| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,052,784 | G→T | 8.8% | intergenic (+16/+34) | prmB → / ← PP_1828 | N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,052,784 | 0 | G | T | 8.8% | 175.7 / 6.5 | 57 | intergenic (+16/+34) | prmB/PP_1828 | N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/27); new base T (3/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CATGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTC > NC_002947/2052640‑2052931 | cATGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGc < 4:172177/149‑1 (MQ=255) gTCGAGGCGTTGTACCAGGAGGTGGACTTTGACTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCACGAATTCTTCAAGGCCCACGTTGAGGCTATTTGGGGCCGACAAGCGGc > 2:286079/1‑149 (MQ=255) tCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGc < 4:222048/148‑1 (MQ=255) tCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGc < 3:129608/148‑1 (MQ=255) gCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAAt < 3:155935/149‑1 (MQ=255) gTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTAGGGCCGCTTCGCGGCCCCaaata < 2:60312/149‑4 (MQ=255) gTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCACTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTacc > 1:186644/1‑149 (MQ=255) ccGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTcc < 5:174372/143‑1 (MQ=255) ccGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTcc > 6:174372/1‑143 (MQ=255) cGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCt > 4:206572/1‑149 (MQ=255) cTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTc > 4:14907/1‑132 (MQ=255) cTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTc < 3:14907/132‑1 (MQ=255) ctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGAt < 4:34743/133‑1 (MQ=255) ctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGAt > 3:34743/1‑133 (MQ=255) ctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTa < 4:156089/149‑1 (MQ=255) ctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTa < 3:206572/149‑1 (MQ=255) tggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAg > 6:172823/1‑149 (MQ=255) gAGTTCGTGCGGGGCGGGCATGGGGTGTTCATGCTCACCTCCGAGCAGTGCAGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCTCTTAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCa < 1:286079/149‑1 (MQ=255) aGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCaa < 4:240969/149‑1 (MQ=255) gTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAAt > 2:60780/1‑149 (MQ=255) cGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATc > 5:224889/1‑147 (MQ=255) cGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGCCTGATGA‑GGTCTTAGCGGGTAGCAATc > 6:33037/1‑147 (MQ=255) gAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAg > 6:59594/1‑141 (MQ=255) gAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAg < 5:59594/141‑1 (MQ=255) gAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATc > 4:143634/1‑146 (MQ=255) aGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATc > 3:279382/1‑145 (MQ=255) gctgggcgggcATGGTGTGTTCATGCTCACCGCCGAGCAGTGACGCCATCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAATCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGAt < 2:186644/146‑1 (MQ=255) gggcATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGAt < 3:114538/142‑1 (MQ=255) gggcATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGAt > 4:114538/1‑142 (MQ=255) tGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATTAACAGCcctgc < 4:54783/149‑1 (MQ=255) gtgtTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcctg > 3:223596/1‑148 (MQ=255) ttCATGCTCACCGCCGATCAGTGCCGCCAGCATGAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGaa < 1:60780/149‑1 (MQ=255) aTGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGc > 3:191060/1‑77 (MQ=255) aTGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGc < 4:191060/77‑1 (MQ=255) cTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑g < 6:20746/100‑1 (MQ=255) cTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑g > 5:20746/1‑100 (MQ=255) tCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCa > 5:219515/1‑148 (MQ=255) cgccgAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGc > 3:75768/1‑149 (MQ=255) cgAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGccac < 6:224889/149‑1 (MQ=255) aGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCACACGcccc > 6:64511/1‑145 (MQ=255) gTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACcaggca < 6:219515/149‑1 (MQ=255) ccAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAAt < 3:143634/149‑1 (MQ=255) cATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcct < 2:254026/114‑1 (MQ=255) cATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcct > 1:254026/1‑114 (MQ=255) tCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGa > 4:11295/1‑148 (MQ=255) aGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGaaa < 1:15464/148‑1 (MQ=255) ggAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCACCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACa > 5:26816/1‑100 (MQ=255) gAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGc < 5:172823/149‑1 (MQ=255) ttCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATccg > 8:225546/1‑149 (MQ=255) ccTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCTGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCGATCCGCCGTCTTc > 4:118268/1‑149 (MQ=255) gaaattagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAtt > 1:312818/8‑149 (MQ=255) cTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAtt < 4:75768/149‑1 (MQ=255) tttGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTgg < 3:11295/149‑1 (MQ=255) tttGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTg < 4:223596/148‑1 (MQ=255) attagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTgg > 5:173701/5‑149 (MQ=255) ttagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGc > 6:305835/4‑149 (MQ=255) gggCCGCGAAGCGTCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTAc < 7:225546/149‑1 (MQ=255) cgAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTTCCTGGAAAACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTc < 5:33037/149‑1 (MQ=255) | CATGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGC‑GGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTACGCGCTC > NC_002947/2052640‑2052931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |