Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,882,347 C→T 8.3% A73T (GCT→ACT)  PP_1689 ← long‑chain fatty acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,882,3470CT 8.3% 136.1 / 2.4 48A73T (GCT→ACT) PP_1689long‑chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

AGATACCGAAACCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAGCGCGAAGCCGCT  >  NC_002947/1882200‑1882494
                                                                                                                                                   |                                                                                                                                                   
aGATACCGAAACCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAgc                                                                                                                                                     <  8:158260/148‑1 (MQ=255)
    aCCGAAACCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGcgccgc                                                                                                                                                >  6:176677/1‑149 (MQ=255)
      cGAAACCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCgccgcc                                                                                                                                               >  6:149100/1‑148 (MQ=255)
        aaaCCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCAc                                                                                                                                            <  7:100789/149‑1 (MQ=255)
                    cAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTc                                                                                                                                >  8:111709/1‑149 (MQ=255)
                    cAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTc                                                                                                                                <  5:315763/149‑1 (MQ=255)
                          tCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCtt                                                                                                                          >  8:6070/1‑149 (MQ=255)
                             tcgatcgGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAg                                                                                                                       >  5:215297/1‑149 (MQ=255)
                                 tcgGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCgg                                                                                                                   <  6:25365/149‑1 (MQ=255)
                                        tACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTGACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTGGGTTTTGGCGTTGACGGAAGCGCGGCCCAGGGTGAATTGTTCGGGGTTGTGGCGGGACATgc                                                                                                            >  7:79384/1‑149 (MQ=255)
                                                  cccATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGtt                                                                                                  <  6:215297/149‑1 (MQ=255)
                                                    cATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGc                                                                                                <  2:159618/149‑1 (MQ=255)
                                                    cATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCACCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGc                                                                                                >  2:161498/1‑149 (MQ=255)
                                                     aTGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGcc                                                                                               <  5:176677/149‑1 (MQ=255)
                                                        ggTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGTGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGaa                                                                                            >  7:36766/1‑149 (MQ=255)
                                                        ggTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGTGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGaa                                                                                            >  7:41735/1‑149 (MQ=255)
                                                          tACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATa                                                                                          <  7:111709/149‑1 (MQ=255)
                                                          tACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATa                                                                                          >  3:232379/1‑149 (MQ=255)
                                                                 accacacGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATCTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCTGGACATGCCGGCCGGGTTGCCGAATACGGTACt                                                                                   <  8:79384/146‑1 (MQ=255)
                                                                        gCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGcc                                                                                               >  8:58542/1‑130 (MQ=255)
                                                                        gCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGcc                                                                                               <  7:58542/130‑1 (MQ=255)
                                                                          aCCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTc                                                                          >  7:190978/1‑149 (MQ=255)
                                                                             aTGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTCGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCgg                                                                        >  2:131304/1‑148 (MQ=255)
                                                                                tCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCaga                                                                    <  8:190978/149‑1 (MQ=255)
                                                                                   ccATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGga                                                                 <  1:149015/149‑1 (MQ=255)
                                                                                   ccATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGga                                                                 <  1:161498/149‑1 (MQ=255)
                                                                                    cATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAgg                                                                  >  3:162970/1‑147 (MQ=255)
                                                                                     aTCGGTTTCACCCCCGAGGTTGCTGCCTGGGCCAGATATGTCGGTTTTCGCGATGACGGCACCGCCGCCCACGGTGAATTGATCGCGCTTGAGGCGGTACATTACGGCCGGGTAGCCGAATACGGTACTTGCATATTCGGCAGAgg                                                                  >  4:196358/1‑146 (MQ=255)
                                                                                         gTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCag                                                                     <  2:29370/139‑1 (MQ=255)
                                                                                         gTTTCCCCCCCGAGGTTGCTGCCTGGGCCCCATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCag                                                                     >  1:29370/1‑139 (MQ=255)
                                                                                                ccccGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCcgc                                                     >  7:58411/1‑148 (MQ=255)
                                                                                                  ccGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGa                                                   >  2:103973/1‑148 (MQ=255)
                                                                                                    gAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGTGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAAc                                                <  8:41735/149‑1 (MQ=255)
                                                                                                    gAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGTGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAAc                                                <  8:36766/149‑1 (MQ=255)
                                                                                                     aGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAAcc                                               <  4:232379/149‑1 (MQ=255)
                                                                                                     aGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAAcc                                               <  5:149100/149‑1 (MQ=255)
                                                                                                        ttGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCcgc                                                     >  2:68883/1‑140 (MQ=255)
                                                                                                        ttGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCcgc                                                     <  1:68883/140‑1 (MQ=255)
                                                                                                              ccTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCaga                                                                    <  1:11294/119‑1 (MQ=255)
                                                                                                              ccTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCaga                                                                    >  2:11294/1‑119 (MQ=255)
                                                                                                                  ggCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCAtgctg                                  >  2:134223/1‑149 (MQ=255)
                                                                                                                       gATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGAt                             >  4:41981/1‑149 (MQ=255)
                                                                                                                           tGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGctc                         >  3:292392/1‑149 (MQ=255)
                                                                                                                           tGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGct                          <  4:162970/148‑1 (MQ=255)
                                                                                                                              cGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGt                      >  6:75019/1‑149 (MQ=255)
                                                                                                                                gtgttGGAGATGACGGCAGCGCCGCCCACTGTTCATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGATCGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTc                    <  4:217099/146‑1 (MQ=255)
                                                                                                                                gTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTc                    >  8:191541/1‑149 (MQ=255)
                                                                                                                                       cGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAgc             <  4:292392/149‑1 (MQ=255)
                                                                                                                                               gCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAGCGCGAAGcc     >  6:299281/1‑149 (MQ=255)
                                                                                                                                                cAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAGCGCGAAGCCg    <  1:131304/149‑1 (MQ=255)
                                                                                                                                                  gcgcCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAGCGCGAAGCCGCt  <  8:58411/149‑1 (MQ=255)
                                                                                                                                                   cgccgcCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTccc                                                       >  1:57402/1‑95 (MQ=255)
                                                                                                                                                   cgccgcCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTccc                                                       <  2:57402/95‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGATACCGAAACCGACGCTCCAGTGGTCATCGATCGGTTTTACGTAGTAACCCATGGGTACGCCAACGACAGGCACCATGTCACCATCGGTTTCCCCCCCGAGGTTGCTGCCTGGGCCCGATATGTCGGTTTTGGCGATGACGGCAGCGCCGCCCACGGTGAATTGTTCGCGCTTGAGGCGGGACATGCCGGCCGGGTTGCCGAATACGGTACTGGCATCTTCGGCAGAGGAAGAACGTCCCGCGAAACCTGTCCCCATGCTGCTGATGCTCTGTTCGTTGAGCGCGAAGCCGCT  >  NC_002947/1882200‑1882494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑