Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,280,709 T→C 5.7% Y617H (TAT→CAT)  fadH → 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,280,7090TC 5.7% 209.3 / 2.0 70Y617H (TAT→CAT) fadH2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

CGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCGGTG  >  NC_002947/2280567‑2280855
                                                                                                                                              |                                                                                                                                                  
cGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTgg                                                                                                                                              <  8:264399/149‑1 (MQ=255)
  aGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGt                                                                                                                                            <  5:9324/149‑1 (MQ=255)
   gCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTa                                                                                                                                           >  6:126686/1‑149 (MQ=255)
       cATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATcga                                                                                                                                       <  2:232999/149‑1 (MQ=255)
           cTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGAt                                                                                                                                   >  1:166322/1‑149 (MQ=255)
            tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCt                                                                                                                                                >  1:219703/1‑135 (MQ=255)
            tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCt                                                                                                                                                <  2:219703/135‑1 (MQ=255)
            tCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATg                                                                                                                                  >  1:143961/1‑149 (MQ=255)
                     gCAGGTGTACCTGCTGCAGCGCAAGAAAGCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGCGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGc                                                                                                                         <  8:113369/149‑1 (MQ=255)
                         gtgtACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACAt                                                                                                                     <  2:166322/149‑1 (MQ=255)
                           gtACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTc                                                                                                                   <  2:32666/149‑1 (MQ=255)
                            tACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTc                                                                                                                   <  6:135744/148‑1 (MQ=255)
                              ccTGTTGCAGCGCAAGAAATCCTAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCTCCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 <  1:311680/148‑1 (MQ=255)
                              ccTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtg                                                                                                                >  8:283128/1‑149 (MQ=255)
                              ccTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCCGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtg                                                                                                                >  8:62518/1‑149 (MQ=255)
                               cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgtgt                                                                                                               >  1:30320/1‑149 (MQ=255)
                               cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 <  5:108346/147‑1 (MQ=255)
                               cTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 >  6:108346/1‑147 (MQ=255)
                                tGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGACCATACGtgtg                                                                                                              >  8:276243/1‑149 (MQ=255)
                                 gTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGtgtg                                                                                                              >  6:301474/1‑148 (MQ=255)
                                  ttGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGa                                                                                                            <  7:62518/149‑1 (MQ=255)
                                  ttGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGa                                                                                                            <  7:283128/149‑1 (MQ=255)
                                            aGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAg                                                                                                  >  8:4030/1‑149 (MQ=255)
                                                aTCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 >  2:235203/1‑130 (MQ=255)
                                                aTCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 <  1:235203/130‑1 (MQ=255)
                                                  ccAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCTGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAg                                                                                            >  7:73424/1‑149 (MQ=255)
                                                    aaGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGt                                                                                          <  7:276243/149‑1 (MQ=255)
                                                         gggCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTgg                                                                                     >  8:89702/1‑149 (MQ=255)
                                                             gACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCTGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGt                                                                                 <  8:73424/149‑1 (MQ=255)
                                                              aCGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTg                                                                                <  2:30320/149‑1 (MQ=255)
                                                               cGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTgg                                                                               <  5:301474/149‑1 (MQ=255)
                                                                 ggCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGa                                                                              >  6:93830/1‑148 (MQ=255)
                                                                       gCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACgtg                                                                       <  8:162066/149‑1 (MQ=255)
                                                                        cAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACgtgg                                                                      >  6:86435/1‑149 (MQ=255)
                                                                         aaGANGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGcc                                                                                                >  5:13817/1‑122 (MQ=255)
                                                                         aaGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGcc                                                                                                <  6:13817/122‑1 (MQ=255)
                                                                         aaGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCATGTGCTGGCGGTGGATAACgtggt                                                                     <  7:89702/149‑1 (MQ=255)
                                                                            acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                                  <  2:143961/149‑1 (MQ=255)
                                                                            acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                                  <  1:303642/149‑1 (MQ=255)
                                                                            acgacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGAt                                                                  >  8:179445/1‑149 (MQ=255)
                                                                              gacCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCt                                                                >  4:179543/1‑149 (MQ=255)
                                                                                 cGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCtgtg                                                             <  5:86435/149‑1 (MQ=255)
                                                                                   gCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGcc                                                           <  7:179445/149‑1 (MQ=255)
                                                                                       gATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCggg                                                        >  7:246285/1‑148 (MQ=255)
                                                                                       gATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCgg                                                         <  5:126686/147‑1 (MQ=255)
                                                                                        aTTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCa                                                      <  7:241152/149‑1 (MQ=255)
                                                                                            accgcaccgGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGc                                                       >  6:53049/1‑144 (MQ=255)
                                                                                            accgcaccgGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGc                                                       >  1:109979/1‑144 (MQ=255)
                                                                                            accgcaccgGGTTGAAGAACAACGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGTATCACGTGGTGATCTGTGCCGGGc                                                       >  4:25141/1‑144 (MQ=255)
                                                                                                          aagaaCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 >  5:209381/1‑72 (MQ=255)
                                                                                                          aagaaCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCgt                                                                                                                 <  6:209381/72‑1 (MQ=255)
                                                                                                           agaaCAAGCGGGTGCAGCTGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTTTGGACGTCGGCGAGCCACAGGTGCTGGAGGTGGATAACGAGGTGATCTGTGCCGTGCAAGATCCGCTGCGCGAGATg                                   >  2:99147/1‑149 (MQ=255)
                                                                                                            gaaCAAGGGGGTGCAGATGCTCAACAGTGTGGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTg                                   >  4:264135/1‑148 (MQ=255)
                                                                                                              aCAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGGATCGACGATGCCGGCCTGCACATACGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAg                                >  7:122530/1‑149 (MQ=255)
                                                                                                                aaGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAgg                               >  6:228493/1‑148 (MQ=255)
                                                                                                                  gggggTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAg                            >  5:63020/1‑149 (MQ=255)
                                                                                                                     tacccAGATACTCGACACTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAATGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGc                         >  6:107472/5‑149 (MQ=255)
                                                                                                                     ggTTCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGc                         <  3:25141/149‑1 (MQ=255)
                                                                                                                         cAGATGCGCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCCGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCtggt                     <  6:87647/149‑1 (MQ=255)
                                                                                                                          aGATGCTCAACAGTGTGGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCtggtg                    <  3:264135/149‑1 (MQ=255)
                                                                                                                              acTCGACACTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTggcgg                >  3:78283/2‑149 (MQ=255)
                                                                                                                                  gacacTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAATGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTggcggtg              <  5:107472/146‑3 (MQ=255)
                                                                                                                                  gacacTGTTGAGCATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGcggcgg             <  4:78283/147‑1 (MQ=255)
                                                                                                                                  atcAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGgcggcg              <  7:139453/145‑1 (MQ=255)
                                                                                                                                  aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCggg            >  8:18259/1‑149 (MQ=255)
                                                                                                                                  aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCggg            >  5:95372/1‑149 (MQ=255)
                                                                                                                                  aaCAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGAGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGgcggcg              >  8:139453/1‑147 (MQ=255)
                                                                                                                                         tCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCg     <  6:63020/149‑1 (MQ=255)
                                                                                                                                         tCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCg     >  4:309988/1‑149 (MQ=255)
                                                                                                                                            aGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCGGTg  >  4:203219/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CGAGCCGCATGCTCCGGCGCGGCAGGTGTACCTGTTGCAGCGCAAGAAATCCAAGGTGGGCGACGGGCTCGGCAAGACGACCGGCTGGATTCACCGCACCGGGTTGAAGAACAAGGGGGTGCAGATGCTCAACAGTGTCGAGTATCTGGGTATCGACGATGCCGGCCTGCACATTCGTGTGGACGGCGGCGAGCCCCAGGTGCTGGCGGTGGATAACGTGGTGATCTGTGCCGGGCAAGATCCGCTGCGCGAGCTGCAGGAAGGGCTGGTGGCGGCGGGGCAGTCGGTG  >  NC_002947/2280567‑2280855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑