Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,916,385 G→C 12.5% intergenic (+87/‑28) PP_1715 → / → PP_1716 hypothetical protein/Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,916,3850GC12.5% 110.0 / 6.6 40intergenic (+87/‑28)PP_1715/PP_1716hypothetical protein/Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (17/18);  new base C (2/3);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TGCGCGTGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAG  >  NC_002947/1916237‑1916532
                                                                                                                                                    |                                                                                                                                                   
tGCGCGTGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGCCCTCGGAGTTGGTATTATAATTGGCATAAGGGTTATGCCTACGGATTACATg                                                                                                                                                     >  4:223018/1‑149 (MQ=255)
  cgcgTGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATg                                                                                                                                                     <  4:101633/147‑1 (MQ=255)
      tGGTCTAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTTCACAGACCTCGGAGTTGTTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTaa                                                                                                                                               <  1:27737/149‑1 (MQ=255)
           gAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCtt                                                                                                                                          >  6:293699/1‑149 (MQ=255)
           gAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCtt                                                                                                                                          <  3:260728/149‑1 (MQ=255)
            aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCttt                                                                                                                                         <  1:203100/149‑1 (MQ=255)
            aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCttt                                                                                                                                         <  4:266709/149‑1 (MQ=255)
            aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                         <  1:967/149‑4 (MQ=255)
                            cccGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTaa                                                                                                                         <  8:277122/149‑1 (MQ=255)
                             ccGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATg                                                                                                                                                     >  4:4853/1‑120 (MQ=255)
                             ccGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATg                                                                                                                                                     <  3:4853/120‑1 (MQ=255)
                               ggAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAggg                                                                                                                                 >  1:17609/1‑138 (MQ=255)
                               ggAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAggg                                                                                                                                 <  2:17609/138‑1 (MQ=255)
                                                 gcgcAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                         >  2:246097/1‑109 (MQ=255)
                                                 gcgcAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                         <  1:246097/112‑4 (MQ=255)
                                                     aacaaGTAATGAAACCGGCCTGAATCTGACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGa                                                                                                <  3:69467/149‑1 (MQ=255)
                                                              tGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAAcc                                                                                       >  5:52963/1‑149 (MQ=255)
                                                                aaaCCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                         >  6:89717/1‑94 (MQ=255)
                                                                aaaCCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                         <  5:89717/97‑4 (MQ=255)
                                                                               tcACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTc                                                                      >  4:56488/1‑149 (MQ=255)
                                                                                cACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGa                                                                                    <  2:300941/134‑1 (MQ=255)
                                                                                cACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGa                                                                                    >  1:300941/1‑134 (MQ=255)
                                                                                     cAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCa                                                                                          >  1:135122/1‑123 (MQ=255)
                                                                                     cAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCa                                                                                          <  2:135122/123‑1 (MQ=255)
                                                                                      aaCTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                 <  1:182672/147‑1 (MQ=255)
                                                                                      aaCTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                 >  5:189272/1‑147 (MQ=255)
                                                                                        cTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAg                                                              >  5:193070/1‑148 (MQ=255)
                                                                                        cTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAg                                                              <  6:193070/148‑1 (MQ=255)
                                                                                                    cGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCt                                                                   >  2:20922/1‑131 (MQ=255)
                                                                                                    cGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCt                                                                   <  1:20922/131‑1 (MQ=255)
                                                                                                     ggAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGtt                                                <  3:56488/149‑1 (MQ=255)
                                                                                                                    aTTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTgcagg                                 >  2:38736/1‑149 (MQ=255)
                                                                                                                     ttGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCGGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTgcaggg                                >  1:5489/1‑149 (MQ=255)
                                                                                                                        gCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                 >  7:47438/1‑113 (MQ=255)
                                                                                                                        gCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                 <  8:47438/113‑1 (MQ=255)
                                                                                                                              gggTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCGGCGCTGCAGGGCAGGCACCa                       <  6:52963/149‑1 (MQ=255)
                                                                                                                                     gCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCGGCAGGGCAGGCACCAGCCGAgg                >  3:197013/1‑149 (MQ=255)
                                                                                                                                        tACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCCGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAAAACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCgg             >  6:272381/1‑149 (MQ=255)
                                                                                                                                         aCGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGt            >  7:222111/1‑149 (MQ=255)
                                                                                                                                          cGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGtt           >  5:146968/1‑149 (MQ=255)
                                                                                                                                                aCATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAg      <  6:189272/148‑1 (MQ=255)
                                                                                                                                                 cATGCTGTAATCCTTTTTAAAGGGGTTTGTACTGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGt     <  8:222111/148‑1 (MQ=255)
                                                                                                                                                 cATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGt     >  6:2636/1‑148 (MQ=255)
                                                                                                                                                   tGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAg  <  5:2636/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCGCGTGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAG  >  NC_002947/1916237‑1916532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑