Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,166,644	T→A	6.8%	intergenic (‑488/‑293)	PP_5483 ← / → PP_1921	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,166,644	0	T	A	6.8%	216.8 / 4.9	73	intergenic (‑488/‑293)	PP_5483/PP_1921	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base A (2/3);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CACTTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATT  >  NC_002947/2166496‑2166785                                                                                                                                                      |                                                                                                                                              cACTTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCt                                                                                                                                               <  6:182524/149‑1 (MQ=255) cACTTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCt                                                                                                                                               >  6:166944/1‑149 (MQ=255)  aCTTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGATTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTg                                                                                                                                              <  7:140063/149‑1 (MQ=255)           cccGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGga                                                                                                                                      <  3:3318/148‑1 (MQ=255)           cccGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGga                                                                                                                                      <  5:166944/148‑1 (MQ=255)           cccGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggtc                                                                                                                                     <  1:84333/149‑3 (MQ=255)             cGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggtcgt                                                                                                                                   <  7:157036/149‑5 (MQ=255)                   acgaTTCAGTGATTAGCTGGCAATGGCGGGTTAGTTGTCGGCATCTCGCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGa                                                                                                                              <  5:93201/145‑1 (MQ=255)                    gCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAgg                                                                                                                            <  4:167468/149‑1 (MQ=255)                     cATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCAGGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAgg                                                                                                                            >  1:86904/1‑149 (MQ=255)                     cATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggtcgttt                                                                                                                                 >  4:29699/1‑137 (MQ=255)                     cATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCAGGAGCgggtcgttt                                                                                                                                 <  3:29699/143‑7 (MQ=255)                           gTGATTAGCTGTCAATGGCGGGGGAGGTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTg                                                                                                                     <  4:257217/149‑1 (MQ=255)                                      gCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTg                                                                                                          >  5:113576/1‑149 (MQ=255)                                      gCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTg                                                                                                          >  6:27764/1‑149 (MQ=255)                                           ggCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAggg                                                                                                                           >  4:220201/1‑127 (MQ=255)                                                       gACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCtcaat                                                                                         >  5:106917/1‑149 (MQ=255)                                                                tcCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGa                                                                                                     >  8:197400/1‑128 (MQ=255)                                                                tcCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGa                                                                                                     <  7:197400/128‑1 (MQ=255)                                                                tcCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGc                                                                                >  5:6364/1‑149 (MQ=255)                                                                  ccTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCtcaatc                                                                                        >  6:135435/1‑139 (MQ=255)                                                                  ccTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCtcaatc                                                                                        <  5:135435/139‑1 (MQ=255)                                                                  ccTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGc                                                                                <  6:6364/147‑1 (MQ=255)                                                                  ccTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGc                                                                                <  3:281601/147‑1 (MQ=255)                                                                   cTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGtt                                                                                                       <  2:138349/123‑1 (MQ=255)                                                                   cTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGtt                                                                                                       >  1:138349/1‑123 (MQ=255)                                                                      gATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGGTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAg                                                                          >  1:218415/1‑149 (MQ=255)                                                                      gATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAg                                                                          >  4:231140/1‑149 (MQ=255)                                                                          a‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTg                                                                      <  5:27764/149‑1 (MQ=255)                                                                            gAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGTAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTAGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTg                                                                      >  3:100846/1‑148 (MQ=255)                                                                            gAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTg                                                                      >  7:178101/1‑148 (MQ=255)                                                                              aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgatt                                                                   <  2:86904/149‑4 (MQ=255)                                                                                gTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGt                                                                 >  8:292016/1‑149 (MQ=255)                                                                                gTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGt                                                                 >  6:253451/1‑149 (MQ=255)                                                                                   aCTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCg                                                               <  3:271461/148‑1 (MQ=255)                                                                                    ctctCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgta                                                             <  3:231140/149‑1 (MQ=255)                                                                                       tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGa                                                                                                                              >  6:95338/1‑81 (MQ=255)                                                                                       tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGa                                                                                                                              <  5:95338/81‑1 (MQ=255)                                                                                       tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg                                                           >  1:116899/1‑148 (MQ=255)                                                                                         aGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg                                                           <  7:292016/146‑1 (MQ=255)                                                                                         aGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCCCTCCTGGAGCGGGCTGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg                                                           <  1:98895/146‑1 (MQ=255)                                                                                             ataggttGCTGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggattagg                                                    <  7:156930/144‑1 (MQ=255)                                                                                             aCCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagg                                                    <  6:113576/149‑1 (MQ=255)                                                                                                tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCATGCAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggag                                                 >  5:223600/1‑149 (MQ=255)                                                                                                tATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCAGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCATGTCgtagggagtagggag                                                 >  7:128026/1‑149 (MQ=255)                                                                                                  ttGCAGAGTGTTTAAGGAGCGCCGGTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagta                                               <  6:223600/149‑1 (MQ=255)                                                                                                    gCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                             >  5:155203/1‑149 (MQ=255)                                                                                                       gagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggag                                          >  8:228950/1‑149 (MQ=255)                                                                                                       gagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggag                                          <  2:116899/149‑1 (MQ=255)                                                                                                       gagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgat                                                                    <  8:175554/123‑3 (MQ=255)                                                                                                       gagGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgat                                                                    >  7:175554/1‑121 (MQ=255)                                                                                                          ggTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtag                                       <  8:178101/149‑1 (MQ=255)                                                                                                              tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga                                    <  6:155203/148‑1 (MQ=255)                                                                                                                  gATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGgagtagggagtagggagtagggagtagg                               <  6:106917/149‑1 (MQ=255)                                                                                                                          gtGGCCATTCTGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGga                       <  2:218415/148‑1 (MQ=255)                                                                                                                             gCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGgaga                     <  5:253451/148‑1 (MQ=255)                                                                                                                               cATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGtagggagtagggagtagggagtagggag                                   >  5:196642/1‑132 (MQ=255)                                                                                                                               cATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGtagggagtagggagtagggagtagggag                                   <  6:196642/132‑1 (MQ=255)                                                                                                                                    tgtgTAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgcg              <  4:100846/148‑1 (MQ=255)                                                                                                                                    tgtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                             >  1:120483/1‑117 (MQ=255)                                                                                                                                    tgtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                             <  2:120483/117‑1 (MQ=255)                                                                                                                                     gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGgaga                     <  2:174768/140‑1 (MQ=255)                                                                                                                                     gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGgaga                     >  1:174768/1‑140 (MQ=255)                                                                                                                                     gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGga                       <  7:241976/138‑1 (MQ=255)                                                                                                                                     gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGga                       >  8:241976/1‑138 (MQ=255)                                                                                                                                      tgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgcgc             <  3:151425/147‑1 (MQ=255)                                                                                                                                      tgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgcgc             >  4:151425/1‑147 (MQ=255)                                                                                                                                       gAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCggg          >  4:207133/1‑149 (MQ=255)                                                                                                                                        aaaCGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCgg           <  2:4835/147‑1 (MQ=255)                                                                                                                                        aaaCGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCgg           >  1:4835/1‑147 (MQ=255)                                                                                                                                        aaaCGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGt         >  8:247121/1‑149 (MQ=255)                                                                                                                                         atcatcccGCTCCAGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTTGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTg        >  4:224751/6‑149 (MQ=255)                                                                                                                                             aCCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTg        >  1:36838/1‑145 (MQ=255)                                                                                                                                              cccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGAt   >  3:203044/1‑149 (MQ=255)                                                                                                                                               ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGTGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGAtt  >  6:199272/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                              CACTTTTTTCCCCGGGTGAGCATTCAGTGATTAGCTGGCAATGGCGGGTGAGTTGACGTCATCTCCCTCGATCA‑GAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATT  >  NC_002947/2166496‑2166785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑