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                                        >  3:236482/1‑139 (MQ=255)
                                                                                                   aaGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTCCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGc                                                        <  4:236482/139‑1 (MQ=255)
                                                                                                        cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                             <  1:67142/145‑1 (MQ=255)
                                                                                                        cAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCCGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                             >  2:67142/1‑145 (MQ=255)
                                                                                                          gCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCg                                       <  5:71038/149‑1 (MQ=255)
                                                                                                              gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTTTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTCTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                                  >  8:83314/1‑102 (MQ=255)
                                                                                                              gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGcc                                                                                  <  7:83314/102‑1 (MQ=255)
                                                                                                              gCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGc                                     <  4:47628/147‑1 (MQ=255)
                                                                                                                    gCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGgtgt                                                                                                                 <  2:32567/65‑1 (MQ=255)
                                                                                                                           tcgGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGaa                      <  4:216860/149‑1 (MQ=255)
                                                                                                                               tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                        >  4:209897/4‑79 (MQ=38)
                                                                                                                               tgacgAACACGACTTCGTGATCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAgg                                                                                        <  3:209897/76‑1 (MQ=38)
                                                                                                                                  cgATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCggg               <  5:105315/149‑1 (MQ=255)
                                                                                                                                     tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                             <  1:159347/116‑1 (MQ=255)
                                                                                                                                     tCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCcac                                             >  2:159347/1‑116 (MQ=255)
                                                                                                                                      cACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                                >  3:61534/1‑112 (MQ=255)
                                                                                                                                      cACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTc                                                <  4:61534/112‑1 (MQ=255)
                                                                                                                                         gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACg                              <  8:21121/127‑1 (MQ=255)
                                                                                                                                         gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACg                              >  7:21121/1‑127 (MQ=255)
                                                                                                                                         gAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTAATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGATCACAGGAACAGGATCTGGCAACGCTTCCCCACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAAc        >  2:313877/1‑149 (MQ=255)
                                                                                                                                          aaGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAAcc       <  8:132935/149‑1 (MQ=255)
                                                                                                                                             tCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACCgtt    <  3:235066/149‑1 (MQ=255)
                                                                                                                                               gtgtTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACCgttgt  >  2:27592/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCTTGGACAGGCTGTGGAACACCACGCAGCGCGCAAAATCGCTACGACCAAGCTCGGCGCATGCCGACAGCAGGCCTGGCGGTGGCGCATCCTCGTCGAAGTACAGCTCGCTGTAGCACTCGTCGGCGGCGATCACGAAGTCGTGTTCGTCAGCCAGGGCAATCAGCTTCTTCAGGGTGTTCATCGGCACCAGCGCACCGGTAGGGTTGCCTGGCGAGCACAGGAACAGGATCTGGCAACGCTTCCACACCTCGGCCGGTACGGCGTCGAAGTCCGGGTTGAAACCGTTGT  >  NC_002947/1780604‑1780895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑