Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,021,489	G→C	11.0%	intergenic (+26/‑413)	PP_1798 → / → gmd	outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,021,489	0	G	C	11.0%	125.9 / 5.9	44	intergenic (+26/‑413)	PP_1798/gmd	outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base C (3/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

TCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATG  >  NC_002947/2021342‑2021637                                                                                                                                                    |                                                                                                                                                      tCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGTCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGc                                                                                                                                                      >  2:9486/1‑149 (MQ=255)   aaCCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCt                                                                                                                                                     >  2:95560/1‑148 (MQ=255)   aaCCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTg                                                                                                                                                    <  5:59529/149‑1 (MQ=255)     ccGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGaa                                                                                                                                                  <  4:134970/149‑1 (MQ=255)       gTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagc                                                                                                                                                >  1:254401/1‑149 (MQ=255)          gggCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcgc                                                                                                                                              <  4:167199/148‑1 (MQ=255)              gggTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGATGAagagaagcga                                                                                                                                         >  2:317323/1‑148 (MQ=255)                             ggAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCACCCAGTGAAgcg                                                                                                                           >  6:113772/1‑148 (MQ=255)                                aaGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGcaca                                                                                                                       <  8:175576/149‑1 (MQ=255)                                aaGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGcaca                                                                                                                       <  5:48201/149‑1 (MQ=255)                                   tgtgGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTa                                                                                                                    >  6:174089/1‑149 (MQ=255)                                         tAACCTCAATGTCTGGTTGAACCCGGAGCCTGAGACCAGCCTGCCCGGTAGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCCCAGCGCCAACCAGTGAAGCGCACAGTATCAAtt                                                                                                              >  2:42438/1‑149 (MQ=255)                                             gTCAATGTCTGGTTGAGCCCGGTGCCTGAGNCCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc                                                                                                         >  1:28062/1‑149 (MQ=255)                                             gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc                                                                                                         <  2:254401/149‑1 (MQ=255)                                             gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGc                                                                                                         >  7:150459/1‑149 (MQ=255)                                                        gTTGAGCCCGGTGCCTGAGACCAGCCTGTCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTccc                                                                                                <  1:9486/147‑1 (MQ=255)                                                        gTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcg                                                                                                                                               <  4:259902/101‑1 (MQ=255)                                                        gTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGCCCCTGAagcg                                                                                                                                               >  3:259902/1‑101 (MQ=255)                                                           gAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTg                                                                                           >  4:113082/1‑149 (MQ=255)                                                             gCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGc                                                                                          >  6:153049/1‑148 (MQ=255)                                                             gCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGc                                                                                          <  5:153049/148‑1 (MQ=255)                                                              cccGGTGCCTGAGACCAGCCTGCCCGGGGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTGCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGt                                                                                        <  5:263455/149‑1 (MQ=255)                                                                      cTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTcc                                                                                >  7:142352/1‑149 (MQ=255)                                                                           aCCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTg                                                                           >  6:145145/1‑149 (MQ=255)                                                                             ctgCCTGCCCGGTGGCGAGGTTGGTCGCGTGGGCGCCAGTCGCTGATAGCGAGGGGGTCTGCGGTTCAGGGGCTGAAGCGCAGCTCCAACCAGTGAAGCTCACAGTATCAATTTCAGCCCCGGTCCCTTTTGCGTCCTGTTCCCAtttc                                                                          <  1:42438/147‑3 (MQ=255)                                                                                  tGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCTATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCgtgt                                                                      >  8:140841/1‑147 (MQ=255)                                                                                      cGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGc                                                                 >  4:245574/1‑148 (MQ=255)                                                                                       ggTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa                                                               <  3:113082/149‑1 (MQ=255)                                                                                             gAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa                                                               >  1:12767/1‑143 (MQ=255)                                                                                             gAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGa                                                               <  2:12767/143‑1 (MQ=255)                                                                                                     tCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGttt                                                 >  6:173173/1‑149 (MQ=255)                                                                                                        ccTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGc                                              <  5:174089/149‑1 (MQ=255)                                                                                                                   gTCGCTGAAAGCGAGGGGGTCTCCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCt                                   >  6:33564/1‑149 (MQ=255)                                                                                                                        tGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGAc                                     >  3:69964/1‑142 (MQ=255)                                                                                                                        tGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGAc                                     <  4:69964/142‑1 (MQ=255)                                                                                                                         gAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAgcg                              >  3:294845/1‑148 (MQ=255)                                                                                                                          aaaGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGATCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAgcgc                             <  7:289725/148‑1 (MQ=255)                                                                                                                             gCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGc                         <  5:33564/149‑1 (MQ=255)                                                                                                                             gCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGc                         <  3:245574/149‑1 (MQ=255)                                                                                                                                   gggTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGAccc                   <  8:150459/149‑1 (MQ=255)                                                                                                                                      gcTGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCggg                >  5:24188/2‑149 (MQ=255)                                                                                                                                        tGCGCTTCAGCCCCTGAAGCGCAACGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCg              >  7:197758/1‑149 (MQ=255)                                                                                                                                          cgcTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGc               <  8:142352/146‑1 (MQ=255)                                                                                                                                          cgcTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGt            <  1:95560/149‑1 (MQ=255)                                                                                                                                                 ggggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgctgc      >  5:27290/1‑148 (MQ=255)                                                                                                                                                 ggggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgctgc      >  2:54229/1‑148 (MQ=255)                                                                                                                                                  gggCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTa    >  1:294892/1‑149 (MQ=255)                                                                                                                                                    gCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATg  <  5:145145/149‑1 (MQ=255)                                                                                                                                                    gCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATg  <  2:28062/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTT‑AGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATG  >  NC_002947/2021342‑2021637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑