Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,797,971 C→G 7.5% T224T (ACC→ACG)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,797,9710CG 7.5% 153.3 / 2.2 51T224T (ACC→ACG) lpxAacyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (25/22);  new base G (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACGTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGTGAGGCCAGT  >  NC_002947/1797824‑1798119
                                                                                                                                                   |                                                                                                                                                    
ggACGTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCg                                                                                                                                                     >  5:310947/1‑149 (MQ=255)
 gACGTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACGGTCAGGCCCTGACGGt                                                                                                                                                    <  8:295492/149‑1 (MQ=255)
  aCGTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTa                                                                                                                                                   <  7:178058/149‑1 (MQ=255)
    gTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTag                                                                                                                                                  >  5:165805/1‑148 (MQ=255)
       ccGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTagaaga                                                                                                                                              >  8:233598/1‑149 (MQ=255)
             ttCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCt                                                                                                                                        >  8:71119/1‑149 (MQ=255)
             ttCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCt                                                                                                                                        >  8:66826/1‑149 (MQ=255)
                    ccGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAg                                                                                                                                 >  4:77226/1‑149 (MQ=255)
                    ccGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATCGTCTACCGTCAGGGCCTGACCGTag                                                                                                                                                  <  1:5126/132‑1 (MQ=255)
                    ccGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATCGTCTACCGTCAGGGCCTGACCGTag                                                                                                                                                  >  2:5126/1‑132 (MQ=255)
                      gtgtTCGGCAGCCCGGCCGAAGCCCGCTGCATGAACTTCGAGGGCTTGCGCCGCGGGGGTGTGAGCGACGTGGTGATCCAAGTGCTTCGTCGTTGCTACAAGATTGTCTACCGTCAGGGGCTGAGGGTAGAAGACGCGCTCAAGGAGCt                                                                                                                               <  3:295204/149‑1 (MQ=255)
                      gtgtTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCt                                                                                                                               <  2:284078/149‑1 (MQ=255)
                            ggCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTa                                                                                                                                                   >  3:42779/1‑123 (MQ=255)
                            ggCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTa                                                                                                                                                   <  4:42779/123‑1 (MQ=255)
                            ggCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCg                                                                                                                          >  2:291848/1‑148 (MQ=255)
                               aGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAAcc                                                                                                                      <  1:291848/149‑1 (MQ=255)
                                   ccgccgAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTa                                                                                                                                                   >  4:281681/1‑116 (MQ=255)
                                   ccgccgAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTa                                                                                                                                                   <  3:281681/116‑1 (MQ=255)
                                   ccgccgAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGATGCTACAAGATTGTCTAACGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGcc                                                                                                                  >  6:195495/1‑149 (MQ=255)
                                                 agcaTGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGa                                                                                                    <  7:233598/149‑1 (MQ=255)
                                                       aaCTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGa                                                                                              <  3:57695/149‑1 (MQ=255)
                                                               gggCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTcc                                                                                      >  6:278974/1‑149 (MQ=255)
                                                                               ggTGTCAGCGACGAGGTGATCCATGTGCTGCGTCGGTGCTACAAGATTGTCTCCCGGCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGACCCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAg                                                                      <  4:33883/149‑1 (MQ=255)
                                                                                   tCAGCGACGAGGTGATCCATGTGCTGAGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTcc                                                                  >  2:111776/1‑149 (MQ=255)
                                                                                         acgaGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTcgc                                                            <  5:278974/149‑1 (MQ=255)
                                                                                                  tCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCAcg                                                   <  3:77226/149‑1 (MQ=255)
                                                                                                     aTGTGCTGCGTCGTTGCTACAGGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACgcgc                                                >  7:108722/1‑149 (MQ=255)
                                                                                                     aTGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAg                                                                      >  5:180724/1‑127 (MQ=255)
                                                                                                     aTGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAg                                                                      <  6:180724/127‑1 (MQ=255)
                                                                                                     aTGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACgcgc                                                <  7:71119/149‑1 (MQ=255)
                                                                                                     aTGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACgcgc                                                <  7:66826/149‑1 (MQ=255)
                                                                                                                     cTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCt                                               <  6:300476/134‑1 (MQ=255)
                                                                                                                     cTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCt                                               >  5:300476/1‑134 (MQ=255)
                                                                                                                      tACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCttt                               >  2:272161/1‑149 (MQ=255)
                                                                                                                        cAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCt                                                               <  5:212424/115‑1 (MQ=255)
                                                                                                                        cAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCt                                                               >  6:212424/1‑115 (MQ=255)
                                                                                                                         aaGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCg                            <  1:272161/149‑1 (MQ=255)
                                                                                                                           cgtcttCTACGGTCAGGCCCTGACGGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCg                            >  8:81933/6‑147 (MQ=255)
                                                                                                                            gtcttCTACGGTCAGGCCCTGACGGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAg                         >  6:55838/5‑149 (MQ=255)
                                                                                                                                tCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCt                     >  3:289367/1‑149 (MQ=255)
                                                                                                                                   aCCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGt                  >  7:31708/1‑149 (MQ=255)
                                                                                                                                      gTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGc               >  6:81431/1‑149 (MQ=255)
                                                                                                                                          gggCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGt           <  5:195495/149‑1 (MQ=255)
                                                                                                                                           ggCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCg                >  2:232345/1‑143 (MQ=255)
                                                                                                                                           ggCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCg                <  1:232345/143‑1 (MQ=255)
                                                                                                                                           ggCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGACCACCTTTGc                             >  2:262811/1‑130 (MQ=255)
                                                                                                                                           ggCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTAGATCCTGATCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGc                             <  1:262811/130‑1 (MQ=255)
                                                                                                                                             ccTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGTGAg        <  8:108722/149‑1 (MQ=255)
                                                                                                                                              cTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGTGAgg       >  5:186438/1‑149 (MQ=255)
                                                                                                                                                gACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGt           >  2:312200/1‑143 (MQ=255)
                                                                                                                                                gACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGt           <  1:312200/143‑1 (MQ=255)
                                                                                                                                                   cGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGTGAGGCCAGt  >  7:243859/1‑149 (MQ=255)
                                                                                                                                                   cGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGACATGGCCCAGCTTTGCGTAGCGCTGGTCGCCg             >  2:166248/1‑138 (MQ=255)
                                                                                                                                                   cGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGACATGGCCCAGCTTTGCGTAGCGCTGGTCGCCg             <  1:166248/138‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGACGTTCCGGCCTTCGTTACCGTGTTCGGCAGCCCCGCCGAAGCCCGCAGCATGAACTTCGAGGGCATGCGCCGCCGCGGTTTCAGCGACGAGGTGATCCATGTGCTGCGTCGTTGCTACAAGATTGTCTACCGTCAGGGCCTGACCGTAGAAGACGCGCTCAAGGAGCTGGCCGAACCGGCCACGCAACACCCTGAGGTCGAGCTGTTCCGTCAGTCGATCCTGAGCTCCGCTCGCGGCATCACGCGCTGATATGGCCCAGCTTTGCGTAGCGCTGGTCGCCGGTGAGGCCAGT  >  NC_002947/1797824‑1798119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑