Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,928,266 A→G 8.0% F609S (TTC→TCC)  PP_1728 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,928,2660AG 8.0% 141.1 / 2.7 50F609S (TTC→TCC) PP_1728membrane protein
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base G (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACTCGAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGCCGAGGTGGCCGCCGACATCCAC  >  NC_002947/1928123‑1928414
                                                                                                                                               |                                                                                                                                                    
aCTCGAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGa                                                                                                                                                   <  1:96803/147‑1 (MQ=255)
  tCGAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATcgc                                                                                                                                               >  2:15112/1‑149 (MQ=255)
    gAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATcgcg                                                                                                                                              >  4:160869/1‑148 (MQ=255)
    gAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATcgc                                                                                                                                               <  8:191421/147‑1 (MQ=255)
    gAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGt                                                                                                                                             <  1:219301/149‑1 (MQ=255)
     aaGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGGAGTTcggcta                                                                                                                                            >  7:90198/1‑145 (MQ=255)
     aaGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGGAGTTcggcta                                                                                                                                            >  7:94506/1‑145 (MQ=255)
     aaGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTa                                                                                                                                            <  6:130491/149‑1 (MQ=255)
               cccccTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTcc                                                                                                                                  <  3:160869/149‑1 (MQ=255)
                  ccTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCcgc                                                                                                                               <  8:151351/149‑1 (MQ=255)
                   cTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATcgcg                                                                                                                                              >  1:134726/1‑133 (MQ=255)
                   cTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATcgcg                                                                                                                                              <  2:134726/133‑1 (MQ=255)
                      cAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGGAGTTCGGCTACGCGATCTTCcgcgtg                                                                                                                            <  8:90198/148‑3 (MQ=255)
                      cAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGGAGTTCGGCTACGCGATCTTCcgcgtg                                                                                                                            <  8:94506/148‑3 (MQ=255)
                      cAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGa                                                                                                                             <  3:52326/147‑1 (MQ=255)
                            gAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCt                                                                                                                     <  8:161777/149‑1 (MQ=255)
                             agctgataCCAGATATTACGTCTCTTCAGCTGGTCGTTGCGCAGCTTCTTCCCCAATTCGCGAACCAGCGAGATCGCATTGTAGATGTTCATGCTGTTCGTTATTGCCAAGCGGAAGATCGAGTAGCCTAACTACATCGAGTAGTTCt                                                                                                                     <  8:250190/141‑1 (MQ=255)
                             aGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACGAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCtt                                                                                                                    <  6:48267/149‑1 (MQ=255)
                             aGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGagta                                                                                                                          <  2:226420/143‑1 (MQ=255)
                             aGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGagta                                                                                                                          >  1:226420/1‑143 (MQ=255)
                                     ccatttatTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGctct                                                                                                            <  2:266746/144‑1 (MQ=255)
                                     ccAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGctct                                                                                                            <  1:15112/149‑1 (MQ=255)
                                      cAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCtt                                                                                                           <  7:82749/149‑1 (MQ=255)
                                        gATATTACGGCGCATCAGCGGGTCGTCGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCtt                                                                                                                    <  6:91099/138‑1 (MQ=255)
                                        gATATTACGGCGCATCAGCGGGTCGTCGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCtt                                                                                                                    >  5:91099/1‑138 (MQ=255)
                                              aCGGCGCATCAGCGGGTCGTTCCGCAGCATCACCCACACTTCGCCAACCAGCCAGATCTCCTGCTCGATGTTCATCATGTGCGCGAATTCCACGCGGAAGATCGCCTAGCCGAACTCCCGACACTAGTTCTTAATGCTCTTGATTTCga                                                                                                   >  6:73339/1‑148 (MQ=255)
                                                   gcATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTc                                                                                                     <  5:228592/142‑1 (MQ=255)
                                                   gcATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTc                                                                                                     >  6:228592/1‑142 (MQ=255)
                                                         gCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGa                                                                                          >  7:157608/1‑147 (MQ=255)
                                                                     gcAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTAt                                                                                    >  6:77132/1‑141 (MQ=255)
                                                                     gcAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTAt                                                                                    <  5:77132/141‑1 (MQ=255)
                                                                     gcAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATg                                                                            >  2:19596/1‑149 (MQ=255)
                                                                               ccccACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATg                                                                   <  2:294681/148‑1 (MQ=255)
                                                                               ccccACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATAGTATGCACGATGCCGTCGATGt                                                                  <  8:157608/149‑1 (MQ=255)
                                                                                cccACTTCGCGAACCAGCGAGAACGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTc                                                                 >  3:75536/1‑149 (MQ=255)
                                                                                 ccACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGc                                                                                  <  5:282804/131‑1 (MQ=255)
                                                                                 ccACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGc                                                                                  >  6:282804/1‑131 (MQ=255)
                                                                                   aCTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGACGATGTcgc                                                               >  7:288167/1‑148 (MQ=255)
                                                                                          gAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGgcg                                                        >  6:165789/1‑148 (MQ=255)
                                                                                                  gagaTCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTAc                                                >  2:194791/1‑148 (MQ=255)
                                                                                                      tCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATg                                           >  3:1706/1‑149 (MQ=255)
                                                                                                      tCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATg                                           >  1:68608/1‑149 (MQ=255)
                                                                                                         ccTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGt                                         >  3:62602/1‑148 (MQ=255)
                                                                                                              tCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAAccc                                   >  3:43315/1‑149 (MQ=255)
                                                                                                                gATGTTCATGCTGTTCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAAAGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCt                                  <  5:46917/148‑1 (MQ=255)
                                                                                                                gATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCtt                                 >  4:15620/1‑149 (MQ=255)
                                                                                                                    ttCCTGCTGTGCGGTTTTGCCAGGCTGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGATCGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCAc                             <  8:288167/149‑1 (MQ=255)
                                                                                                                         gCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGc                        >  6:270379/1‑149 (MQ=255)
                                                                                                                          cTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGc                        <  1:19596/148‑1 (MQ=255)
                                                                                                                             tgCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGCCGa                     <  2:108154/148‑1 (MQ=255)
                                                                                                                                               aaGATCGCGTAGCCGAACTCGCGCGAGTAGATCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGCCGAGGTGGCCGCCGACATCcac  <  5:73339/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
ACTCGAAGCTCTCCACCCCCTGCAGCTCGAGCGGCGACCAGATATTACGGCGCATCAGCGGGTCGTTGCGCAGCTTCTGCCCCACTTCGCGAACCAGCGAGATCGCCTGGTCGATGTTCATGCTGTGCGGGATTGCCACGCGGAAGATCGCGTAGCCGAACTCCCGCGAGTAGTTCTTGATGCTCTTGATTTCGCTGAACGGGATGGTATGCACGATGCCGTCGATGTCGCGCAGGCGCACGGTACGAATGGTCAACCCTTCCACCGTGCCGAGGTGGCCGCCGACATCCAC  >  NC_002947/1928123‑1928414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑