Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,252,813 T→A 8.3% intergenic (+69/‑50) leuD → / → PP_1987 3‑isopropylmalate dehydratase small subunit/UbiE/COQ5 family methlytransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,252,8130TA 8.3% 137.5 / 2.5 48intergenic (+69/‑50)leuD/PP_19873‑isopropylmalate dehydratase small subunit/UbiE/COQ5 family methlytransferase
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base A (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

AGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGT  >  NC_002947/2252671‑2252955
                                                                                                                                              |                                                                                                                                              
aGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTAcc                                                                                                                                            >  4:15047/1‑147 (MQ=255)
aGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTAcc                                                                                                                                            <  3:15047/147‑1 (MQ=255)
aGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCcg                                                                                                                                          <  1:32006/149‑1 (MQ=255)
    cAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                      <  7:287218/149‑1 (MQ=255)
    cAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                      <  6:136390/149‑1 (MQ=255)
         aCGCGATCAAGGCCTTCGAAGCCAAACACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGc                                                                                                                                 >  8:158938/1‑149 (MQ=255)
             gATCATGGCCTTCTAAGCCAAGAACAGCGCCTGACAGCCTTGGCTGTTCCTTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCgg                                                                                                                              <  8:174190/148‑1 (MQ=255)
                 aaGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAg                                                                                                                         <  6:6933/149‑1 (MQ=255)
                 aaGGCCTTCGAAGCCAAACACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGcaca                                                                                                                          <  7:158938/148‑1 (MQ=255)
                  aGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAgg                                                                                                                        >  8:155918/1‑149 (MQ=255)
                   ggCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAgg                                                                                                                        >  8:133126/1‑148 (MQ=255)
                          gAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACaaaa                                                                                                                <  7:133126/149‑1 (MQ=255)
                          gAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACaaaa                                                                                                                <  7:155918/149‑1 (MQ=255)
                                 aGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGTGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTaaa                                                                                                         <  3:293048/149‑1 (MQ=255)
                                 aGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAAt                                                                                                              >  3:187794/1‑144 (MQ=255)
                                 aGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAAt                                                                                                              <  4:187794/144‑1 (MQ=255)
                                   cACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAgg                                                                                                                        <  4:49961/132‑1 (MQ=255)
                                   cACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAgg                                                                                                                        >  3:49961/1‑132 (MQ=255)
                                     ccGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGCTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTCTAGGCGCGGGTTTTCCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGaa                                                                                                     >  4:268404/1‑149 (MQ=255)
                                           cagccagcCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTaa                                                                                                          <  1:146741/138‑1 (MQ=255)
                                           cagccagcCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTaa                                                                                                          >  2:146741/1‑138 (MQ=255)
                                              ccagccTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACAt                                                                                             >  4:259304/1‑148 (MQ=255)
                                               cagccTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGa                                                                                           >  6:178144/1‑149 (MQ=255)
                                                    ttGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGATAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAccagc                                                                                      >  7:114364/1‑149 (MQ=255)
                                                         tGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGAc                                                                                               >  5:60283/1‑135 (MQ=255)
                                                         tGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGAc                                                                                               <  6:60283/135‑1 (MQ=255)
                                                               gTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACAt                                                                           >  6:227551/1‑149 (MQ=255)
                                                                      cTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtgtg                                                                    >  5:11364/1‑149 (MQ=255)
                                                                      cTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtgtg                                                                    >  5:80733/1‑149 (MQ=255)
                                                                             tCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCaa                                                              <  3:259304/148‑1 (MQ=255)
                                                                               gTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGGAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACg                                                            <  5:178144/148‑1 (MQ=255)
                                                                                              ggCCTCCTCTCTCCTACAGAAATTTACGCGGGCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTACAAAACTATAAAGGAACGAGTCATGACCATCACCCAGCACATCGATTTGGTCCAACGCCAGTTCGGCGAGCAg                                            <  8:239533/149‑1 (MQ=38)
                                                                                                    ctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAgc                                      >  6:13483/1‑149 (MQ=255)
                                                                                                              cAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTca                            <  6:80733/149‑1 (MQ=255)
                                                                                                                 aaaGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGCGGCCGGCACAGGCAACCAAAATCTAAAGGACCGAGACATGACCAGCACCCAGCCCATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTCCCTcag                           >  1:217313/1‑147 (MQ=39)
                                                                                                                       aCGCGGTCGCTGTAGGCGACGGTTTACCCGAGAAGAGGCCGCAACAGGCAACAAAAATCTAAAGCAACGAGAAATCAACAGCACACAGCACATAGATGTGGTCAAACGACAGATCCGCGATCAGCCCAGCGCCTACCTAAGCAGCGCCg                   >  2:11527/1‑149 (MQ=255)
                                                                                                                         gcgGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGt                  <  6:181667/148‑1 (MQ=255)
                                                                                                                         gcgGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGt                  >  5:181667/1‑148 (MQ=255)
                                                                                                                              cGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGcc                                                          <  1:174051/103‑1 (MQ=255)
                                                                                                                              cGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGcc                                                          >  2:174051/1‑103 (MQ=255)
                                                                                                                                   tcttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGcc                    >  7:67072/5‑136 (MQ=255)
                                                                                                                                   tcttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGcc                    >  7:71372/5‑136 (MQ=255)
                                                                                                                                   tcttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGcc                    <  8:67072/132‑1 (MQ=255)
                                                                                                                                   tcttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGcc                    <  8:71372/132‑1 (MQ=255)
                                                                                                                                   tAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAg       <  6:11364/149‑1 (MQ=255)
                                                                                                                                        gcgGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAACTCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGGCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCATCGCCGTGCACGCCCAAGGCAGt  <  2:217313/149‑1 (MQ=255)
                                                                                                                                            gTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGc                                                  <  4:133787/97‑1 (MQ=255)
                                                                                                                                            gTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGc                                                  >  3:133787/1‑97 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGT  >  NC_002947/2252671‑2252955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑