| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,788,630 | G→C | 8.6% | V35L (GTG→CTG) ‡ | uppS → | (2E,6E)‑farnesyl‑diphosphate specific ditrans,polycis‑undecaprenyl‑diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,788,630 | 0 | G | C | 8.6% | 171.7 / 7.6 | 58 | V35L (GTG→CTG) ‡ | uppS | (2E,6E)‑farnesyl‑diphosphate specific ditrans,polycis‑undecaprenyl‑diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (24/29); new base C (3/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
AAGGAAAAGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGATGGAGC > NC_002947/1788486‑1788774 | aaGGAAAAGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGc > 7:283321/1‑149 (MQ=255) ggAAAAGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCg < 3:88673/149‑1 (MQ=255) aaaGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGc < 1:118979/149‑1 (MQ=255) aaGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGc > 7:194618/1‑148 (MQ=255) gCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGgtgt < 7:243102/149‑1 (MQ=255) gCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGgtgt < 3:81780/149‑1 (MQ=255) cGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTgg < 8:283321/149‑1 (MQ=255) cGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTgg < 8:194618/149‑1 (MQ=255) cTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATg > 5:63645/1‑149 (MQ=255) tAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGc > 6:72668/1‑149 (MQ=255) tAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGc > 4:218239/1‑149 (MQ=255) aGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATg < 4:304224/146‑1 (MQ=255) ggCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGt < 4:112926/149‑1 (MQ=255) ccGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTc < 8:136685/149‑1 (MQ=255) tAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCAt < 8:31539/148‑1 (MQ=255) ggAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAg > 6:12885/1‑149 (MQ=255) cAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGcc > 8:245019/1‑148 (MQ=255) cAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTcc > 7:112878/1‑149 (MQ=255) ggcgCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCgggg > 8:278281/1‑149 (MQ=255) gcgcCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCg < 2:1594/127‑1 (MQ=255) gcgcCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCg > 1:1594/1‑127 (MQ=255) tCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCCGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCtgtg > 5:140882/1‑127 (MQ=255) tCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCCGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCtgtg < 6:140882/127‑1 (MQ=255) ggTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCg > 8:113521/1‑134 (MQ=255) ggTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCg < 7:113521/134‑1 (MQ=255) gTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTc < 8:112878/149‑1 (MQ=255) aTGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGcc > 8:285815/1‑149 (MQ=255) gcgATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTtctc < 7:245019/149‑1 (MQ=255) gATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTtctc > 2:11185/1‑147 (MQ=255) tcatcaTGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGc < 2:172204/149‑1 (MQ=255) catcatGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGc > 1:189755/1‑148 (MQ=255) tcatGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCggg > 5:195356/1‑113 (MQ=255) tcatGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCggg < 6:195356/113‑1 (MQ=255) catGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCgg > 1:100207/1‑65 (MQ=255) catGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCgg < 2:100207/65‑1 (MQ=255) atggatggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCtgtg < 7:281013/100‑1 (MQ=255) atggatggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCtgtg > 8:281013/1‑100 (MQ=255) atggatggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACt > 2:282778/1‑122 (MQ=255) atggatggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACt < 1:282778/122‑1 (MQ=255) tggatggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAAc < 6:100461/149‑1 (MQ=255) atggCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTgg > 5:131909/1‑149 (MQ=255) aacaacCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTc < 6:225209/123‑1 (MQ=255) aacaacCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAgcg > 4:176274/1‑149 (MQ=255) ccGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTg < 5:72668/149‑1 (MQ=255) ccGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTg < 3:218239/149‑1 (MQ=255) ggCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGTCGaa < 3:176274/148‑1 (MQ=255) cGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAagag < 5:12885/149‑1 (MQ=255) gAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGCCCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAgg > 7:275239/1‑149 (MQ=255) aaCGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGt < 7:278281/149‑1 (MQ=255) ccGCCTTGTGCCCGGCCACGCCGGGCAGCAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAaga < 3:122858/140‑1 (MQ=38) ccGCCTTGTGCCCGGCCACGCCGGGCAGCAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAaga > 4:122858/2‑141 (MQ=38) ccttgtgcCCGGCCACGCCGGGCAGCAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTgcgc > 1:238706/6‑149 (MQ=255) cttgtgcCCGGCCACGCCGGGCAGCAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCt > 2:210218/5‑149 (MQ=255) tgctgcCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCt > 8:42339/1‑148 (MQ=255) ctgcCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGAt < 2:189755/149‑1 (MQ=255) tgcCCGGCCACGCCGGGCAGCAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGAt < 2:238706/148‑1 (MQ=255) ccGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGATGGAg < 8:38303/149‑1 (MQ=255) cGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGATGGAGc > 4:39526/1‑149 (MQ=255) | AAGGAAAAGGACCTGATGGCCGTCTAAGGCCGGGGTTTTTTAATGGAAAAGACCAAGCCAGCGGCGCCGTCCTCGGTGCCGCGTCATGTCGCGATCATCATGGATGGCAACAACCGCTGGGCGAAAAAACGCCTGCTGCCCGGCGTGGCCGGGCACAAGGCGGGTGTGGATGCCGTTCGCGCGGTCATCGAAGTCTGTGCCAAGTCCGGGGTCGAGGTACTGACCCTGTTCGCCTTCTCCAGCGAGAACTGGCAGCGCCCTGCCGAAGAGGTCGGTGCGCTGATGGAGC > NC_002947/1788486‑1788774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |