Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,802,443 G→C 9.0% M809I (ATG→ATC) ‡ dnaEA → DNA polymerase III subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,802,4430GC 9.0% 128.3 / 2.7 43M809I (ATG→ATC) ‡dnaEADNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base C (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CCACTCCGCCGCCTATGGCCTGGTGTCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGAGGCTC  >  NC_002947/1802299‑1802579
                                                                                                                                                |                                                                                                                                        
ccACTCCGCCGCCTATGGCCTGGTGTCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAg                                                                                                                                       <  7:243799/148‑1 (MQ=255)
       gccgccTATGGCCTGGTGTCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCTTCATGCTgcgcac                                                                                                                               <  3:5096/149‑3 (MQ=255)
             tATGGCCTGGTGTCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGAcgc                                                                                                                         >  4:82414/1‑149 (MQ=255)
                        gtCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATACATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTg                                                                                                               >  3:160924/1‑148 (MQ=255)
                               cAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACgcgc                                                                                                                       >  1:196908/1‑133 (MQ=255)
                               cAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACgcgc                                                                                                                       <  2:196908/133‑1 (MQ=255)
                               cAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTtctc                                                                                                       <  3:82414/149‑1 (MQ=255)
                                                  cccACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGt                                                                                     >  6:182749/1‑148 (MQ=255)
                                                          ccGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAacga                                                                            >  1:122444/1‑149 (MQ=255)
                                                              cgcCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGc                                                                        >  1:226405/1‑149 (MQ=255)
                                                               gcCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGcc                                                                       <  6:196264/149‑1 (MQ=255)
                                                               gcCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGcc                                                                       <  4:160924/149‑1 (MQ=255)
                                                                  aTTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTa                                                                    >  3:250969/1‑149 (MQ=255)
                                                                  aTTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTa                                                                    >  3:83631/1‑149 (MQ=255)
                                                                     cATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATc                                                                  <  4:250969/148‑1 (MQ=255)
                                                                      aTGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGt                                                                >  1:10655/1‑149 (MQ=255)
                                                                          ccGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTAc                                                            >  3:277473/1‑149 (MQ=255)
                                                                                tCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCtt                                                      >  5:171714/1‑149 (MQ=255)
                                                                                     tCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGt                                                   <  6:171714/147‑1 (MQ=255)
                                                                                     tCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTg                                                  <  4:277473/148‑1 (MQ=255)
                                                                                             tatGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAgg                                         <  2:226405/149‑1 (MQ=255)
                                                                                                gCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGGCGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGgtg                                      >  5:217689/1‑149 (MQ=255)
                                                                                                gCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGgtg                                      >  8:79913/1‑149 (MQ=255)
                                                                                                 cATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAgg                                         >  7:30566/1‑145 (MQ=255)
                                                                                                 cATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAgg                                         <  8:30566/145‑1 (MQ=255)
                                                                                                  aTAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACgg                                                                         <  5:287681/112‑1 (MQ=255)
                                                                                                  aTAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACgg                                                                         >  6:287681/1‑112 (MQ=255)
                                                                                                               ggtggtggtGCTGGTGGAGGAGGTGCGCAGCATGAAGCTgc                                                                                                                                   >  1:12745/1‑41 (MQ=255)
                                                                                                               ggtggtggtGCTGGTGGAGGAGGTGCGCAGCATGAAGCTgc                                                                                                                                   <  2:12745/41‑1 (MQ=255)
                                                                                                                gtggtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGc                                 <  2:314669/138‑1 (MQ=255)
                                                                                                                gtggtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGc                                 >  1:314669/1‑138 (MQ=255)
                                                                                                                gtggtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGt                      >  7:97351/1‑149 (MQ=255)
                                                                                                                   gtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAg                                          <  7:274837/126‑1 (MQ=255)
                                                                                                                   gtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAg                                          >  8:274837/1‑126 (MQ=255)
                                                                                                                   gtggtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGa                   <  2:10655/149‑1 (MQ=255)
                                                                                                                      gtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGgtg                                      <  7:247291/127‑1 (MQ=255)
                                                                                                                      gtgCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGgtg                                      >  8:247291/1‑127 (MQ=255)
                                                                                                                                aggaggTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGAg      <  4:83631/149‑1 (MQ=255)
                                                                                                                                    gaggcgcAGCTTCATGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGAGGCTc  >  4:269380/4‑149 (MQ=255)
                                                                                                                                     aggcgcAGCTTCATGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGa       >  7:55405/3‑143 (MQ=255)
                                                                                                                                     aggcgcAGCTTCATGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGa       <  8:55405/141‑1 (MQ=255)
                                                                                                                                             caTGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCg        >  7:86862/1‑134 (MQ=255)
                                                                                                                                             caTGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCg        <  8:86862/134‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
CCACTCCGCCGCCTATGGCCTGGTGTCGTACCAGACCGCCTGGCTGAAAACCCACTACCCGGCGCCATTCATGGCCGCGGTCCTGTCGGCGGATATGCATAACACCGACAAGGTGGTGGTGCTGGTGGAGGAGGTGCGCAGCATGAAGCTGCGCCTCGACGCGCCGGACGTGAACTTCTCGGACTTCAAGTTCACCGTCAACAACGACGGCCGTATCGTCTACGGCCTTGGTGCCATCAAGGGTGTCGGCGAAGGCCCGGTGGAGGCGATTGTCGAGGCTC  >  NC_002947/1802299‑1802579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑