| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,933,882 | A→G | 9.3% | L270S (TTG→TCG) ‡ | minC ← | septum site‑determining protein MinC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,933,882 | 0 | A | G | 9.3% | 112.6 / 2.8 | 43 | L270S (TTG→TCG) ‡ | minC | septum site‑determining protein MinC |
| Reads supporting (aligned to +/- strand): ref base A (17/22); new base G (2/2); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGA > NC_002947/1933735‑1934029 | ccACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCaa > 2:60612/1‑149 (MQ=255) cGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAacac < 8:205493/149‑1 (MQ=255) agaATCTTGGCCAAGGTGTTTCACCCCTAAAACTAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGt < 8:157609/149‑1 (MQ=255) tCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTGCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCAc > 1:224495/1‑148 (MQ=255) tCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCAcc > 4:19710/1‑149 (MQ=255) ggCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATGCCTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCAc < 1:60612/145‑1 (MQ=255) aaGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGc > 8:151764/1‑149 (MQ=255) aGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCt < 3:104917/149‑1 (MQ=255) aGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCt < 8:19015/149‑1 (MQ=255) cccTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGc > 8:141045/1‑149 (MQ=255) cccTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGc > 6:71898/1‑149 (MQ=255) cTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTg < 5:99786/149‑1 (MQ=255) cTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTg < 7:194672/149‑1 (MQ=255) cTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTg < 7:141045/149‑1 (MQ=255) aaaTAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTcc > 5:213227/1‑149 (MQ=255) aaaTAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTcc < 1:132064/149‑1 (MQ=255) aTAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAAGAAAAGTTGCTCTAAAAATG‑CTGGCAAGTATAAGTTAAAGACTGGTGATGTTAAAAACGTCACCAGACAGGCTTACTTGAACGATGGTcac > 1:248984/1‑147 (MQ=255) gggACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTTGacac < 6:222700/109‑1 (MQ=255) gggACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTTGacac < 6:222697/109‑1 (MQ=255) gggACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTTGacac > 5:222697/1‑109 (MQ=255) gggACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGATGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTTGacac > 5:222700/1‑109 (MQ=255) ggACACTTGTACCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCg < 3:103311/149‑1 (MQ=255) ttGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATccc > 1:146593/1‑149 (MQ=255) cTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGgcg > 4:203574/1‑147 (MQ=255) aaaaCTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCt > 4:45684/1‑148 (MQ=255) aaaCTGAAATATCTAAATTAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGAAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTc < 4:161691/149‑1 (MQ=255) aaaCTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTc < 7:151764/149‑1 (MQ=255) aCGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATc < 2:224495/149‑1 (MQ=255) ggTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATc > 3:120493/1‑147 (MQ=255) ggTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCga < 6:213227/149‑1 (MQ=255) aaaaGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCa < 5:71898/149‑1 (MQ=255) aaaaGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCa > 1:140360/1‑149 (MQ=255) aaaGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCgg < 1:275673/136‑1 (MQ=255) aaaGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCgg > 2:275673/1‑136 (MQ=255) tAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTcggcgg < 2:248984/147‑1 (MQ=255) aaaaTG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTTCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTc < 3:45684/147‑1 (MQ=255) aaaaTG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCAt < 2:140360/149‑1 (MQ=255) tGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTgg > 8:67718/1‑149 (MQ=255) aaGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCgg < 7:254195/113‑1 (MQ=255) aaGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCgg > 8:254195/1‑113 (MQ=255) ccGTTAAAGACGGGTGATGTTCAACACGACACCAGACAGGCTGACATGCACGCTCGTCCACCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTAGTACTGGCAGCCGATCGAGACCATTTCCGCGCTCATCTGCTGGCAGAAGATAc > 2:10392/1‑149 (MQ=255) cGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGGACGCTGGTCCCCCACATCGGATCCGGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGgt < 1:10392/149‑1 (MQ=255) aTGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGc < 3:203574/148‑1 (MQ=255) tCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCttt > 6:143653/1‑148 (MQ=255) aaCACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGa < 2:146593/148‑1 (MQ=255) | CCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGGCAACGGTAA‑AAAAGTTGCTCTAAAAATG‑CTGGCAAGTATCCGTTAAAGACGGGTGATGTTCAACACGTCACCAGACAGGCTGACTTGCACGCTGGTCCCCCACAGCGGATCCCGGCGCAGGTCTTCGCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGA > NC_002947/1933735‑1934029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |