Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 255,258 A→G 8.3% intergenic (‑142/+21) PP_0203 ← / ← PP_t74 dipeptidase/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947255,2580AG 8.3% 134.1 / 2.3 48intergenic (‑142/+21)PP_0203/PP_t74dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCT  >  NC_002947/255119‑255401
                                                                                                                                           |                                                                                                                                               
ttGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAggg                                                                                                                                        <  4:102668/149‑1 (MQ=255)
     cgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttt                                                                                                                                   <  5:303759/149‑2 (MQ=255)
             tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttcgttt                                                                                                                             <  8:79783/147‑8 (MQ=38)
             tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttcgttt                                                                                                                             >  7:79783/1‑140 (MQ=38)
               tGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATgg                                                                                                                          <  4:128208/148‑1 (MQ=255)
                 ccAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGgcg                                                                                                                        >  2:174703/1‑148 (MQ=255)
                  cAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCa                                                                                                                      >  2:287296/1‑149 (MQ=255)
                   aaTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCttt                                                                                                                                    <  1:60488/134‑1 (MQ=255)
                   aaTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCttt                                                                                                                                    >  2:60488/1‑134 (MQ=255)
                    aTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACt                                                                                                                    >  3:29984/1‑149 (MQ=255)
                        ttGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggc                                                                                                                >  1:10736/1‑149 (MQ=255)
                           ttCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcgg                                                                                                              >  7:2257/1‑148 (MQ=255)
                                     cccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGatat                                                                                                                            >  7:215710/1‑124 (MQ=255)
                                     cccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGatat                                                                                                                            <  8:215710/124‑1 (MQ=255)
                                      ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                  >  3:148321/1‑149 (MQ=255)
                                      ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                  >  2:119223/1‑149 (MQ=255)
                                             ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcgg                                                                                                              <  4:294395/130‑1 (MQ=255)
                                             ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcgg                                                                                                              >  3:294395/1‑130 (MQ=255)
                                             ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcg                                                                                                               >  3:49145/1‑129 (MQ=255)
                                             ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcg                                                                                                               <  4:49145/129‑1 (MQ=255)
                                                 aCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGcc                                                                                       >  7:1761/1‑149 (MQ=255)
                                                       aGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGa                                                                                 <  8:1761/149‑1 (MQ=255)
                                                          cGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                              <  2:10736/149‑1 (MQ=255)
                                                           gCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGc                                                                             <  1:287296/149‑1 (MQ=255)
                                                           gCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGAACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                              >  4:160125/1‑148 (MQ=255)
                                                                cGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                        <  4:29984/149‑1 (MQ=255)
                                                                       acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATc                                                                  >  4:253664/1‑148 (MQ=255)
                                                                         cAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGTACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAg                                                               <  3:160125/149‑1 (MQ=255)
                                                                                ccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGc                                                         >  3:244911/1‑148 (MQ=255)
                                                                                  gTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTAc                                                      <  1:119223/149‑1 (MQ=255)
                                                                                  gTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTAc                                                      <  1:174703/149‑1 (MQ=255)
                                                                                       aCGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                              <  3:162151/120‑1 (MQ=255)
                                                                                         gggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCg                                                                                   <  7:217292/113‑1 (MQ=255)
                                                                                         gggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCg                                                                                   >  8:217292/1‑113 (MQ=255)
                                                                                           gAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                             >  8:171500/1‑149 (MQ=255)
                                                                                              tACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAcgcg                                          >  3:266844/1‑149 (MQ=255)
                                                                                                 cAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                       >  4:109968/1‑149 (MQ=255)
                                                                                                   gAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacg                                      >  4:131662/1‑148 (MQ=255)
                                                                                                        gTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGAt                                <  3:109968/149‑1 (MQ=255)
                                                                                                               gTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGGTTTATTGATATGGCGCACTCGGCGGGATTCGATCCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATCCCCATc                         >  4:55890/1‑149 (MQ=255)
                                                                                                                   cacaAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtgtg                     <  3:253664/149‑1 (MQ=255)
                                                                                                                       aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                 >  5:32452/1‑149 (MQ=255)
                                                                                                                          gAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGa              >  1:189283/1‑149 (MQ=255)
                                                                                                                           ataaaGCCCTGGGCCTGAGCCCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc             >  2:54659/3‑149 (MQ=39)
                                                                                                                             aaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt           <  3:131662/149‑1 (MQ=255)
                                                                                                                                gCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCa        <  4:148321/149‑1 (MQ=255)
                                                                                                                                 ccccGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCAt       <  6:32452/149‑1 (MQ=255)
                                                                                                                                      ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCt  <  7:171500/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
TTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCT  >  NC_002947/255119‑255401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑