Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	220,623	C→G	6.5%	intergenic (+80/‑48)	PP_0168 → / → PP_5431	putative surface adhesion protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	220,623	0	C	G	6.5%	185.6 / 4.0	62	intergenic (+80/‑48)	PP_0168/PP_5431	putative surface adhesion protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base G (2/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

ACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATGCTCCC  >  NC_002947/220481‑220771                                                                                                                                               |                                                                                                                                                      aCGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACACGCACAGCTGACCCACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCg‑g                                                                                                                                                <  6:262502/149‑1 (MQ=255)  cGATCAACTCGTTTACCCGCGGCCCCGACCCGACCATCATCACCCCCAACACGCACCGCCGACACCCCCCCGGGGCCCCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCGCGCAGGCCCG‑Gc                                                                                                                                               <  7:214747/149‑1 (MQ=255)      cAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATc                                                                                                                                           >  3:202639/1‑149 (MQ=255)          tCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGGCGGCGCGGCCG‑GCgctggttt                                                                                                                                       >  1:149515/1‑142 (MQ=255)             ttGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCGTACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGcc                                                                                                                                     >  6:257735/1‑148 (MQ=255)                aTCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTAtg                                                                                                                                 >  5:165305/1‑149 (MQ=255)                 tCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTAtgc                                                                                                                                >  1:10150/1‑149 (MQ=255)                       gcgcCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCCGGCCTGCGCGCCCG‑GCGAACATTTCCAATGctgctg                                                                                                                           >  3:282049/1‑148 (MQ=255)                           cGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTAc                                                                                                                      <  6:165305/149‑1 (MQ=255)                             aTCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCGCGCAGGCCCG‑Gc                                                                                                                                               <  8:181601/122‑1 (MQ=255)                             aTCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCGCGCAGGCCCG‑Gc                                                                                                                                               >  7:181601/1‑122 (MQ=255)                                    catcatcatCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGa                                                                                                             <  4:202639/149‑1 (MQ=255)                                         tcatcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGAGCGAAATTTCCAAATCATCATGTCTACAAACAAGGaacc                                                                                                          >  1:105009/1‑146 (MQ=255)                                                   caaGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAgg                                                                                              <  6:93311/149‑1 (MQ=255)                                                      ggACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGa                                                                                            >  8:29711/1‑148 (MQ=255)                                                          aGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACcaacaa                                                                                                                >  7:57219/1‑124 (MQ=255)                                                                   accaccGGTGCCGCAGAGGGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCt                                                                              <  7:216561/149‑1 (MQ=255)                                                                   accaccGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGtt                                                                                >  6:161973/1‑147 (MQ=255)                                                                   accaccGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGtt                                                                                <  5:161973/147‑1 (MQ=255)                                                                   accaccGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTc                                                                               >  7:144240/1‑148 (MQ=255)                                                                       ccGGGGCCGCACTGGGGCCCGGGATCTTTCCTTCTACCCGCCAACAGCGCATACTGTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGt                                                                          <  4:282049/149‑1 (MQ=255)                                                                            gCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCGTACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTgcaac                                                                     <  5:257735/149‑1 (MQ=255)                                                                                           cGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGTGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGc                                                       >  6:250851/1‑148 (MQ=255)                                                                                               tctTTCCTGTTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGtt                                                  >  8:89429/1‑149 (MQ=255)                                                                                               tctTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGtt                                                  >  5:28962/1‑149 (MQ=255)                                                                                                 tttCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgc                                                >  5:224381/1‑149 (MQ=255)                                                                                                 tttCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGAGACGAGGGACATCGCCATGTTCTAACTGCAACGCAAACCCCCCCGCCAGTtgc                                                >  6:21325/1‑149 (MQ=255)                                                                                                  ttCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgct                                               >  7:277613/1‑149 (MQ=255)                                                                                                     cTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgctgc                                             <  7:29711/148‑1 (MQ=255)                                                                                                     cTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgctgc                                             <  6:224381/148‑1 (MQ=255)                                                                                                     cTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCg                                            >  4:276011/1‑149 (MQ=255)                                                                                                       gCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCggg                                          >  5:232569/1‑149 (MQ=255)                                                                                                       gCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCggg                                          >  1:275162/1‑149 (MQ=255)                                                                                                        cTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGTGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGt                                         <  5:250851/149‑1 (MQ=255)                                                                                                        cTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGt                                         <  3:276011/149‑1 (MQ=255)                                                                                                        cTACCCGCCAACACCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCgtgt                                         <  3:16186/149‑1 (MQ=255)                                                                                                           cccGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTgg                                       <  2:149515/148‑1 (MQ=255)                                                                                                            ccGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGaa                                     <  2:10150/149‑1 (MQ=255)                                                                                                              gCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAag                                    >  2:24243/1‑148 (MQ=255)                                                                                                                cAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAagcag                                 <  7:89429/149‑1 (MQ=255)                                                                                                                  aCAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCTGGTGGAAGCAGcc                               >  4:14211/1‑149 (MQ=255)                                                                                                                       gcATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGccgccg                                                         <  2:232020/118‑1 (MQ=255)                                                                                                                       gcATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGccgccg                                                         >  1:232020/1‑118 (MQ=255)                                                                                                                         aTACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGttt                         >  5:5333/1‑148 (MQ=255)                                                                                                                          tACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGa                       <  5:21325/149‑1 (MQ=255)                                                                                                                           aCTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATTTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGAt                      >  7:79524/1‑149 (MQ=255)                                                                                                                            ctctGGCGCCGGGCCTGCGCGCCCG‑GCGATTTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGAt                      <  8:79524/148‑1 (MQ=255)                                                                                                                            ctctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATc                     <  2:275162/149‑1 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCggg                                          >  6:204412/1‑127 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCggg                                          <  5:204412/127‑1 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGt                           <  2:11139/142‑1 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGt                           >  1:11139/1‑142 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGAt                      >  2:241111/1‑147 (MQ=255)                                                                                                                             tctGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGAt                      >  3:246947/1‑147 (MQ=255)                                                                                                                              agatcgcCGGGCGCGCAGGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg                   >  3:95715/5‑149 (MQ=255)                                                                                                                                 gcgcCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCTGGTGGAAGCAGCCGCGTTTGATCAg                   <  3:14211/146‑1 (MQ=255)                                                                                                                                 gcgcCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg                   <  8:144240/146‑1 (MQ=255)                                                                                                                                 gcgcCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTCCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg                   <  1:241111/146‑1 (MQ=255)                                                                                                                                 gcgcCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCCGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGtt                 >  6:129414/1‑148 (MQ=255)                                                                                                                                    ccGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCAcc             <  6:232569/149‑1 (MQ=255)                                                                                                                                           tGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAAcgccgc                                                           >  7:11432/1‑96 (MQ=255)                                                                                                                                           tGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAAcgccgc                                                           <  8:11432/96‑1 (MQ=255)                                                                                                                                               cgcgcg‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTTACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATGCTccc  <  8:194791/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                      ACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCG‑GCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATGCTCCC  >  NC_002947/220481‑220771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑