| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,104,902 | G→C | 15.5% | intergenic (‑19/+22) | PP_1879 ← / ← PP_1880 | hypothetical protein/outer membrane autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,104,902 | 0 | G | C | 15.5% | 95.5 / 8.8 | 38 | intergenic (‑19/+22) | PP_1879/PP_1880 | hypothetical protein/outer membrane autotransporter |
| Reads supporting (aligned to +/- strand): ref base G (17/15); new base C (2/4); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACC > NC_002947/2104761‑2105046 | aTCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTGGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGc < 5:246564/149‑1 (MQ=255) tCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCCAATGCGCCTTc < 4:164806/149‑1 (MQ=255) ccacGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCCAATGCGCCTTCCCtt < 5:47846/149‑2 (MQ=255) acGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCCAATGCGCCTTCCCttct < 5:104643/149‑4 (MQ=255) ttGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTAGCGGAGCGCTTGTTCAGTGTGATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGCAGGCGCATTGGCCGTCCCTCGTTTCt > 4:195321/1‑149 (MQ=255) ggTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCtt < 6:266346/148‑1 (MQ=255) gagagGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCAATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGcc > 5:258345/1‑149 (MQ=255) cGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGgcgc > 1:75813/1‑141 (MQ=255) cGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGgcgc < 2:75813/141‑1 (MQ=255) tCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCa > 4:180320/1‑123 (MQ=255) tCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCa < 3:180320/123‑1 (MQ=255) tCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCt > 6:109153/1‑138 (MQ=255) tCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCt < 5:109153/138‑1 (MQ=255) gTTGGAGTTCTGAGGTTACCGGAGCGAGTGTTCTCTGTCATCGTAAATCTCCATGTGTCACGCATGAATGCAGAAGCGAACGCCCATTGGCCTTCCCTATGTTCTTTACCAGTTGTAACTACCGCCGACGCTCACGCCCCCTGACTGtg > 8:228119/1‑148 (MQ=255) gAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGGCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCaa < 8:162591/148‑1 (MQ=255) ttGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTc < 3:65314/106‑1 (MQ=255) ttGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTc > 4:65314/1‑106 (MQ=255) ttGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCg > 7:56245/1‑148 (MQ=255) tCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCCAATGCGCCTTccc > 6:138652/1‑66 (MQ=37) tCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCCAATGCGCCTTccc < 5:138652/66‑1 (MQ=37) tgtCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCg > 2:136926/1‑149 (MQ=255) aTCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTggg > 4:58226/1‑149 (MQ=255) cGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTtgctg < 4:213494/119‑1 (MQ=255) cGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTtgctg > 3:213494/1‑119 (MQ=255) cATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCAttt < 8:56245/147‑1 (MQ=255) aTGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTc < 8:54469/98‑1 (MQ=255) aTGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTc > 7:54469/1‑98 (MQ=255) aTGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTc < 6:258345/149‑1 (MQ=255) aCGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAg > 5:200195/1‑149 (MQ=255) aaTGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGc < 8:21517/144‑1 (MQ=255) aaTGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGc > 7:21517/1‑144 (MQ=255) aGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCAcc > 3:302484/1‑149 (MQ=255) gaagggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCAccc < 1:136926/149‑1 (MQ=255) aagggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTtgctg > 6:213775/1‑87 (MQ=255) aagggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTtgctg < 5:213775/87‑1 (MQ=255) gggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTg > 2:164659/1‑149 (MQ=255) ggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTg > 4:165431/1‑148 (MQ=255) aggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCAcc < 4:302484/149‑1 (MQ=255) | ATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACC > NC_002947/2104761‑2105046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |