Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,864,750 T→C 8.1% A28A (GCT→GCC)  hda → DnaA regulatory inactivator Hda

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,864,7500TC 8.1% 137.2 / 2.2 49A28A (GCT→GCC) hdaDnaA regulatory inactivator Hda
Reads supporting (aligned to +/- strand):  ref base T (20/25);  new base C (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGAG  >  NC_002947/1864603‑1864891
                                                                                                                                                   |                                                                                                                                             
cGGGTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAAtgct                                                                                                                                               <  7:244470/148‑1 (MQ=255)
   gTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCgg                                                                                                                                           >  8:276066/1‑149 (MQ=255)
       aGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCAt                                                                                                                                        <  1:274804/148‑1 (MQ=255)
        gACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCAtt                                                                                                                                       <  6:128664/148‑1 (MQ=255)
        gACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACAATCCGGGCGCCAATGCTGCGGCAtt                                                                                                                                       <  4:47809/148‑1 (MQ=255)
         aCTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGCCCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTgg                                                                                                                                     <  7:269530/149‑1 (MQ=255)
          cTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggc                                                                                                                                    <  6:55624/149‑2 (MQ=255)
             tGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGtt                                                                                                                                  <  7:276066/148‑1 (MQ=255)
              gCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggcgcc                                                                                                                                 <  2:181023/148‑5 (MQ=255)
                aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGATACGt                                                                                                                              >  8:94877/1‑149 (MQ=255)
                 gATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                             >  8:70869/1‑149 (MQ=255)
                  aTTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCGGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                             >  7:145514/1‑148 (MQ=255)
                         aCCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGc                                                                                                                      >  6:183895/1‑148 (MQ=255)
                         aCCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCt                                                                                                                     >  5:120862/1‑149 (MQ=255)
                           cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGtt                                                                                                                                  >  4:177483/1‑134 (MQ=255)
                           cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGtt                                                                                                                                  <  3:177483/134‑1 (MQ=255)
                           cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCGGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTAt                                                                                                                   <  8:145514/149‑1 (MQ=255)
                                  ccccAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGc                                                                                                            >  7:136970/1‑149 (MQ=255)
                                    ccAGCGGTCCCGTCGACCGCCCGCGAGCATGCAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCAACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCg                                                                                                          >  7:200167/1‑149 (MQ=255)
                                    ccAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCg                                                                                                          >  7:251679/1‑149 (MQ=255)
                                      aGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGAc                                                                                                        <  8:50373/149‑1 (MQ=255)
                                      aGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAAAGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGAc                                                                                                        >  4:281319/1‑149 (MQ=255)
                                       gCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGATACGTCGAGCGGCTATGCGAAGCCGACg                                                                                                       <  7:94877/149‑1 (MQ=255)
                                             ccGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCt                                                                                                 >  3:187033/1‑149 (MQ=255)
                                                 cGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGAc                                                                                             <  8:251679/149‑1 (MQ=255)
                                                      gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCgaga                                                                                        <  7:281100/149‑1 (MQ=255)
                                                      gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCgaga                                                                                        <  4:15126/149‑1 (MQ=255)
                                                      gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCgaga                                                                                        <  8:136970/149‑1 (MQ=255)
                                                      gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCga                                                                                          >  8:281100/1‑147 (MQ=255)
                                                         cgcgAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGTCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGcc                                                                                     <  3:281319/149‑1 (MQ=255)
                                                          gcgAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCt                                                                                    >  7:216394/1‑149 (MQ=255)
                                                           cgAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                             <  3:261635/107‑1 (MQ=255)
                                                           cgAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                             >  4:261635/1‑107 (MQ=255)
                                                                      ccaccGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCtgtg                                                                        <  7:70869/149‑1 (MQ=255)
                                                                       caccGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTgg                                                                       <  5:183895/149‑1 (MQ=255)
                                                                        accGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTggg                                                                      >  4:277514/1‑149 (MQ=255)
                                                                                 gTTGCGCCTGGGTGGGCGCCGGCGCGATGACGCCACCTGGATCCACTACTATCCGGGCGCCAATGCTGGGGCTTTGGGTTACGGCGTGCGGCCATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGGCGGGCAAGCAgg                                                             <  6:296448/149‑1 (MQ=255)
                                                                                 gTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAgg                                                             <  3:277514/149‑1 (MQ=255)
                                                                                 gTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAgg                                                             <  1:265048/149‑1 (MQ=255)
                                                                                  ttGGCCCTGGGGGAGCGTCGTCGCTATTACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCTGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCTAGAGCCTTATCTACCTGTGGGGCAAGCAgg                                                             <  8:200167/148‑1 (MQ=255)
                                                                                               tgCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTc                                                <  8:216394/148‑1 (MQ=255)
                                                                                                                  cACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCAc                                            >  3:305959/1‑133 (MQ=255)
                                                                                                                  cACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCAc                                            <  4:305959/133‑1 (MQ=255)
                                                                                                                           caACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCt                   >  4:220357/1‑149 (MQ=255)
                                                                                                                                 ctaTCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAg              <  4:187033/148‑1 (MQ=255)
                                                                                                                                  taTCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGc             >  4:304985/1‑148 (MQ=255)
                                                                                                                                    tCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAAc          >  1:87895/1‑149 (MQ=255)
                                                                                                                                        ggCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTgg      <  6:120862/149‑1 (MQ=255)
                                                                                                                                           cccAATGCCGCAGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGc     >  5:94129/2‑147 (MQ=255)
                                                                                                                                            ccAATGCCGCAGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGAg  >  7:219842/1‑149 (MQ=255)
                                                                                                                                                   tgcGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGc     >  6:60267/1‑139 (MQ=255)
                                                                                                                                                   tgcGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGc     <  5:60267/139‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGGGTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGAG  >  NC_002947/1864603‑1864891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑