Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,810,336 C→A 10.0% intergenic (+97/‑72) eno → / → ftsB enolase/cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,810,3360CA10.0% 123.9 / 6.9 50intergenic (+97/‑72)eno/ftsBenolase/cell division protein
Reads supporting (aligned to +/- strand):  ref base C (27/18);  new base A (3/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

ACTGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGGT  >  NC_002947/1810191‑1810482
                                                                                                                                                 |                                                                                                                                                  
aCTGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAACACAGGCCGCGGc                                                                                                                                                 >  2:143285/1‑149 (MQ=255)
    ggCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCa                                                                                                                                               <  5:135600/147‑1 (MQ=255)
     gcgcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGc                                                                                                                                            <  7:91422/149‑1 (MQ=255)
         cAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCg                                                                                                                                        >  3:226127/1‑149 (MQ=255)
                                ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                                <  5:107170/134‑1 (MQ=255)
                                ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                                >  6:107170/1‑134 (MQ=255)
                                 cGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTaa                                                                                                                 >  2:49899/1‑148 (MQ=255)
                                 cGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAc                                                                                                                <  3:224343/149‑1 (MQ=255)
                                      ttCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCAGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCACCGAAGTAACAAgaa                                                                                                           >  7:30993/1‑147 (MQ=255)
                                                aGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCgg                                                                                                      >  3:278118/1‑144 (MQ=255)
                                                aGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCgg                                                                                                      <  4:278118/144‑1 (MQ=255)
                                                   aaGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCttt                                                                                              <  4:226127/149‑1 (MQ=255)
                                                       gaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTaa                                                                                          <  4:148557/149‑1 (MQ=255)
                                                        aTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGACAGCCTTTTTAAc                                                                                         >  1:250351/1‑149 (MQ=255)
                                                          ggTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAg                                                                                                   <  7:245059/137‑1 (MQ=255)
                                                          ggTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTAGAGGCCCCGAAGTAACAAGTACGCGGCAg                                                                                                   >  8:245059/1‑137 (MQ=255)
                                                             aaaaaGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGCAGCCTGGCCTCGGCCAGGCGTCGTGCTAGTAGAGGCCCCGAAGGAACAAGTACGCGGCCGCCGTTTTagatggg                                                                                     >  8:173130/1‑142 (MQ=255)
                                                             aaaaaGGCAGCAGCCGGAGTTGTAGGAACGTGCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtttt                                                                                             <  7:173130/140‑1 (MQ=255)
                                                                            ggAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGTCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAg                                                                     <  4:119220/149‑1 (MQ=255)
                                                                                      gAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGACAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTgg                                                           <  2:250351/149‑1 (MQ=255)
                                                                                        aCGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgtt                                                         <  1:300767/149‑1 (MQ=255)
                                                                                          gTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgt                                                       >  5:152449/1‑149 (MQ=255)
                                                                                          gTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTAAGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgt                                                       >  3:295693/1‑149 (MQ=255)
                                                                                                aCTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGTCTTCGTGCTATTAGAGGCACCGAAGTAACAATTACGCGGCAGCCTTTTTAACCTGGATAACATCATGCGCAGTACCTATTGGTTGTTCCTct                                                 >  3:39186/1‑148 (MQ=255)
                                                                                                 cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTc                                                  <  8:182403/147‑1 (MQ=255)
                                                                                                 cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTc                                                  >  7:182403/1‑147 (MQ=255)
                                                                                                  tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCAGGCCTCGGCCACGCGTCGTGCTATTAGAGGGCCCGAAGTAAAAAATAACCGGGACCCATTTTTAACTGGAATAAGTGTTGCGCAGAACCGATTTGTTTTTGCTCGtt                                               >  8:23284/1‑149 (MQ=255)
                                                                                                    aaCTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCt                                             <  1:49899/149‑1 (MQ=255)
                                                                                                            tACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTAGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGCTAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGGTCTGCTCTTGc                                     >  2:251439/1‑149 (MQ=255)
                                                                                                                   tAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                <  5:225668/147‑1 (MQ=255)
                                                                                                                   tAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                >  6:225668/1‑147 (MQ=255)
                                                                                                                     aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                                 <  8:139200/144‑1 (MQ=255)
                                                                                                                     aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                                 >  7:139200/1‑144 (MQ=255)
                                                                                                                     aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACCGGAAGCGCAGTCCCAATTGGTTGTTCCTCGTTATGCTATTGGTGTTGGGTg                            >  4:254669/1‑149 (MQ=255)
                                                                                                                     aCGGGTTTCTGTCGACGAAGCCATGCCTCGGCCAGGCTTCGTGCTATTAGAGGCCCCGAAGTAACAAATACGCGGCAGCCTTTTTAACCTGGAAAACGTGAAGAGCACTCCCTATTGGTTGTTACTCATTCTGCTCTTGCAGTTGGGTg                            >  8:32081/1‑149 (MQ=255)
                                                                                                                       gggTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTAGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGc                          >  4:44007/1‑149 (MQ=255)
                                                                                                                              tGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGGTATTCGACGCCCCGAAGTAACAACTACGCGGCCGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAg                    >  7:154722/1‑148 (MQ=255)
                                                                                                                                tCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAg                    <  7:23284/146‑1 (MQ=255)
                                                                                                                                tCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGt                   <  7:32081/147‑1 (MQ=255)
                                                                                                                                    cgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGcc             >  4:309655/1‑149 (MQ=255)
                                                                                                                                    cgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGcc             >  7:217674/1‑149 (MQ=255)
                                                                                                                                     gaAGCCTGTCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAAAGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCt            >  7:204316/1‑149 (MQ=255)
                                                                                                                                     gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCt            >  1:142008/1‑149 (MQ=255)
                                                                                                                                     gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGAAGTCACTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCt            >  4:257934/1‑149 (MQ=255)
                                                                                                                                          cTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgg        <  6:234525/148‑1 (MQ=255)
                                                                                                                                          cTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgg        >  5:234525/1‑148 (MQ=255)
                                                                                                                                              ccTCGGCCATGCTTCGTGCTATTCGAGGCCCCCTAGTAACAAGTACGCGGCAGCCGTTTTATCCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGGTCTTGCTGTTGTGTGGCCTGCAGTACCGCCTGtgggtggg   <  8:30993/149‑1 (MQ=255)
                                                                                                                                               cTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGtgggtggg   >  3:61885/1‑148 (MQ=255)
                                                                                                                                               cTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGtgggtggg   >  1:312106/1‑148 (MQ=255)
                                                                                                                                                tCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTgggtgggt  >  5:16141/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACTGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGGT  >  NC_002947/1810191‑1810482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑