Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,916,386 C→A 12.5% intergenic (+88/‑27) PP_1715 → / → PP_1716 hypothetical protein/Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,916,3860CA12.5% 116.9 / 6.9 40intergenic (+88/‑27)PP_1715/PP_1716hypothetical protein/Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/18);  new base A (2/3);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAGGC  >  NC_002947/1916243‑1916534
                                                                                                                                               |                                                                                                                                                    
tGGTCTAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTTCACAGACCTCGGAGTTGTTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTaa                                                                                                                                                 <  1:27737/149‑1 (MQ=255)
     gAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCtt                                                                                                                                            >  6:293699/1‑149 (MQ=255)
     gAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCtt                                                                                                                                            <  3:260728/149‑1 (MQ=255)
      aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCttt                                                                                                                                           <  1:203100/149‑1 (MQ=255)
      aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCttt                                                                                                                                           <  4:266709/149‑1 (MQ=255)
      aGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                           <  1:967/149‑4 (MQ=255)
                      cccGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTaa                                                                                                                           <  8:277122/149‑1 (MQ=255)
                         ggAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAggg                                                                                                                                   >  1:17609/1‑138 (MQ=255)
                         ggAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAggg                                                                                                                                   <  2:17609/138‑1 (MQ=255)
                                           gcgcAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                           >  2:246097/1‑109 (MQ=255)
                                           gcgcAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                           <  1:246097/112‑4 (MQ=255)
                                               aacaaGTAATGAAACCGGCCTGAATCTGACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGa                                                                                                  <  3:69467/149‑1 (MQ=255)
                                                        tGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAAcc                                                                                         >  5:52963/1‑149 (MQ=255)
                                                          aaaCCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                           <  5:89717/97‑4 (MQ=255)
                                                          aaaCCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACAGCATGTAATccgta                                                                                                                                           >  6:89717/1‑94 (MQ=255)
                                                                         tcACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTc                                                                        >  4:56488/1‑149 (MQ=255)
                                                                          cACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGa                                                                                      >  1:300941/1‑134 (MQ=255)
                                                                          cACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGa                                                                                      <  2:300941/134‑1 (MQ=255)
                                                                               cAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCa                                                                                            <  2:135122/123‑1 (MQ=255)
                                                                               cAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCa                                                                                            >  1:135122/1‑123 (MQ=255)
                                                                                aaCTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                   <  1:182672/147‑1 (MQ=255)
                                                                                aaCTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                   >  5:189272/1‑147 (MQ=255)
                                                                                  cTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAg                                                                <  6:193070/148‑1 (MQ=255)
                                                                                  cTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAg                                                                >  5:193070/1‑148 (MQ=255)
                                                                                              cGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCt                                                                     <  1:20922/131‑1 (MQ=255)
                                                                                              cGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCt                                                                     >  2:20922/1‑131 (MQ=255)
                                                                                               ggAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGtt                                                  <  3:56488/149‑1 (MQ=255)
                                                                                                              aTTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTgcagg                                   >  2:38736/1‑149 (MQ=255)
                                                                                                               ttGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCGGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTgcaggg                                  >  1:5489/1‑149 (MQ=255)
                                                                                                                  gCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                   >  7:47438/1‑113 (MQ=255)
                                                                                                                  gCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTgg                                                                   <  8:47438/113‑1 (MQ=255)
                                                                                                                        gggTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCGGCGCTGCAGGGCAGGCACCa                         <  6:52963/149‑1 (MQ=255)
                                                                                                                               gCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCGGCAGGGCAGGCACCAGCCGAgg                  >  3:197013/1‑149 (MQ=255)
                                                                                                                                  tACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCCGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAAAACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCgg               >  6:272381/1‑149 (MQ=255)
                                                                                                                                   aCGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGt              >  7:222111/1‑149 (MQ=255)
                                                                                                                                    cGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGtt             >  5:146968/1‑149 (MQ=255)
                                                                                                                                          aCATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAg        <  6:189272/148‑1 (MQ=255)
                                                                                                                                           cATGCTGTAATCCTTTTTAAAGGGGTTTGTACTGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGt       <  8:222111/148‑1 (MQ=255)
                                                                                                                                           cATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGt       >  6:2636/1‑148 (MQ=255)
                                                                                                                                             tGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAg    <  5:2636/149‑1 (MQ=255)
                                                                                                                                               cTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGGTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAGgc  <  5:293699/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGGTCGAGCGCCTGCCGGCTACCCCGGAAGCGCTGCGGCTGGAGCGCAACAAGTAATGAAACCGGCCTGAATCTCACCTCAACTGCACAGACCTCGGAGTTGGTAATATAATTGGCATAAGGGTTATGCCTACGGATTACATGCTGTAATCCTTTTTAAAGGGGTTTGTAATGGGTATTCAGGTCATCAGCCGGGACGGTCAACCCGAGTACGCCGTAGTTCCCTGGGAGCAATACCAGGCGTTGCTCAGCGCTGCAGGGCAGGCACCAGCCGAGGCGGTTTCCAGTGAGGC  >  NC_002947/1916243‑1916534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑