Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,280,705 C→T 5.9% V615V (GTC→GTT)  fadH → 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,280,7050CT 5.9% 204.5 / 2.0 70V615V (GTC→GTT) fadH2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base T (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GGATCAAGGCCGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCG  >  NC_002947/2280557‑2280852
                                                                                                                                                    |                                                                                                                                                   
ggATCAAGGCCGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTc                                                                                                                                                     <  7:108009/149‑1 (MQ=255)
   tCAAGGCCGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAg                                                                                                                                                  >  8:33929/1‑149 (MQ=255)
          cGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTgg                                                                                                                                           <  8:264399/149‑1 (MQ=255)
            aGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGt                                                                                                                                         <  5:9324/149‑1 (MQ=255)
             gCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTa                                                                                                                                        >  6:126686/1‑149 (MQ=255)
                 cATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATcga                                                                                                                                    <  2:232999/149‑1 (MQ=255)
                     cTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGAt                                                                                                                                >  1:166322/1‑149 (MQ=255)
                      tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCt                                                                                                                                             >  1:219703/1‑135 (MQ=255)
                      tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCt                                                                                                                                             <  2:219703/135‑1 (MQ=255)
                      tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATg                                                                                                                               >  1:143961/1‑149 (MQ=255)
                               gCAGGTGTACCTGCTGCAGCGCAAGAAAGCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGCGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGc                                                                                                                      <  8:113369/149‑1 (MQ=255)
                                   gtgtACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACAt                                                                                                                  <  2:166322/149‑1 (MQ=255)
                                     gtACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTc                                                                                                                <  2:32666/149‑1 (MQ=255)
                                      tACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTc                                                                                                                <  6:135744/148‑1 (MQ=255)
                                        ccTGTTGCAGCGCAAGAAATCCTAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCTCCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              <  1:311680/148‑1 (MQ=255)
                                        ccTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtg                                                                                                             >  8:283128/1‑149 (MQ=255)
                                        ccTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCCGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtg                                                                                                             >  8:62518/1‑149 (MQ=255)
                                         cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtgt                                                                                                            >  1:30320/1‑149 (MQ=255)
                                         cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              >  6:108346/1‑147 (MQ=255)
                                         cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              <  5:108346/147‑1 (MQ=255)
                                          tGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGACCATACGtgtg                                                                                                           >  8:276243/1‑149 (MQ=255)
                                           gTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGtgtg                                                                                                           >  6:301474/1‑148 (MQ=255)
                                            ttGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGa                                                                                                         <  7:283128/149‑1 (MQ=255)
                                            ttGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGa                                                                                                         <  7:62518/149‑1 (MQ=255)
                                                      aGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAg                                                                                               >  8:4030/1‑149 (MQ=255)
                                                          aTCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              <  1:235203/130‑1 (MQ=255)
                                                          aTCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              >  2:235203/1‑130 (MQ=255)
                                                            ccAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCTGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAg                                                                                         >  7:73424/1‑149 (MQ=255)
                                                              aaGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGt                                                                                       <  7:276243/149‑1 (MQ=255)
                                                                   gggCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTgg                                                                                  >  8:89702/1‑149 (MQ=255)
                                                                       gACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCTGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGt                                                                              <  8:73424/149‑1 (MQ=255)
                                                                        aCGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTg                                                                             <  2:30320/149‑1 (MQ=255)
                                                                         cGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTgg                                                                            <  5:301474/149‑1 (MQ=255)
                                                                           ggCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGa                                                                           >  6:93830/1‑148 (MQ=255)
                                                                                 gCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACgtg                                                                    <  8:162066/149‑1 (MQ=255)
                                                                                  cAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACgtgg                                                                   >  6:86435/1‑149 (MQ=255)
                                                                                   aaGANGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGcc                                                                                             >  5:13817/1‑122 (MQ=255)
                                                                                   aaGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGcc                                                                                             <  6:13817/122‑1 (MQ=255)
                                                                                   aaGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCATGTGCTGGCGGTGGATAACgtggt                                                                  <  7:89702/149‑1 (MQ=255)
                                                                                      acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                               >  8:179445/1‑149 (MQ=255)
                                                                                      acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                               <  2:143961/149‑1 (MQ=255)
                                                                                      acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                               <  1:303642/149‑1 (MQ=255)
                                                                                        gacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCt                                                             >  4:179543/1‑149 (MQ=255)
                                                                                           cGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCtgtg                                                          <  5:86435/149‑1 (MQ=255)
                                                                                             gCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGcc                                                        <  7:179445/149‑1 (MQ=255)
                                                                                                 gATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCggg                                                     >  7:246285/1‑148 (MQ=255)
                                                                                                 gATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCgg                                                      <  5:126686/147‑1 (MQ=255)
                                                                                                  aTTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCa                                                   <  7:241152/149‑1 (MQ=255)
                                                                                                      accgcaccgGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGc                                                    >  6:53049/1‑144 (MQ=255)
                                                                                                      accgcaccgGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGc                                                    >  1:109979/1‑144 (MQ=255)
                                                                                                      accgcaccgGGTTGAAGAACAACGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGTATCACGTGGTGATCTGTGCCGGGc                                                    >  4:25141/1‑144 (MQ=255)
                                                                                                                    aagaaCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              <  6:209381/72‑1 (MQ=255)
                                                                                                                    aagaaCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                              >  5:209381/1‑72 (MQ=255)
                                                                                                                     agaaCAAGCGGGTGCAGCTGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTTTGGACGTCGGCGAGCCACAGGTGCTGGAGGTGGATAACGAGGTGATCTGTGCCGTGCAAGATCCGCTGCGCGAGATg                                >  2:99147/1‑149 (MQ=255)
                                                                                                                      gaaCAAGGGGGTGCAGATGCTCAACAGTGTGGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTg                                >  4:264135/1‑148 (MQ=255)
                                                                                                                        aCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGGATCGACGATGCCGGCCTGCACATACGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAg                             >  7:122530/1‑149 (MQ=255)
                                                                                                                          aaGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAgg                            >  6:228493/1‑148 (MQ=255)
                                                                                                                            gggggTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAg                         >  5:63020/1‑149 (MQ=255)
                                                                                                                               tacccAGATACTCGACACTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAATGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGc                      >  6:107472/5‑149 (MQ=255)
                                                                                                                               ggTTCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGc                      <  3:25141/149‑1 (MQ=255)
                                                                                                                                   cAGATGCGCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCCGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCtggt                  <  6:87647/149‑1 (MQ=255)
                                                                                                                                    aGATGCTCAACAGTGTGGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCtggtg                 <  3:264135/149‑1 (MQ=255)
                                                                                                                                        acTCGACACTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTggcgg             >  3:78283/2‑149 (MQ=255)
                                                                                                                                            gacacTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAATGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTggcggtg           <  5:107472/146‑3 (MQ=255)
                                                                                                                                            gacacTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGcggcgg          <  4:78283/147‑1 (MQ=255)
                                                                                                                                            atcAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGgcggcg           <  7:139453/145‑1 (MQ=255)
                                                                                                                                            aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCggg         >  8:18259/1‑149 (MQ=255)
                                                                                                                                            aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCggg         >  5:95372/1‑149 (MQ=255)
                                                                                                                                            aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGAGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGgcggcg           >  8:139453/1‑147 (MQ=255)
                                                                                                                                                   tCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCg  <  6:63020/149‑1 (MQ=255)
                                                                                                                                                   tCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCg  >  4:309988/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGATCAAGGCCGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCG  >  NC_002947/2280557‑2280852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑