Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,230,996 A→T 8.0% E71D (GAA→GAT)  PP_1969 → molybdenum cofactor biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,230,9960AT 8.0% 145.5 / 3.5 50E71D (GAA→GAT) PP_1969molybdenum cofactor biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base T (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGGCGCGCAAGTTGCCG  >  NC_002947/2230848‑2231143
                                                                                                                                                     |                                                                                                                                                   
gcaggcGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGaa                                                                                                                                                     <  5:212637/149‑1 (MQ=255)
  aggcGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGaa                                                                                                                                                     <  1:91696/147‑1 (MQ=255)
  aggcGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGaa                                                                                                                                                     >  2:91696/1‑147 (MQ=255)
                ccTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGATGCCGGCGGCTTCGatc                                                                                                                                     <  4:212571/149‑3 (MQ=255)
                ccTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGATGCCGGCGGCTTCGat                                                                                                                                      >  3:212571/1‑147 (MQ=255)
                ccTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAgc                                                                                                                                     <  8:52432/149‑1 (MQ=255)
                            ccTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTa                                                                                                                         <  8:219696/149‑1 (MQ=255)
                               gACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTg                                                                                                                      >  3:7085/1‑149 (MQ=255)
                                     tgcctgcAACTACGCCTGTACCTACTGCGGTACTTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGt                                                                                                                 >  1:23126/1‑149 (MQ=255)
                                               tACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGt                                                                                                      >  4:179399/1‑149 (MQ=255)
                                                   ccTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGATGCCGGCGGCTTCGat                                                                                                                                      <  6:203043/113‑2 (MQ=255)
                                                   ccTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGATGCCGGCGGCTTCGat                                                                                                                                      >  5:203043/1‑112 (MQ=255)
                                                    cTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGcc                                                                                                 <  2:261773/149‑1 (MQ=255)
                                                    cTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGcc                                                                                                 >  3:22185/1‑149 (MQ=255)
                                                      gTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCg                                                                                                                   <  2:273573/129‑1 (MQ=255)
                                                      gTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCg                                                                                                                   >  1:273573/1‑129 (MQ=255)
                                                       tACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCcgc                                                                                              >  5:83351/1‑149 (MQ=255)
                                                            aCTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCt                                                                                          <  1:119172/148‑1 (MQ=255)
                                                            aCTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCt                                                                                          >  2:119172/1‑148 (MQ=255)
                                                                      cTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGc                                                                                                                    <  4:181311/113‑1 (MQ=255)
                                                                         aCGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTa                                                                               <  4:135652/147‑1 (MQ=255)
                                                                          cGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTAt                                                                              >  8:2994/1‑147 (MQ=255)
                                                                          cGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTAt                                                                              <  7:2994/147‑1 (MQ=255)
                                                                           ggAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGc                                                                           >  7:22283/1‑149 (MQ=255)
                                                                             aaaGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTg                                                                         >  3:69483/1‑149 (MQ=255)
                                                                                  ggCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCAt                                                                                                                                          <  4:19474/79‑1 (MQ=255)
                                                                                  ggCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCAt                                                                                                                                          >  3:19474/1‑79 (MQ=255)
                                                                                   gCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGc                                                                                                                    >  2:293849/1‑100 (MQ=255)
                                                                                   gCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGc                                                                                                                    <  1:293849/100‑1 (MQ=255)
                                                                                         ggcggcGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAAc                                                             >  3:34312/1‑149 (MQ=255)
                                                                                             gcgcAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGa                                                         <  3:179399/149‑1 (MQ=255)
                                                                                               gcAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGAcc                                                       >  8:285545/1‑149 (MQ=255)
                                                                                                 aGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCg                                                          >  6:262779/1‑144 (MQ=255)
                                                                                                 aGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCg                                                          <  5:262779/144‑1 (MQ=255)
                                                                                                  ggACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACAtt                                                     >  8:285242/1‑148 (MQ=255)
                                                                                                         cTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGt                                             <  8:22283/149‑1 (MQ=255)
                                                                                                            tCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGtctc                                          <  6:83351/149‑1 (MQ=255)
                                                                                                              ggcggATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCg                                         <  4:34312/148‑1 (MQ=255)
                                                                                                               gcggATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCt                                       <  4:22185/149‑1 (MQ=255)
                                                                                                                 ggATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGa                                     <  5:104243/149‑1 (MQ=255)
                                                                                                                        ttGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCaa                              >  6:83665/1‑149 (MQ=255)
                                                                                                                           gCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACg                            <  4:7085/148‑1 (MQ=255)
                                                                                                                            cccGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGc                          <  3:78650/149‑1 (MQ=255)
                                                                                                                             ccGTGGCGTAGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGc                          >  1:310929/1‑148 (MQ=255)
                                                                                                                                       gcCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTTGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGgc               <  4:69483/149‑1 (MQ=255)
                                                                                                                                       gcCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGgc               >  5:35847/1‑149 (MQ=255)
                                                                                                                                         cTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGtt                     >  7:67143/1‑141 (MQ=255)
                                                                                                                                         cTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGtt                     <  8:67143/141‑1 (MQ=255)
                                                                                                                                             cTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGgcgcgc           >  3:303347/1‑147 (MQ=255)
                                                                                                                                                  cGAAGCCGCCGGCATCGAGCGCCTGCGGTTTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATAACGTCTCGCTGACCTCCAACGGCCAGTTGCTGGCGCGCAAGTTGc    >  2:27457/1‑149 (MQ=255)
                                                                                                                                                   gAAGCCGCCGGCATCGAGCGCCTGCGGTTTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATAACGTCTCGCTGACCTCCAACGGCCAGTTGCTGGCGCGCAAGTTGc    <  1:27457/148‑1 (MQ=255)
                                                                                                                                                   gAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGGCGCGCAAGTTGcc   <  7:285545/149‑1 (MQ=255)
                                                                                                                                                   gAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGGCGCGCAAGTTGc    <  2:23126/148‑1 (MQ=255)
                                                                                                                                                     aGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGGCGCGCAAGTTGCCg  >  7:233707/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCAGGCGCTTTCGCAACCTGCGCGTCAGCCTGACGGCTGCCTGCAACTACGCCTGTACCTACTGC‑GTACCTGACGGAAAGCGGCTGGTGGCGGCGCAGGACGAGCTGTCGGCGGATGCCTTGGCCCGTGGCGTGGCCTACCTGATCGAAGCCGCCGGCATCGAGCGCCTGCGGATTACTGGCGGTGAGCCACTGGTCAGCCCGCGCCTGGATGCCTTTATGGCTGCCGTGGCCAAACTCGACCTTGATGACGTCTCGCTGACCACCAACGGCCAGTTGCTGGCGCGCAAGTTGCCG  >  NC_002947/2230848‑2231143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑