Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,224,834 A→G 9.4% I600T (ATT→ACT)  PP_1963 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,224,8340AG 9.4% 173.4 / 7.2 64I600T (ATT→ACT) PP_1963hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base G (3/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTTT  >  NC_002947/2224689‑2224981
                                                                                                                                                 |                                                                                                                                                   
cGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCTCTTTCATTTTCAAGCTCATACTCAatat                                                                                                                                                  >  3:16964/1‑149 (MQ=255)
  gggCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAatat                                                                                                                                                  >  8:215810/1‑147 (MQ=255)
  gggCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGa                                                                                                                                                <  8:27459/149‑1 (MQ=255)
  cggCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGa                                                                                                                                                <  6:137078/148‑1 (MQ=255)
    gCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGc                                                                                                                                              <  8:208635/149‑1 (MQ=255)
     cGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCt                                                                                                                                             <  8:114115/149‑1 (MQ=255)
            ccgTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCt                                                                                                                                      >  8:62052/1‑149 (MQ=255)
               tCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCt                                                                                                                                             <  6:190951/139‑1 (MQ=255)
               tCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCt                                                                                                                                             >  5:190951/1‑139 (MQ=255)
                   tatCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCAt                                                                                                                                  <  3:192224/146‑1 (MQ=255)
                       gcgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACg                                                                                                                                        >  4:296785/1‑136 (MQ=255)
                       gcgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACg                                                                                                                                        <  3:296785/136‑1 (MQ=255)
                       gcgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACTCTCCATCAAATAg                                                                                                                           >  6:101372/1‑149 (MQ=255)
                       gcgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAg                                                                                                                           <  7:62052/149‑1 (MQ=255)
                        cgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAAATTTACCTTCCCAGCGCAGGTCAGAGGACCGTCCACCTACGCTTTCATTTTCAAGCTCATACTAAATATGAGCTAGACGCTCCATCAAAAAgg                                                                                                                          >  5:22391/1‑149 (MQ=255)
                         gAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAggg                                                                                                                         >  2:2926/1‑149 (MQ=255)
                             ccTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                     <  7:122645/149‑1 (MQ=255)
                              cTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                     <  7:23897/148‑1 (MQ=255)
                              cTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                     >  8:23897/1‑148 (MQ=255)
                                    gATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAggg                                                                                                                         >  3:255558/1‑138 (MQ=255)
                                    gATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAggg                                                                                                                         <  4:255558/138‑1 (MQ=255)
                                     aTGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTc                                                                                                             <  4:126087/149‑1 (MQ=255)
                                      tGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTc                                                                                                             <  7:215810/148‑1 (MQ=255)
                                            cTTCATCATTAGTGTCGGTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGGCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGGCTTCGTAAAtt                                                                                                      <  7:271773/149‑1 (MQ=255)
                                                atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGaa                                                                                                  >  1:136388/1‑149 (MQ=255)
                                                   atGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCt                                                                                               <  2:136388/149‑1 (MQ=255)
                                                   atGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCt                                                                                               >  2:142911/1‑149 (MQ=255)
                                                   atGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATCTTCCAGATCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCt                                                                                               >  7:173981/1‑149 (MQ=255)
                                                     gATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             >  5:51759/1‑149 (MQ=255)
                                                      aTTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             <  4:16964/148‑1 (MQ=255)
                                                                atgaGAAACTCCAAAATTTTACATTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGATTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATACGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGAt                                                                                  >  7:162336/1‑149 (MQ=255)
                                                                     aaaCTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACt                                                                             >  6:308285/1‑149 (MQ=255)
                                                                      aaCTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTACATTCGAACCTGGGa                                                                                           <  7:268346/134‑1 (MQ=255)
                                                                      aaCTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                           >  8:268346/1‑134 (MQ=255)
                                                                        cTCCAATATTTTACCTTCCCTGCGCAGGTCAGAGGACCGTGCACCTTGGGTTTCATGTTCCAGGTCATTGTCAATATGAGGTAGACGGTCCTTCAAATAGGGTAGTGAGTCTTCGTAATTTCGAACCTGGGAGCAGCCGATGGACTTGt                                                                          <  8:173981/149‑1 (MQ=255)
                                                                        cTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGt                                                                          <  1:142911/149‑1 (MQ=255)
                                                                        cTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGt                                                                          <  6:51759/149‑1 (MQ=255)
                                                                         tCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             <  1:95082/129‑1 (MQ=255)
                                                                         tCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             >  2:95082/1‑129 (MQ=255)
                                                                           cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                     <  8:70158/103‑1 (MQ=255)
                                                                           cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                     >  7:70158/1‑103 (MQ=255)
                                                                           cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTa                                                                       >  6:311416/1‑149 (MQ=255)
                                                                             aaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATg                                                                     <  7:52144/149‑1 (MQ=255)
                                                                                      cTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAt                                                            >  2:161825/1‑149 (MQ=255)
                                                                                      cTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAt                                                            >  6:70153/1‑149 (MQ=255)
                                                                                       ttCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACtt                                                                            >  3:187169/1‑132 (MQ=255)
                                                                                       ttCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACtt                                                                            <  4:187169/132‑1 (MQ=255)
                                                                                          ccAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCt                                                                                                               <  5:266255/94‑1 (MQ=255)
                                                                                          ccAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCt                                                                                                               >  6:266255/1‑94 (MQ=255)
                                                                                          ccAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAtttct                                                        <  1:2926/149‑1 (MQ=255)
                                                                                                   gTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAg                                               <  5:70153/149‑1 (MQ=255)
                                                                                                          gACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGc                                                                                                                                       >  1:169715/1‑54 (MQ=255)
                                                                                                          gACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGc                                                                                                                                       <  2:169715/54‑1 (MQ=255)
                                                                                                          gACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGa                                        >  2:258426/1‑149 (MQ=255)
                                                                                                                 cACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTc                                  <  1:161825/148‑1 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             <  5:157118/88‑1 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                             >  6:157118/1‑88 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTa                                                                <  2:268992/117‑1 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTa                                                                >  1:268992/1‑117 (MQ=255)
                                                                                                                          tttCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTc                        >  5:8032/1‑149 (MQ=255)
                                                                                                                          tttCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTc                        >  1:121821/1‑149 (MQ=255)
                                                                                                                                  tctaGCCCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACtt                                                                            <  3:50454/86‑1 (MQ=255)
                                                                                                                                  tctaGCCCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACtt                                                                            >  4:50454/4‑89 (MQ=255)
                                                                                                                                     aGCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGtt              >  1:57389/1‑148 (MQ=255)
                                                                                                                                      gCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGAAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGa            <  1:258426/149‑1 (MQ=255)
                                                                                                                                           tACTCAATATGATCTAGACGCTACATGAAATTGGTGATTGAGTCTTCTTAAATTCGAACATGGGAGCAGCCGATGCACTTGTCTATGCTGTATCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACt        <  5:101372/148‑1 (MQ=255)
                                                                                                                                                aaTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAtttt  >  4:302352/1‑149 (MQ=255)
                                                                                                                                                aaTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAtttt  >  3:298996/1‑149 (MQ=255)
                                                                                                                                                aaTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAtttt  >  5:326195/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTTT  >  NC_002947/2224689‑2224981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑