| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,021,487 | G→C | 11.4% | intergenic (+24/‑415) | PP_1798 → / → gmd | outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,021,487 | 0 | G | C | 11.4% | 105.7 / 8.3 | 44 | intergenic (+24/‑415) | PP_1798/gmd | outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (20/19); new base C (3/2); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-02 | |||||||||||
TCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTA > NC_002947/2021342‑2021635 | tCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGTCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGc > 2:9486/1‑149 (MQ=255) aaCCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCt > 2:95560/1‑148 (MQ=255) aaCCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTg < 5:59529/149‑1 (MQ=255) ccGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGaa < 4:134970/149‑1 (MQ=255) gTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagc > 1:254401/1‑149 (MQ=255) gggCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcgc < 4:167199/148‑1 (MQ=255) gggTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGATGAagagaagcga > 2:317323/1‑148 (MQ=255) ggAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCACCCAGTGAAgcg > 6:113772/1‑148 (MQ=255) aaGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGcaca < 8:175576/149‑1 (MQ=255) aaGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGcaca < 5:48201/149‑1 (MQ=255) tgtgGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTa > 6:174089/1‑149 (MQ=255) tAACCTCAATGTCTGGTTGAACCCGGAGCCTGAGACCAGCCTGCCCGGTAGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCCCAGCGCCAACCAGTGAAGCGCACAGTATCAAtt > 2:42438/1‑149 (MQ=255) gTCAATGTCTGGTTGAGCCCGGTGCCTGAGNCCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc > 1:28062/1‑149 (MQ=255) gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc < 2:254401/149‑1 (MQ=255) gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc > 7:150459/1‑149 (MQ=255) gTTGAGCCCGGTGCCTGAGACCAGCCTGTCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTccc < 1:9486/147‑1 (MQ=255) gTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcg < 4:259902/101‑1 (MQ=255) gTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcg > 3:259902/1‑101 (MQ=255) gAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTg > 4:113082/1‑149 (MQ=255) gCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGc > 6:153049/1‑148 (MQ=255) gCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGc < 5:153049/148‑1 (MQ=255) cccGGTGCCTGAGACCAGCCTGCCCGGGGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTGCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGt < 5:263455/149‑1 (MQ=255) cTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTcc > 7:142352/1‑149 (MQ=255) aCCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTg > 6:145145/1‑149 (MQ=255) ctgCCTGCCCGGTGGCGAGGTTGGTCGCGTGGGCGCCAGTCGCTGATAGCGAGGGGGTCTGCGGTTCAGGGGCTGAAGCGCAGCTCCAACCAGTGAAGCTCACAGTATCAATTTCAGCCCCGGTCCCTTTTGCGTCCTGTTCCCAtttc < 1:42438/147‑3 (MQ=255) tGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCTATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCgtgt > 8:140841/1‑147 (MQ=255) cGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGc > 4:245574/1‑148 (MQ=255) ggTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa < 3:113082/149‑1 (MQ=255) gAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa > 1:12767/1‑143 (MQ=255) gAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa < 2:12767/143‑1 (MQ=255) tCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGttt > 6:173173/1‑149 (MQ=255) ccTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGc < 5:174089/149‑1 (MQ=255) gTCGCTGAAAGCGAGGGGGTCTCCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCt > 6:33564/1‑149 (MQ=255) tGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGAc < 4:69964/142‑1 (MQ=255) tGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGAc > 3:69964/1‑142 (MQ=255) gAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAgcg > 3:294845/1‑148 (MQ=255) aaaGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGATCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAgcgc < 7:289725/148‑1 (MQ=255) gCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGc < 5:33564/149‑1 (MQ=255) gCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGc < 3:245574/149‑1 (MQ=255) gggTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGAccc < 8:150459/149‑1 (MQ=255) gcTGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCggg > 5:24188/2‑149 (MQ=255) tGCGCTTCAGCCCCTGAAGCGCAACGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCg > 7:197758/1‑149 (MQ=255) cgcTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGc < 8:142352/146‑1 (MQ=255) cgcTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGt < 1:95560/149‑1 (MQ=255) ggggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgctgc > 5:27290/1‑148 (MQ=255) ggggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgctgc > 2:54229/1‑148 (MQ=255) gggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTa > 1:294892/1‑149 (MQ=255) | TCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTA > NC_002947/2021342‑2021635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |