Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,304,497 C→G 8.9% L115L (CTG→CTC)  PP_2027 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,304,4970CG 8.9% 136.8 / 4.9 45L115L (CTG→CTC) PP_2027hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/20);  new base G (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TGCTGTCGTCGTACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGGTGTGCAGGCTGTTCAGCTGTCGCGTG  >  NC_002947/2304352‑2304644
                                                                                                                                                 |                                                                                                                                                    
tgctgTCGTCGTACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGAGTTGTACTGGAGGTTGGAATCGGACTGAAGAACTTGCatt                                                                                                                                                   >  4:115054/1‑147 (MQ=255)
   tgTCGTCGTACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTc                                                                                                                                                 >  8:282429/1‑148 (MQ=255)
    gtcgtcGTACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGa                                                                                                                                               <  3:255839/149‑1 (MQ=255)
        tcgtACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑Gc                                                                                                                                          <  5:129504/149‑1 (MQ=255)
        gagtAAAGCTCTACCGAGTGGTAGATGCGCAGGGTCTGGACTGCTTCTGCCTGGCCATAGACGTCGTGTGTGTCTATGGTGTCTTCGAGGAATACCTGAGCGTTGTCCTGGCTGTTGGCATCGTACTGCAGAACTTGCAGTTCGCTG‑Gc                                                                                                                                          <  6:250466/147‑1 (MQ=255)
           tACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTg                                                                                                                                       >  7:26904/1‑149 (MQ=255)
           tACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTg                                                                                                                                       <  8:26904/149‑1 (MQ=255)
                  cTACCGAGTGGTAGATGCGCAGGGTCAGCCCGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGc                                                                                                                                <  5:283445/149‑1 (MQ=255)
                     ccGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGAc                                                                                                                             >  4:233719/1‑149 (MQ=255)
                       gAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTg                                                                                                                           <  2:27505/149‑1 (MQ=255)
                                        gggcAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCgg                                                                                                          <  6:157316/146‑1 (MQ=255)
                                        ggTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCgg                                                                                                          <  3:233719/149‑1 (MQ=255)
                                            aGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGGAGCTCGGGGAt                                                                                                      >  8:199244/1‑149 (MQ=255)
                                             gCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTgg                                                                                                                                      >  6:184257/1‑116 (MQ=255)
                                             gCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTgg                                                                                                                                      <  5:184257/116‑1 (MQ=255)
                                             gCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATg                                                                                                     >  2:65085/1‑149 (MQ=255)
                                                      gggCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATGCGCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAgg                                                                                               <  1:65085/147‑1 (MQ=255)
                                                      gggCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTgg                                                                                                                          >  3:134529/1‑119 (MQ=255)
                                                      gggCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTgg                                                                                                                          >  3:129756/1‑119 (MQ=255)
                                                      gggCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTgg                                                                                                                          <  4:129756/119‑1 (MQ=255)
                                                      gggCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTgg                                                                                                                          <  4:134529/119‑1 (MQ=255)
                                                        gCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCgg                                                                                                          >  6:225835/1‑133 (MQ=255)
                                                        gCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCgg                                                                                                          <  5:225835/133‑1 (MQ=255)
                                                                   gACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTg                                                                               >  5:260421/1‑149 (MQ=255)
                                                                    aCGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTgg                                                                              <  7:282429/149‑1 (MQ=255)
                                                                      gtcgtGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGgca                                                                            >  7:147622/1‑149 (MQ=255)
                                                                           ggCTGTCGAGGGTGGCTTCAATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAgg                                                                       >  3:235581/1‑149 (MQ=255)
                                                                              tGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGGAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGt                                                                     >  3:57286/1‑148 (MQ=255)
                                                                              tGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGt                                                                     >  1:262796/1‑148 (MQ=255)
                                                                                     ggTGGCTTCAATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATcc                                                             <  4:235581/149‑1 (MQ=255)
                                                                                          cTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCgg                                                        <  8:147622/149‑1 (MQ=255)
                                                                                              gATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGAACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGc                                                    >  5:97492/1‑149 (MQ=255)
                                                                                               aTGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCt                                                   >  7:156049/1‑149 (MQ=255)
                                                                                                    tACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCAc                                              <  5:43181/149‑1 (MQ=255)
                                                                                                         gAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGtt                                         >  7:21101/1‑149 (MQ=255)
                                                                                                           gCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTc                                       <  6:260421/149‑1 (MQ=255)
                                                                                                                       cGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGgt                           <  3:115054/149‑1 (MQ=255)
                                                                                                                        ggTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGgtg                          >  8:276756/1‑149 (MQ=255)
                                                                                                                               aTCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTAc                                                                                           <  7:82095/77‑1 (MQ=255)
                                                                                                                               aTCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTAc                                                                                           >  8:82095/1‑77 (MQ=255)
                                                                                                                                   gCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGGTGTGCAGGCTGtt               >  6:289468/1‑149 (MQ=255)
                                                                                                                                        cagcaCTTGGAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGGTGTGCAGGCTGTTCAGc           <  4:57286/148‑1 (MQ=255)
                                                                                                                                              ttGGAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAgg                             >  3:91717/1‑124 (MQ=255)
                                                                                                                                              ttGGAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAgg                             <  4:91717/124‑1 (MQ=255)
                                                                                                                                                gCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGGTGTGCAGGCTGTTCAGCTGTCGCGTg  >  7:300260/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGCTGTCGTCGTACAGCGCTACCGAGTGGTAGATGCGCAGGGTCAGCACGGCTTGGGCCTGGACATAGACGTCGTGGCTGTCGAGGGTGGCTTCGATGAATACCTGAGCGTTGTCCTGGCGGTTGGCATCGGCCTGCAGCACTTGCAGTTCGATG‑GCCTGGCTGTGCGACTGGCCCAGCCGCAGCTCGGGGATGCGCAGGGTACCGCTGCGCCGTGGCAGCAGGGTAATGATCCAGCGGGTGCTGGCACGGGTTTCGCCGTCCAGGGTGTGCAGGCTGTTCAGCTGTCGCGTG  >  NC_002947/2304352‑2304644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑