| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,235,038 | A→G | 6.6% | N50S (AAC→AGC) | uvrB → | uvrABC excinuclease protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,235,038 | 0 | A | G | 6.6% | 175.6 / 2.1 | 60 | N50S (AAC→AGC) | uvrB | uvrABC excinuclease protein B |
| Reads supporting (aligned to +/- strand): ref base A (30/26); new base G (2/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTCCGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGACTACTACCAGCCGGAAG > NC_002947/2234892‑2235184 | gTCCGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACg > 6:189530/1‑149 (MQ=255) tCCGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGt > 8:50327/1‑149 (MQ=255) tCCGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGt > 7:211011/1‑149 (MQ=255) cGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAGCGTc > 7:7635/1‑148 (MQ=255) cGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAGCGTCa > 7:3166/1‑149 (MQ=255) cGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCa < 3:272187/149‑1 (MQ=255) aGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATc < 2:7302/149‑1 (MQ=255) aGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCAGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCGCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATc > 6:284397/1‑149 (MQ=255) acGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGcc > 3:253577/1‑149 (MQ=255) cGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGccc < 5:284397/149‑1 (MQ=255) ttCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGcc < 6:99741/145‑1 (MQ=255) ttCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGcc > 5:99741/1‑145 (MQ=255) cAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCt > 1:187167/1‑149 (MQ=255) cGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTg > 7:263699/1‑149 (MQ=255) gCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGACGGCTTCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCAATGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCt < 7:171697/149‑1 (MQ=255) cTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTg < 3:102519/149‑1 (MQ=255) gCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGgcg > 1:291323/1‑149 (MQ=255) cAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGcc > 5:257375/1‑127 (MQ=255) cAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGcc < 6:257375/127‑1 (MQ=255) cAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCcaa < 4:67399/149‑1 (MQ=255) aGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCcaa > 4:89583/1‑148 (MQ=255) cGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGa < 2:187167/149‑1 (MQ=255) gCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGgccgcc > 7:252357/1‑149 (MQ=255) tCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTAc > 3:58593/1‑149 (MQ=255) gAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACg < 5:189530/148‑1 (MQ=255) gCATCGAGGCTGGTCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTGCGGGCAAGACCTTCATCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGTGAg < 4:88285/149‑1 (MQ=255) cATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGt < 3:89583/149‑1 (MQ=255) aTCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGt > 1:126565/1‑136 (MQ=255) gAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCaa < 4:58593/149‑1 (MQ=255) cTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCAttctt < 8:252357/149‑1 (MQ=255) gCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTcccc < 4:253577/148‑1 (MQ=255) gCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGAACTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCTGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTcccc < 8:263699/148‑1 (MQ=255) aGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTcc > 5:257030/1‑141 (MQ=255) aGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTcc < 6:257030/141‑1 (MQ=255) gCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAGCGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTg < 8:7635/148‑1 (MQ=255) gCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAGCGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTg < 8:3166/148‑1 (MQ=255) cTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTg > 7:34130/1‑147 (MQ=255) ggggTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTa < 2:291323/149‑1 (MQ=255) aCCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCAGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGt > 5:216829/1‑149 (MQ=255) gTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTcc > 2:186459/1‑149 (MQ=255) gTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTcc > 1:168556/1‑149 (MQ=255) ttCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCt < 8:211011/149‑1 (MQ=255) cGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTa < 7:50327/148‑1 (MQ=255) ggCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTa < 5:12655/149‑1 (MQ=255) gCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCCACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTAc > 6:178291/1‑149 (MQ=255) aCCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGa > 7:186816/1‑125 (MQ=255) aCCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGa < 8:186816/125‑1 (MQ=255) aCCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTAc < 7:208471/144‑1 (MQ=255) aCCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTAc > 8:208471/1‑144 (MQ=255) cTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGactac > 1:200257/1‑148 (MQ=255) ttCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTa < 6:128173/140‑1 (MQ=255) ttCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTa > 5:128173/1‑140 (MQ=255) ttCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTAc > 3:158483/1‑141 (MQ=255) ttCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTAc < 4:158483/141‑1 (MQ=255) ttCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGActacta > 3:39748/1‑149 (MQ=255) cATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCAttcttc > 1:186356/1‑100 (MQ=255) cATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCAttcttc < 2:186356/100‑1 (MQ=255) ttGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGACTACTACCAGCCg > 6:69330/1‑149 (MQ=255) ccAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGACTACTACCAGCCGGa > 1:113798/1‑148 (MQ=255) cAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGACTACTACCAGCCGGAAg > 6:112145/1‑149 (MQ=255) | GTCCGAGTTCCAGCTCGTCACACGTTTCCAGCCGGCTGGCGACCAGCCCGAGGCTATCCGCCAGTTGGTCGAAGGCATCGAGGCGGGGCTGTCGCACCAGACGTTGCTCGGGGTGACCGGTTCGGGCAAGACCTTCAGCATTGCCAACGTCATCCAGCAGGTGCAGCGCCCGACGCTGGTGCTGGCGCCCAACAAGACGCTGGCCGCCCAGTTGTACGGGGAGTTCAAGGCATTCTTCCCCAACAACGCGGTGGAGTACTTCGTTTCCTATTACGACTACTACCAGCCGGAAG > NC_002947/2234892‑2235184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |