Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,879,890 G→A 12.8% intergenic (+74/+62) PP_1686 → / ← PP_1687 glutathione peroxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,879,8900GA12.8% 96.5 / 5.9 39intergenic (+74/+62)PP_1686/PP_1687glutathione peroxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/18);  new base A (2/3);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

TCCAGCCTGACCAAACCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGCACACACACAAATGCCACCGGCATCGCCA  >  NC_002947/1879745‑1880037
                                                                                                                                                 |                                                                                                                                                   
tcCAGGATGACCAACCGGGTTGATCCCGGGTTCGGCGCTGCTATCTAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGGGGGGTTGCTTTAAAGCGCGAGGCAGTTTAGGCGACGCAGATTGCGACTTCAGGAGGCCg                                                                                                                                                  <  1:189358/149‑1 (MQ=37)
      cTGACCAAACCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTcc                                                                                                                                            <  6:158876/149‑1 (MQ=255)
         aCCAAACCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTg                                                                                                                                          >  8:108372/1‑148 (MQ=255)
          ccAAACCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTg                                                                                                                                          >  7:6346/1‑147 (MQ=255)
            aaaCCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCAACTTCAGGAGAACGGCCTCCTGAAGt                                                                                                                                      <  5:138382/149‑1 (MQ=39)
             aaCCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCAACTTCAGGAGAACGGCCTCCTGAAGtc                                                                                                                                     <  3:113567/149‑2 (MQ=39)
               ccGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGc                                                                                                                                   <  7:108372/149‑1 (MQ=255)
                 ggATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCAGTTCTCCTGAAGTTGCgg                                                                                                                                 <  5:325530/149‑1 (MQ=255)
                         ccGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatc                                                                                                                             >  1:313061/1‑145 (MQ=255)
                          cgcgTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatcaagat                                                                                                                        <  2:249840/149‑1 (MQ=255)
                          cgcgTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatc                                                                                                                             >  2:88902/1‑144 (MQ=255)
                           gcgTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatcaaga                                                                                                                         <  5:148125/147‑1 (MQ=255)
                           gcgTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatcaaga                                                                                                                         >  6:148125/1‑147 (MQ=255)
                             gTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGgatcaagatcaa                                                                                                                     <  4:150037/149‑1 (MQ=255)
                                         tATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGtg                                                                                                         >  2:11481/1‑149 (MQ=255)
                                              aaaaGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGtggctgg                                                                                                    >  2:59120/1‑149 (MQ=255)
                                              aaaaGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGtggctgg                                                                                                    >  7:260323/1‑149 (MQ=255)
                                              aaaaGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGtggctgg                                                                                                    <  1:59120/149‑1 (MQ=255)
                                                           cTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACtt                                                                                       >  4:45219/1‑149 (MQ=255)
                                                           cTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACt                                                                                        >  4:103057/1‑148 (MQ=255)
                                                              acaGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCa                                                                                     <  1:284091/148‑1 (MQ=255)
                                                                       aCCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGtgcatg                                                                            <  8:260323/148‑1 (MQ=255)
                                                                           ggCTGCGCTTGCTGAGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACgg                                                                       <  3:103057/149‑1 (MQ=255)
                                                                                cgcTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGtgaa                                                                   <  2:313061/148‑4 (MQ=255)
                                                                                cgcTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTaa                                                                    <  3:164430/147‑1 (MQ=255)
                                                                                       tgcGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGccacc                                                            >  7:5865/1‑148 (MQ=255)
                                                                                         cggcgTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAAc                                                         <  1:11481/149‑1 (MQ=255)
                                                                                             gTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGca                                                     <  3:45219/149‑1 (MQ=255)
                                                                                              ttGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGcac                                                    <  8:6346/149‑1 (MQ=255)
                                                                                                 cTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGcaccac                                                 >  7:38888/1‑149 (MQ=255)
                                                                                                 cTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGcaccac                                                 >  7:84965/1‑149 (MQ=255)
                                                                                                 cTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGcaccac                                                 >  8:183300/1‑149 (MQ=255)
                                                                                                     aaaGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGcaccaccac                                              <  1:88902/148‑1 (MQ=255)
                                                                                                          gcgAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCAccggccg                                        <  7:183300/149‑1 (MQ=255)
                                                                                                                         cGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGcacaca                         >  8:218703/1‑149 (MQ=255)
                                                                                                                                 cccgcaaCTTCAAGAGAACGGCCTCCTGAAGTCGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGCACACACACAAATg                 >  3:81085/7‑149 (MQ=255)
                                                                                                                                    gcaaCTTCAGGAGAACGGCCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGac                                     <  4:148302/123‑1 (MQ=255)
                                                                                                                                    gcaaCTTCAGGAGAACGGCCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGac                                     >  3:148302/4‑126 (MQ=255)
                                                                                                                                         ttCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGCACACACACAAATGCCACCgg          >  4:182760/1‑148 (MQ=255)
                                                                                                                                                 gCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGAccacca                                <  4:269437/118‑1 (MQ=255)
                                                                                                                                                 gCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGAccacca                                >  3:269437/1‑118 (MQ=255)
                                                                                                                                                 gCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGCACACACACAAATGCCACCGGCATCGCCa  >  1:109766/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCCAGCCTGACCAAACCGGATGATCCCGCGTTCCGCGCTGCTATCGAAAAGGCCATTGCCTCACAGCCGTGACCAGGCTGCGCTTGCTGCGGCGTTGCTTTAAAGCGCGAGGCAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGTTCTCCTGAAGTTGCGGGATCAAGATCAAGCACAGGAAGTGGCTGGCTGCGGGGCACTTCACCGTGCATGCACGGTAATAGCCACCAACCGCACCACCACCGGCCGGACCACCAGCACACACACAAATGCCACCGGCATCGCCA  >  NC_002947/1879745‑1880037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑